Ivermectin Increases Random-Pattern Skin Flap Survival in Rats: The Novel Role of GABAergic System

Tabary, Mohammadreza; Aryannejad, Armin; Noroozi, Nafise; Tavangar, Seyed Mohammad; Jafari, Razieh Mohammad; Araghi, Farnaz; Dadkhahfar, Sahar; Dehpour, Ahmad Reza

doi:10.1016/j.jss.2020.09.010

Cited by 21 publications

(30 citation statements)

References 58 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The survival of skin flaps is threatened by mal-perfusion, mal-oxygenation, low excretion and retention of tissue waste, necrosis and apoptosis, ischemia-induced injury, reperfusion injury, ROS injury, and inflammatory processes. It seems much more useful to avoid the creation of flap threatening processes and factors, rather than confronting them such as prevention of tissue necrosis via reduction of ROS production, elimination of created ROS, increasing of total antioxidant capacity (TAC) of flap tissue, and even use of exogenous antioxidants [ 10 , 15 , 36 , 39 , 40 ].…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, the activity of MPO enzyme is a manifestation of inflammation and chemotaxis of neutrophils. In addition to other effects of inflammation, these ROS also cause damage to the skin flap tissue [ 1 , 36 , 49 ]. In our study, skin flap surgery increased MPO activity, but in the groups that received Saffron, the enzyme activity level decreased significantly and resulted in an increase of skin flap survival.…”

Section: Discussionmentioning

confidence: 99%

“…Then, on day seven after surgery, the animals were sacrificed by injecting ketamine at a dose of 150 mg/kg. Tissue sampling was performed by excising a full-thickness skin from the intermediate margin between the necrotic area and the grossly healthy area [ 36 ]. The flaps were divided into two parts by a precise longitudinal incision, one component used to measure Mean MDA level, MPO and SOD activity, Bax, Bcl-2, mTOR, and p-mTOR expression (kept at − 80 ° C).…”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

The Novel Role of Crocus sativus L. in Enhancing Skin Flap Survival by Affecting Apoptosis Independent of mTOR: A Data-Virtualized Study

et al. 2022

View full text Add to dashboard Cite

Background Despite the improvements to enhance skin flap viability, the effects of ischemia-reperfusion (IR), oxidative stress, necrosis, and apoptosis are still challenging. Crocus sativus L. (Saffron) is highly noticeable due to its tissue-protective and antioxidant properties. So, we aimed to investigate its effects on skin flap viability, oxidative stress, apoptosis markers, histopathological changes, and mTOR/p-mTOR expression. Materials and Methods 40 Sprauge-Dawley rats, weighting 200–240 g, were divided into four groups including: (1) Sham (8 × 3 cm skin cut, without elevation); (2) Flap Surgery (8 × 3 cm skin flap with elevation from its bed); (3) Saffron 40 mg/kg + Flap Surgery; and (4) Saffron 80 mg/kg + Flap Surgery. Saffron was administrated orally for 7 days. At day 7, flap necrosis percentage, histopathological changes, malondialdehyde level, Myeloperoxidase and superoxide dismutase activity, Bax, Bcl-2, mTOR, and p-mTOR expression were measured. Protein expressions were controlled by β -Actin. Results Saffron administration decreased flap necrosis percentage ( p < 0.01), which was not dose-dependent. Treatment groups showed significant histological healing signs (Neovascularization, Fibroblast migration, Epithelialization, and Epithelialization thickness), decreased MDA content ( p < 0.01), increased SOD ( p < 0.01) and decreased MPO activity ( p < 0.01). Bax and Bcl-2 expression, decreased and increased respectively in treated groups ( p < 0.0001). mTOR and p-mTOR expression were not changed significantly in Saffron treated groups. Conclusion Saffron could increase skin flap viability, alleviate necrosis, decrease oxidative stress and decrease apoptotic cell death, after skin flap surgery, but it acts independent of the mTOR pathway. So, Saffron could potentially be used clinically to enhance skin flap viability. No Level Assigned This journal requires that authors assign a level of evidence to each submission to which Evidence-Based Medicine rankings are applicable. This excludes Review Articles, Book Reviews, and manuscripts that concern Basic Science, Animal Studies, Cadaver Studies, and Experimental Studies. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266. https://www.springer.com/00266 Supplementary Information The online version contains supplementary material available at 10.1007/s00266-022-03048-6.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

The Novel Role of Crocus sativus L. in Enhancing Skin Flap Survival by Affecting Apoptosis Independent of mTOR: A Data-Virtualized Study

et al. 2022

View full text Add to dashboard Cite

show abstract

“…Animals were housed in groups of 5 mice per cage. The behavioral tests were done between 9 a.m. and 3 p.m. in a group consisting of 10 mice, and each mouse was used only once [ 7 , 8 ]. The rodents were allowed 1-week habituation in the experimental environment.…”

Section: Methodsmentioning

confidence: 99%

Protective Effects of Dapsone on Scopolamine-Induced Memory Impairment in Mice: Involvement of Nitric Oxide Pathway

