ITS-polymorphism of salt-tolerant and salt-sensitive native isolates of Sinorhizoblum meliloti-symbionts of alfalfa, clover and fenugreek plants

Roumiantseva, M. L.; Muntyan, Victoria S.; Mengoni, Alessio; Симаров, Б. В.

doi:10.1134/s1022795414040103

Cited by 9 publications

(5 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…When examining the structure of AFLP patterns of isolates in relation to geographic variables, alongside the substantial variation ascribed to differences among isolates, as consistently found in S. meliloti populations [8,48,55,58], sharp clustering according to the sampling region was observed. This confirms that the genetic differentiation of rhizobial populations is mainly ascribed to geographic isolation, as previously found by other authors [29,33,35,55], thereby enabling application of the classic isolation-by-distance model [62] to nodulating rhizobial populations.…”

Section: Discussionmentioning

confidence: 89%

“…In Sinorhizobium natural population studies, it is usually recognized that isolates can significantly differ in their pH and salt tolerance, and indigenous strains can tolerate lower pH values and higher salt concentration [11,14,48,58]; on the other hand, in Croatia, there are many acidic soils and salinity can differ as well; therefore, it was important to check these phenotypes in the strains isolated in the present study. Table 1 summarizes results of phenotypic characterization of isolates (detailed in Table S4).…”

Section: Phenotypic Characterization Of Strainsmentioning

confidence: 97%

See 1 more Smart Citation

Biogeography of Sinorhizobium meliloti nodulating alfalfa in different Croatian regions

Donnarumma

Bazzicalupo

Blažinkov

et al. 2014

Research in Microbiology

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 89%

Section: Phenotypic Characterization Of Strainsmentioning

confidence: 97%

Biogeography of Sinorhizobium meliloti nodulating alfalfa in different Croatian regions

Donnarumma

Bazzicalupo

Blažinkov

et al. 2014

Research in Microbiology

View full text Add to dashboard Cite

“…The strains and plasmids used in this work are listed in Table 1 and Supplementary Table S1 . In particular a collection of S. meliloti strains from different geographical areas was used (Carelli et al, 2000 ; Roumiantseva et al, 2002 , 2014 ; Talebi Bedaf et al, 2008 ; Trabelsi et al, 2009 , 2010 ). S. meliloti strains were cultured on solid or liquid TY medium with 0.2 g/liter CaCO 3 , or Vincent Minimal Medium (VMM, or Rhizobium Defined Medium, RDM), while Escherichia coli strains were grown in Luria Bertani (LB) medium, supplemented with antibiotics when necessary.…”

Section: Methodsmentioning

confidence: 99%

Role and Regulation of ACC Deaminase Gene in Sinorhizobium meliloti: Is It a Symbiotic, Rhizospheric or Endophytic Gene?

et al. 2017

View full text Add to dashboard Cite

Plant-associated bacteria exhibit a number of different strategies and specific genes allow bacteria to communicate and metabolically interact with plant tissues. Among the genes found in the genomes of plant-associated bacteria, the gene encoding the enzyme 1-aminocyclopropane-1-carboxylate (ACC) deaminase (acdS) is one of the most diffused. This gene is supposed to be involved in the cleaving of plant-produced ACC, the precursor of the plant stress-hormone ethylene toning down the plant response to infection. However, few reports are present on the actual role in rhizobia, one of the most investigated groups of plant-associated bacteria. In particular, still unclear is the origin and the role of acdS in symbiotic competitiveness and on the selective benefit it may confer to plant symbiotic rhizobia. Here we present a phylogenetic and functional analysis of acdS orthologs in the rhizobium model-species Sinorhizobium meliloti. Results showed that acdS orthologs present in S. meliloti pangenome have polyphyletic origin and likely spread through horizontal gene transfer, mediated by mobile genetic elements. When acdS ortholog from AK83 strain was cloned and assayed in S. meliloti 1021 (lacking acdS), no modulation of plant ethylene levels was detected, as well as no increase in fitness for nodule occupancy was found in the acdS-derivative strain compared to the parental one. Surprisingly, AcdS was shown to confer the ability to utilize formamide and some dipeptides as sole nitrogen source. Finally, acdS was shown to be negatively regulated by a putative leucine-responsive regulator (LrpL) located upstream to acdS sequence (acdR). acdS expression was induced by root exudates of both legumes and non-leguminous plants. We conclude that acdS in S. meliloti is not directly related to symbiotic interaction, but it could likely be involved in the rhizospheric colonization or in the endophytic behavior.

show abstract

“…in the Mugodzhary region in 2002. This region is a part of a modern center of alfalfa intrоgressive hybridization in northwest Kazakhstan, which has been suffering from manmade salinization since the 1960s (1–3).…”

Section: Announcementmentioning

confidence: 99%

Draft Genome Sequence of Sinorhizobium meliloti Strain AK170

Батурина

Muntyan²,

Cherkasova³

et al. 2019

Microbiol Resour Announc

View full text Add to dashboard Cite

show abstract

ITS-polymorphism of salt-tolerant and salt-sensitive native isolates of Sinorhizoblum meliloti-symbionts of alfalfa, clover and fenugreek plants

Cited by 9 publications

References 44 publications

Biogeography of Sinorhizobium meliloti nodulating alfalfa in different Croatian regions

Biogeography of Sinorhizobium meliloti nodulating alfalfa in different Croatian regions

Role and Regulation of ACC Deaminase Gene in Sinorhizobium meliloti: Is It a Symbiotic, Rhizospheric or Endophytic Gene?

Draft Genome Sequence of Sinorhizobium meliloti Strain AK170

Contact Info

Product

Resources

About