Japanese Macaques as Laboratory Animals

Isa, Tadashi; Yamane, Itaru; Hamai, Miya; Inagaki, Haruhisa

doi:10.1538/expanim.58.451

Cited by 27 publications

(22 citation statements)

References 11 publications

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“…14080 México, D.F., México;ibanez.alejandra@hotmail.com, jany2104@hotmail.com Los primates no humanos (PNH) son un excelente modelo para el estudio de los mecanismos neurales fundamentales en la función auditiva (Torre y col 2000, Populin 2006, Fowler y col 2010, esto es, por la posición que ocupan en el árbol evolutivo, siendo la especie animal más similar filogenéticamente al humano (Wilson y Sarich 1969, Groves 2001, Fowler y col 2010. Dentro del orden de los primates la especie más utilizada en la investigación biomédica son los monos rhesus (Macaca mulatta) (Segal 1989, Schapiro 1996, Rowe 1996, Jones 1999, Kemnitz y col 2000, Groves 2001, Isa 2009). Se estima que el tiempo de vida de estos organismos es de aproximadamente 30 años en condiciones de cautiverio (Rowe 1996, AnkelSimons 2000, lo que facilita y complementa los estudios de tipo longitudinal, para el conocimiento de procesos tales como desarrollo, maduración y envejecimiento de la vía auditiva.…”

Section: Introductionunclassified

Potenciales evocados auditivos del tallo cerebral en monos rhesus (Macaca mulatta) en diferentes etapas fisiológicas en condiciones de cautiverio

et al. 2011

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ARTÍCULO ORIGINAL Potenciales evocados auditivos del tallo cerebral en monos rhesus (Macaca mulatta) en diferentes etapas fisiológicas en condiciones de cautiverio SUMMARYIn the phylogenetic scale, audition plays a very important role in the development of elaborated behaviors. The aim of this study was to evaluate the auditive response in the Macaca mulatta species at different physiologic stages, through brainstem's auditory evoked potentials (BAEP). 30 non-human primates Macaca mulatta were allotted into two groups of 15 males and 15 females distributed in five age-dependant groups of 2 males and 2 females as follows: Group 1 (0,1-3,1 year old); Group 2 (3,2-6,1 year old); Group 3 (6,2-9,1 year old); Group 4 (9,2-12,1 year old) and Group 5 (12,2-27,1 years and older). The BAEP were obtained by the stimulation of the ear with rarefaction "clicks" of 50 dB of intensity. The cerebral electric activity was picked up by gold disc electrodes, placed in the Cz (+), A1, A2 (-) and Fz derivations as land, according to the 10/20 international system. Four waves were detected in the five evaluated groups. Since no significant differences were found in the ANOVAs by separated afferences, an ANOVA of united afferences was done. It was observed that groups 1 and 5 provided significant differences in all evaluated waves, showing the extended latencies in relation to other groups. It was concluded that BAEP made it possible to identify changes that are generated from the development, maturation and aging in rhesus monkeys.Palabras clave: generadores neurales auditivos, latencias, potenciales evocados auditivos de tallo cerebral.

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Section: Introductionunclassified

Potenciales evocados auditivos del tallo cerebral en monos rhesus (Macaca mulatta) en diferentes etapas fisiológicas en condiciones de cautiverio

et al. 2011

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“…In Japan, a project is in progress to establish Japanese macaques as laboratory animals. 17 Therefore, Japanese macaques may serve as an animal model of T-cell lymphomas in humans.…”

Section: Discussionmentioning

confidence: 99%

Characterization of Spontaneous Malignant Lymphomas in Japanese Macaques (Macaca fuscata)

