IVF versus ICSI for the fertilization of in-vitro matured human oocytes

Walls, Melanie; Junk, Stephen; Ryan, J. P.; Hart, Roger

doi:10.1016/j.rbmo.2012.08.001

Cited by 60 publications

(34 citation statements)

References 25 publications

(29 reference statements)

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“…Thus, the initial steps involved in oocyte activation may also be bypassed. In several species such as mice, humans, and rabbits, ICSI can activate oocytes for further embryonic development, comparable to IVF [10], [28], [29]. In bovine and porcine embryos, the developmental competencies of embryos followed by ICSI were low.…”

Section: Discussionmentioning

confidence: 99%

Birth of Healthy Offspring following ICSI in In Vitro-Matured Common Marmoset (Callithrix jacchus) Oocytes

et al. 2014

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Intracytoplasmic sperm injection (ICSI), an important method used to treat male subfertility, is applied in the transgenic technology of sperm-mediated gene transfer. However, no study has described successful generation of offspring using ICSI in the common marmoset, a small non-human primate used as a model for biomedical translational research. In this study, we investigated blastocyst development and the subsequent live offspring stages of marmoset oocytes matured in vitro and fertilized by ICSI. To investigate the optimal timing of performing ICSI, corrected immature oocytes were matured in vitro and ICSI was performed at various time points (1–2 h, 2–4 h, 4–6 h, 6–8 h, and 8–10 h after extrusion of the first polar body (PB)). Matured oocytes were then divided randomly into two groups: one was used for in vitro fertilization (IVF) and the other for ICSI. To investigate in vivo development of embryos followed by ICSI, 6-cell- to 8-cell-stage embryos and blastocysts were nonsurgically transferred into recipient marmosets. Although no significant differences were observed in the fertilization rate of blastocysts among ICSI timing after the first PB extrusion, the blastocyst rate at 1–2 h was lowest among groups at 2–4 h, 4–6 h, 6–8 h, and 8–10 h. Comparing ICSI to IVF, the fertilization rates obtained in ICSI were higher than in IVF (p>0.05). No significant difference was noted in the cleaved blastocyst rate between ICSI and IVF. Following the transfer of 37 ICSI blastocysts, 4 of 20 recipients became pregnant, while with the transfer of 21 6-cell- to 8-cell-stage ICSI embryos, 3 of 8 recipients became pregnant. Four healthy offspring were produced and grew normally. These are the first marmoset offspring produced by ICSI, making it an effective fertilization method for marmosets.

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Section: Discussionmentioning

confidence: 99%

Birth of Healthy Offspring following ICSI in In Vitro-Matured Common Marmoset (Callithrix jacchus) Oocytes

et al. 2014

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“…This would correspond with the time at which the E 2 levels in serum are lower (11), but, to our knowledge, no data are available in the literature indicating the E 2 and P 4 concentrations in OF at every specific time of the estrous cycle. From these observations, the first two hypotheses proposed in the present study are [1] that OF collected before and after ovulation would differentially affect the IVF results and [2] that steroid concentrations in OF collected at these specific time points vary in a different way from those concentrations already described in blood serum.…”

mentioning

confidence: 83%

“…Moreover, it has been suggested that the utilization of IVF in conjunction with in vitro maturation (IVM) treatment (avoiding the risks associated with ovarian stimulation with gonadotropins) is a suitable, more cost-effective and less invasive method for creating more viable embryos with improved implantation and pregnancy potential than ICSI (1). These reasons support the interest of studies addressed to increase the efficiency of IVF methods in humans.…”

mentioning

confidence: 99%

Timing of oviductal fluid collection, steroid concentrations, and sperm preservation method affect porcine in vitro fertilization efficiency

Ballester

Romero-Aguirregomezcorta

Soriano‐Úbeda

et al. 2014

Fertility and Sterility

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“…Oocyte IVM is a promising assisted reproductive technology for the treatment of human infertility and livestock embryo production (Gilchrist 2011;Walls et al 2012). However, the subsequent developmental competence of oocytes matured in vitro is compromised compared with in vivo-matured counterparts (Trounson et al 2001;Jurema and Nogueira 2006).…”

Section: Introductionmentioning

confidence: 99%

Effect of C-type natriuretic peptide on maturation and developmental competence of immature mouse oocytes in vitro

Wei

Zhou

Yuan

et al. 2017

Reprod. Fertil. Dev.

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In vitro maturation (IVM) of oocytes is an important assisted reproductive technology for infertility treatment and livestock breeding programs. Because of asynchronous nuclear and cytoplasmic maturation, the developmental competence of oocytes matured in vitro is compromised. C-Type natriuretic peptide (CNP), which has been proved to be an inhibitor of oocyte maturation, provides a new alternative to optimise synchronisation of nuclear and cytoplasmic maturation and improve developmental capacity of immature oocytes in vitro. To investigate the effect of temporary meiotic arrest mediated by CNP on maturation and subsequent development of immature oocytes, immature mouse oocytes from small antral follicles were temporarily arrested in meiosis by CNP (0, 5, 10 and 50nM) for 24h and then matured for 16h. CNP treatment significantly increased the oocyte maturation rate from less than half to above 80%. After IVF, temporary meiotic arrest mediated by 10 and 50nM CNP significantly improved fertilisation and blastocyst rate of oocytes matured in vitro up to approximately 55% and 30% respectively. Moreover, this positive effect of CNP was attributed, in part, to an increase in the number of mature oocytes with aligned chromosomes and a normal spindle. The present findings indicate the potential to use CNP to improve the efficiency of oocyte IVM.

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IVF versus ICSI for the fertilization of in-vitro matured human oocytes

Cited by 60 publications

References 25 publications

Birth of Healthy Offspring following ICSI in In Vitro-Matured Common Marmoset (Callithrix jacchus) Oocytes

Birth of Healthy Offspring following ICSI in In Vitro-Matured Common Marmoset (Callithrix jacchus) Oocytes

Timing of oviductal fluid collection, steroid concentrations, and sperm preservation method affect porcine in vitro fertilization efficiency

Effect of C-type natriuretic peptide on maturation and developmental competence of immature mouse oocytes in vitro

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