Japanese encephalitis protein vaccine candidates expressing neutralizing epitope and M.T hsp70 induce virus-specific memory B cells and long-lasting antibodies in swine

Ge, Fei-Fei; Wang, Jian; Feng, Xin; Sheng, Li‐Ping; Sun, Qiangming; Zhou, Jinlin; Pu-yan, Chen; Liu, Peihong

doi:10.1016/j.vaccine.2008.07.104

Cited by 7 publications

(6 citation statements)

References 24 publications

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“…However, we found that mice immunized with 5 ϫ 10 6 PFU of rVSV-HSP70-VP1 generated significantly higher T cell immunity than those immunized with rVSV-VP1 (P Ͻ 0.05). Previously, it has been shown that HSP70 can enhance the serum IgG antibody response to a number of viruses, including RSV (41), Hantaan virus (40), and JE virus (38,39). Unexpectedly, we did not observe a significant increase in NoV-specific serum IgG antibody or HBGA blocking antibody levels in any of the experiments, suggesting that HSP70 plays a minor role in the NoVspecific IgG response.…”

Section: Hsp70 Enhances the Safety Of Vsv-based Human Nov Vaccinecontrasting

confidence: 52%

“…HSP70 enhances the immunogenicity of the viral vaccine. HSP70 has been shown to be a strong adjuvant for a number of viral vaccines, including porcine reproductive and respiratory syndrome virus (PRRSV) (37), Japanese encephalitis (JE) virus (38,39), Hantaan virus (40), respiratory syncytial virus (RSV) (41), and measles virus (24). PRRSV causes immunosuppression in pigs.…”

Section: Hsp70 Enhances the Safety Of Vsv-based Human Nov Vaccinementioning

confidence: 99%

“…Fusion of HSP70 to the nucleocapsid protein (NP) of Hantaan virus (HSP70-NP) or codelivery of HSP70 and NP proteins (HSP70ϩNP) elicited significantly higher NP-specific antibody titers and higher frequencies of IFN-␥-producing cells and CTL activities in vivo than conventional NP vaccination alone (40). HSP70 also enhanced T lymphocyte proliferation and IFN-␥ and IL-4 secretion and produced virus-specific memory B cells and long-lasting antibodies when fused with the envelope (E) protein of JE virus (38).…”

Section: Hsp70 Enhances the Safety Of Vsv-based Human Nov Vaccinementioning

confidence: 99%

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Heat Shock Protein 70 Enhances Mucosal Immunity against Human Norovirus When Coexpressed from a Vesicular Stomatitis Virus Vector

Duan

Wei

et al. 2014

J Virol

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IMPORTANCEHuman norovirus (NoV) is responsible for more than 95% of acute nonbacterial gastroenteritis worldwide. Currently, there is no vaccine for this virus. Development of a live attenuated vaccine for human NoV has not been possible because it is uncultivable. Thus, a live vector-based vaccine may provide an alternative vaccine strategy. In this study, we developed a vesicular stomatitis virus (VSV)-based human NoV vaccine candidate. We constructed rVSV-HSP70-VP1, coexpressing heat shock protein (HSP70) and capsid (VP1) genes of human NoV, and rVSV-Luc-VP1, coexpressing firefly luciferase (Luc) and VP1 genes. We found that VSVs with a double gene insertion were significantly more attenuated than VSV with a single VP1 insertion (rVSV-VP1). Furthermore, we found that coexpression or coadministration of HSP70 from VSV vector significantly enhanced human NoV-specific mucosal immunity. Collectively, we developed an improved live vectored vaccine candidate for human NoV which will be useful for future clinical studies.

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Section: Hsp70 Enhances the Safety Of Vsv-based Human Nov Vaccinecontrasting

confidence: 52%

Section: Hsp70 Enhances the Safety Of Vsv-based Human Nov Vaccinementioning

confidence: 99%

Section: Hsp70 Enhances the Safety Of Vsv-based Human Nov Vaccinementioning

confidence: 99%

See 1 more Smart Citation

Heat Shock Protein 70 Enhances Mucosal Immunity against Human Norovirus When Coexpressed from a Vesicular Stomatitis Virus Vector

Duan

Wei

et al. 2014

J Virol

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“…For example, the anti-Japanese encephalitis virus vaccine consists of fusion protein between viral envelope protein E (neutralizing epitope) and heat shock protein Hsp70. This protein induces virus-specific memory B cells and longlasting antibodies in swine more efficiently than the vaccine with neutralizing epitope alone [101]. Hsp90 is as well a candidate for an antiviral vaccine component.…”

Section: Insight Into Viral Pathogenesismentioning

confidence: 99%

Proteomics for development of vaccine

Adamczyk-Popławska

Markowicz

Jagusztyn-Krynicka

2011

Journal of Proteomics

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“…Both inactivated and live attenuated vaccines have been employed, the latter appearing to be most effective [ 70 ]. There is a large body of research on newer vaccine candidates, including recombinant protein [ 71 ], plasmid DNA [ 72 ], lentiviral vector [ 73 ], virus-like particle [ 74 ], mRNA lipid nanoparticle vaccine [ 75 ], and recombinant live vaccines based on the Genotype I backbone [ 67 , 76 ]. It is unlikely that any of the veterinary vaccines currently used in the endemic region would be acceptable for importation and use in the US in an emergency without considerable additional study, and the newer candidates are early in development.…”

Section: Vaccines As a Countermeasurementioning

confidence: 99%

Japanese Encephalitis: Risk of Emergence in the United States and the Resulting Impact

Monath

2023

Viruses

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Japanese encephalitis virus is a mosquito-borne member of the Flaviviridae family. JEV is the leading cause of viral encephalitis in Asia and is characterized by encephalitis, high lethality, and neurological sequelae in survivors. The virus also causes severe disease in swine, which are an amplifying host in the transmission cycle, and in horses. US agricultural authorities have recently recognized the threat to the swine industry and initiated preparedness activities. Other mosquito-borne viruses exotic to the Western Hemisphere have been introduced and established in recent years, including West Nile, Zika, and chikungunya viruses, and JEV has recently invaded continental Australia for the first time. These events amply illustrate the potential threat of JEV to US health security. Susceptible indigenous mosquito vectors, birds, feral and domestic pigs, and possibly bats, constitute the receptive ecological ingredients for the spread of JEV in the US. Fortunately, unlike the other virus invaders mentioned above, an inactivated whole virus JE vaccine (IXIARO®) has been approved by the US Food and Drug Administration for human use in advance of a public health emergency, but there is no veterinary vaccine. This paper describes the risks and potential consequences of the introduction of JEV into the US, the need to integrate planning for such an event in public health policy, and the requirement for additional countermeasures, including antiviral drugs and an improved single dose vaccine that elicits durable immunity in both humans and livestock.

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Japanese encephalitis protein vaccine candidates expressing neutralizing epitope and M.T hsp70 induce virus-specific memory B cells and long-lasting antibodies in swine

Cited by 7 publications

References 24 publications

Heat Shock Protein 70 Enhances Mucosal Immunity against Human Norovirus When Coexpressed from a Vesicular Stomatitis Virus Vector

Heat Shock Protein 70 Enhances Mucosal Immunity against Human Norovirus When Coexpressed from a Vesicular Stomatitis Virus Vector

Proteomics for development of vaccine

Japanese Encephalitis: Risk of Emergence in the United States and the Resulting Impact

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