et al. 2014

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Lymphomas are common spontaneous tumors in nonhuman primates but remain poorly characterized in Japanese macaques (Macaca fuscata). This study examined 5 cases of spontaneous malignant lymphoma in Japanese macaques, focusing on the immunophenotypes and presence of simian lymphocryptoviruses, which are Epstein-Barr virus-related herpesviruses in nonhuman primates. The macaques with lymphoma were 5 to 28 years old, indicating that lymphomas develop over a wide age range. The common macroscopic findings were splenomegaly and enlargement of lymph nodes. Histologic and immunohistochemical analyses revealed that all cases were non-Hodgkin type and exhibited a T-cell phenotype, positive for CD3 but negative for CD20 and CD79a. The lymphomas exhibited diverse cellular morphologies and were subdivided into 3 types according to the World Health Organization classification. These included 3 cases of peripheral T-cell lymphoma, not otherwise specified; 1 case of T-cell prolymphocytic leukemia; and 1 case of an unclassifiable T-cell lymphoma. Positive signals were detected by in situ hybridization in 2 of the 4 examined cases using probes for the Epstein-Barr virus-encoded small RNA (EBER). Furthermore, the presence of M. fuscata lymphocryptovirus 2, a macaque homolog of Epstein-Barr virus, was demonstrated in EBER-positive cases by polymerase chain reaction amplification followed by direct sequencing. Immunohistochemistry using antibody to the Epstein-Barr virus-encoded nuclear antigen 2 was negative, even in the EBER-positive cases. The present study suggests that T-cell lymphoma is more common than B-cell lymphoma in Japanese macaques and that M. fuscata lymphocryptovirus 2 is present in some cases.

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“…200,000 clones was constructed. Although a complete genome sequence of Japanese macaques is not yet available [11], terminal sequences of the inserted genes have already been analyzed and chromosome mapping of BAC clones are accessible in a database held at the DNA Data Bank of Japan (DDBJ) of the National Institute of Genetics. The MSM/Ms mouse BAC clones have contributed to SNP analysis of Asian mouse subspecies Mus musculus molossinus and the C57Bl/6J mouse strain by BAC-end sequence analysis [1].…”

Section: Bac Clonesmentioning

confidence: 99%

Genetic Materials at the Gene Engineering Division, RIKEN BioResource Center

et al. 2010

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Genetic materials are one of the most important and fundamental research resources for studying biological phenomena. Scientific need for genetic materials has been increasing and will never cease. Ever since it was established as RIKEN DNA Bank in 1987, the Gene Engineering Division of RIKEN BioResource Center (BRC) has been engaged in the collection, maintenance, storage, propagation, quality control, and distribution of genetic resources developed mainly by the Japanese research community. When RIKEN BRC was inaugurated in 2001, RIKEN DNA Bank was incorporated as one of its six Divisions, the Gene Engineering Division. The Gene Engineering Division was selected as a core facility for the genetic resources of mammalian and microbe origin by the National BioResource Project (NBRP) of the Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan in 2002. With support from the scientific community, the Division now holds over 3 million clones of genetic materials for distribution. The genetic resources include cloned DNAs, gene libraries (e.g., cDNA and genomic DNA cloned into phage, cosmid, BAC, phosmid, and YAC), vectors, hosts, recombinant viruses, and ordered library sets derived from animal cells, including human and mouse cells, microorganisms, and viruses. Recently genetic materials produced by a few MEXT national research projects were transferred to the Gene Engineering Division for further dissemination. The Gene Engineering Division performs rigorous quality control of reproducibility, restriction enzyme mapping and nucleotide sequences of clones to ensure the reproducibility of in vivo and in vitro experiments. Users can easily access our genetic materials through the internet and obtain the DNA resources for a minimal fee. Not only the materials, but also information of features and technology related to the materials are provided via the web site of RIKEN BRC. Training courses are also given to transfer the technology for handling viral vectors. RIKEN BRC supports scientists around the world in the use of valuable genetic materials.

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Japanese Macaques as Laboratory Animals

Cited by 27 publications

References 11 publications

Potenciales evocados auditivos del tallo cerebral en monos rhesus (Macaca mulatta) en diferentes etapas fisiológicas en condiciones de cautiverio

Potenciales evocados auditivos del tallo cerebral en monos rhesus (Macaca mulatta) en diferentes etapas fisiológicas en condiciones de cautiverio

Characterization of Spontaneous Malignant Lymphomas in Japanese Macaques (Macaca fuscata)

Genetic Materials at the Gene Engineering Division, RIKEN BioResource Center

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