Jaagsiekte sheep retrovirus is present at high concentration in lung fluid produced by ovine pulmonary adenocarcinoma-affected sheep and can survive for several weeks at ambient temperatures

Cousens, Chris; Thonur, Leenadevi; Imlach, Stuart; Crawford, Joanne; Sales, Jill; Griffiths, David J.

doi:10.1016/j.rvsc.2008.11.007

Cited by 45 publications

(37 citation statements)

References 14 publications

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“…The virus can survive in the environment for many days (Cousens and others 2009) and, therefore, any contaminated areas such as holding pens should be disinfected and, if possible, grazing areas should not be re-used for sheep for at least several weeks.…”

Section: Flock Management and Control Measuresmentioning

confidence: 99%

Diagnosis and control of ovine pulmonary adenocarcinoma (Jaagsiekte)

Scott¹,

Griffiths²,

Cousens³

2013

In Practice

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Ovine pulmonary adenocarcinoma (OPA) is a contagious tumour of the lungs of sheep. It is also commonly known as Jaagsiekte, ovine pulmonary carcinoma or sheep pulmonary adenomatosis. OPA is generally considered a chronic wasting disease with progressive respiratory distress leading to emaciation, but it may be that early lung lesions predispose to secondary bacterial pneumonia causing sudden death despite antibiotic treatment. OPA is common in the UK and most other countries where sheep are farmed, but the disease may be grossly under-reported because few deaths are investigated on sheep farms. Disease transmission is facilitated by close confinement such as indoor housing. Effective control, and possible disease eradication, has been hampered by the lack of a suitable diagnostic test or vaccine. This article describes the clinical features of OPA and outlines current methods of diagnosis. Issues surrounding the development of improved diagnostic tests are discussed.

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Section: Flock Management and Control Measuresmentioning

confidence: 99%

Diagnosis and control of ovine pulmonary adenocarcinoma (Jaagsiekte)

Scott¹,

Griffiths²,

Cousens³

2013

In Practice

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“…All samples were run in duplicate with 100 ng of RNA in a 20-l final reaction volume. Primers and amplification conditions used were as described previously (13).…”

Section: Preparation Of Jsrvmentioning

confidence: 99%

“…Harvested supernatant was then filtered through a 0.45-m cellulose acetate filter (Sartorius) and concentrated by ultracentrifugation through a glycerol cushion at 100,000 ϫ g for 3 h before resuspending the viral pellet in phosphate-buffered saline (PBS) at a 200ϫ concentration. The virus was quantified by qRT-PCR as described previously (13). Each lamb received a dose equivalent to approximately 10 10 RNA copies of JSRV 21 in a total volume of 4 ml.…”

mentioning

confidence: 99%

Jaagsiekte Sheep Retrovirus Infects Multiple Cell Types in the Ovine Lung

Martineau

Cousens

Imlach

et al. 2011

J Virol

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“…The disease is characterized by fluid accumulation in the lungs, which results from the production of excess secretions by tumor cells. Filtered lung fluid from infected sheep contains viral particles, and this fluid is able to manifest the disease when inoculated intratracheally into healthy sheep [6]. OPA is the most common pulmonary tumor that affects sheep and occurs in many countries around the world, with the exception of Australia, New Zealand, and Iceland.…”

Section: Introductionmentioning

confidence: 99%

Diagnosis and phylogenetic analysis of ovine pulmonary adenocarcinoma in China

et al. 2013

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Ovine pulmonary adenocarcinoma (OPA) is a lung tumor of sheep caused by jaagsiekte sheep retrovirus (JSRV). OPA is common in sheep, and it is most commonly observed in China. Without preventative vaccines and serological diagnostic tools for assay of OPA, identification of JSRV based on reverse transcription polymerase chain reaction (RT-PCR) is very important for prevention and control measures for OPA in practice management. In this study, the diagnosis of OPA was made from analysis of clinical signs, pathological observations, JSRV-like particle discovery, and RT-PCR of the target env gene. The phylogenetic analysis showed that the China Shandong (SD) strain studied in this article belonged to exogenous JSRV, and it was very similar to 92k3, which was isolated from sheep in the Kenya (Y18305). The current study reported a severe outbreak of OPA in Shandong Province, China. The observations could offer a comparative view of the env gene of JSRV.

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Jaagsiekte sheep retrovirus is present at high concentration in lung fluid produced by ovine pulmonary adenocarcinoma-affected sheep and can survive for several weeks at ambient temperatures

Cited by 45 publications

References 14 publications

Diagnosis and control of ovine pulmonary adenocarcinoma (Jaagsiekte)

Diagnosis and control of ovine pulmonary adenocarcinoma (Jaagsiekte)

Jaagsiekte Sheep Retrovirus Infects Multiple Cell Types in the Ovine Lung

Diagnosis and phylogenetic analysis of ovine pulmonary adenocarcinoma in China

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