Noroozi

Shayan

Maleki

et al. 2022

Dement Geriatr Cogn Disord Extra

Self Cite

View full text Add to dashboard Cite

Introduction: The leading cause of memory impairment is dementia-related disorders. Since current treatments for memory impairment target the neuroinflammatory pathways, we selected dapsone, an anti-inflammatory agent, to evaluate its effects on scopolamine-induced memory impairment in mice and the underlying role of nitric oxide (NO). Methods: Scopolamine (1 mg/kg, intraperitoneal [i.p.]) was used for induction of memory impairment. The animals received various doses of dapsone (0.1, 0.3, 1, 5, and 10 mg/kg, i.p.). Duration and number of arms visits in the Y-maze and step-through latency in the passive-avoidance were documented. To evaluate the underlying signaling pathway, N(ω)-nitro-L-arginine methyl ester (a nonspecific NO synthase [NOS] inhibitor), aminoguanidine (a specific inducible NOS inhibitor), and 7-nitroindazole (a specific neuronal NOS inhibitor) were administered 30 min after dapsone administration. Results: Dapsone (5 mg/kg) substantially improved memory acquisition in scopolamine-induced memory impairment. Additionally, NOS inhibitors considerably reversed the observed neuroprotective effects of dapsone, accompanied by the elevation of NO levels. Conclusion: Dapsone revealed a neuroprotective effect against scopolamine-induced memory impairment in mice, possibly through the nitrergic pathway.

show abstract

“…22 We performed IHC staining for CD31 as described in detail previously. 22 Primary CD31 antibodies (1:100 dilution; Santa Cruz Biotechnology, Inc., TX) and secondary goat antirat antibodies (1:100 dilution; Santa Cruz Biotechnology, Inc., TX) were used for IHC staining of the specimens. After preparing and staining the samples, the slides were assessed by an expert pathologist blinded to treatment groups at ×200 and ×400 magnifications using an Olympus light microscope BH2 (Olympus Corporation, Tokyo, Japan), and capillary densities were compared among groups in regions far from the granulation tissue to avoid interruptions (at least five fields in each specimen were explored).…”

Section: Methodsmentioning

confidence: 99%

Cannabidiol Improves Random-Pattern Skin Flap Survival in Rats: Involvement of Cannabinoid Type-2 Receptors

Aryannejad

Eslami

Shayan

et al. 2022

J Reconstr Microsurg

Self Cite

View full text Add to dashboard Cite

Background One of the major complications associated with random-pattern skin flaps is distal necrosis. Cannabidiol (CBD) has recently gained much attention as a therapeutic anti-inflammatory agent. We aimed to evaluate the efficacy of CBD on the random-pattern skin flap survival (SFS) in rats and to explore the possible involvement of cannabinoid type-2 (CB2) receptors. Methods In this controlled experimental study, we randomly divided male Wistar rats into seven study groups (six rats each). We performed a random-pattern skin flap model in each rat following pretreatment with vehicle (control) or multiple doses of CBD (0.3, 1, 5, or 10 mg/kg). In a separate group, we injected SR144528 (2 mg/kg), a high affinity and selective CB2 inverse agonist, before the most effective dose of CBD (1 mg/kg). A sham nontreated and nonoperated group was also included. Seven days after surgeries, the percentage of necrotic area (PNA) was calculated. Histopathological microscopy, CB2 expression level, and interleukin (IL)-1β and tumor necrosis factor (TNF)-α concentrations were also investigated in the flap tissue samples. Results A PNA of 72.7 ± 7.5 (95% confidence interval [CI]: 64.8–80.6) was captured in the control group. Following treatment with CBD 0.3, 1, 5, and 10 mg/kg, a dose-dependent effect was observed with PNAs of 51.0 ± 10.0 (95% CI: 40.5–61.5; p <0.05), 15.4 ± 5.8 (95% CI: 9.3–21.5; p <0.001), 37.1 ± 10.2 (95% CI: 26.3–47.8; p <0.001), and 46.4 ± 14.0 (95% CI: 31.7–61.1; p <0.001), respectively. Histopathologically, tissues enhanced significantly. Besides, CB2 expression surged remarkably, IL-1β and TNF-α concentrations decreased considerably after treatment with CBD of 1 mg/kg compared with the control (p <0.05 and <0.001, respectively). Administering SR144528 reversed the favorable effects of CBD of 1 mg/kg, both macroscopically and microscopically. Conclusion Pretreatment with CBD of 1 mg/kg improved SFS considerably in rats and exerted desirable anti-inflammatory effects which were possibly mediated by CB2 receptors.

show abstract

Ivermectin Increases Random-Pattern Skin Flap Survival in Rats: The Novel Role of GABAergic System

Cited by 21 publications

References 58 publications

The Novel Role of Crocus sativus L. in Enhancing Skin Flap Survival by Affecting Apoptosis Independent of mTOR: A Data-Virtualized Study

The Novel Role of Crocus sativus L. in Enhancing Skin Flap Survival by Affecting Apoptosis Independent of mTOR: A Data-Virtualized Study

Protective Effects of Dapsone on Scopolamine-Induced Memory Impairment in Mice: Involvement of Nitric Oxide Pathway

Cannabidiol Improves Random-Pattern Skin Flap Survival in Rats: Involvement of Cannabinoid Type-2 Receptors

Contact Info

Product

Resources

About