iTRAQ protein profile analysis provides integrated insight into mechanisms of tolerance to TMV in tobacco (Nicotiana tabacum)

Wang, Jing; Wang, Xiaoran; Zhou, Qi; Yang, Jingquan; Guo, Haipeng; Yang, Lijun; Liu, Weiqun

doi:10.1016/j.jprot.2015.11.009

Cited by 37 publications

(32 citation statements)

References 38 publications

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“…Isobaric tags for relative and absolute quantification (iTRAQ) analysis, a gel-free protein quantitative approach containing isotope labeling, has developed into one of the primary proteomic tools. Owing to its technical advantages of high accuracy and sensitivity and more-accurate quantification, iTRAQ had been successfully used in the investigation of DAPS under various abiotic stress conditions including temperature stress (Liu et al, 2014, 2017; Zhang et al, 2017), aluminum stress (Wang et al, 2014) and salt stress (Fan et al, 2015; Li et al, 2015; Xu et al, 2015a; Chen et al, 2016; Jiang et al, 2017), as well as biotic stresses like tobacco mosaic virus (TMV) infection (Wang et al, 2016) and powdery mildew infection (Fu et al, 2016). …”

Section: Introductionmentioning

confidence: 99%

Unraveling the Root Proteome Changes and Its Relationship to Molecular Mechanism Underlying Salt Stress Response in Radish (Raphanus sativus L.)

Sun

Wang

et al. 2017

Front. Plant Sci.

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To understand the molecular mechanism underlying salt stress response in radish, iTRAQ-based proteomic analysis was conducted to investigate the differences in protein species abundance under different salt treatments. In total, 851, 706, and 685 differential abundance protein species (DAPS) were identified between CK vs. Na100, CK vs. Na200, and Na100 vs. Na200, respectively. Functional annotation analysis revealed that salt stress elicited complex proteomic alterations in radish roots involved in carbohydrate and energy metabolism, protein metabolism, signal transduction, transcription regulation, stress and defense and transport. Additionally, the expression levels of nine genes encoding DAPS were further verified using RT-qPCR. The integrative analysis of transcriptomic and proteomic data in conjunction with miRNAs was further performed to strengthen the understanding of radish response to salinity. The genes responsible for signal transduction, ROS scavenging and transport activities as well as several key miRNAs including miR171, miR395, and miR398 played crucial roles in salt stress response in radish. Based on these findings, a schematic genetic regulatory network of salt stress response was proposed. This study provided valuable insights into the molecular mechanism underlying salt stress response in radish roots and would facilitate developing effective strategies toward genetically engineered salt-tolerant radish and other root vegetable crops.

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Section: Introductionmentioning

confidence: 99%

Unraveling the Root Proteome Changes and Its Relationship to Molecular Mechanism Underlying Salt Stress Response in Radish (Raphanus sativus L.)

Sun

Wang

et al. 2017

Front. Plant Sci.

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“…In that research, a total of 407 differential proteins were identified, and several major host proteins responsive to TMV infection/ replication were found. Most of the tobacco proteomics researches were involved in resistance to stress, pests and diseases [18,19]. However, proteomics studies regarding to protein changes during the senescence of tobacco leaves, especially those related to photosynthesis, are scarce.…”

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confidence: 99%

iTRAQ-based quantitative proteomic of tobacco (Nicotiana tabacum L.) identified major host proteins involved in photosystems throughout the aging process

Sun

Tang

et al. 2020

Preprint

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Background: Leaf senescence is one of the most common manifestations in plant senescence and has an important effect on plant photosynthesis. Delaying or shorten the senescence of tobacco leaves is one of the important approaches to improve tobacco breeding. However, the molecular regulatory mechanism of tobacco leaf senescence in response to photosynthesis is still poorly understood. To gain insights into the senescence of tobacco leaves, we integrated analyses including photosynthesis, organelle ultrastructure, and proteome of tobacco leaves during senescence. Results:The photosynthetic rate, intercellular CO 2 concentration, stomatal conductance and transpiration rate showed a downward trend, and the stability of organelle decreased with the senescence of tobacco leaves. Isobaric Tag for Relative Absolute Quantitation (iTRAQ) and Parallel Reaction Monitoring (PRM) were used to analyze the proteins expressed in different periods that were estimate based on photosynthetic physiology and ultramicroscopic observations. A total of 321, 319, 223 differentially expressed proteins (DEPs) were identified from over maturity (OM) vs immature (IM), OM vs well maturity (WM) and WM vs IM, respectively, including 122/199, 124/195 and 125/98 up/down proteins, respectively. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis revealed that the DEPs were significantly enriched in metabolic pathways, biosynthesis of secondary metabolites, microbial metabolism in diverse environments and starch and sucrose metabolism. In addition, the down-regulated proteins were also involved in metabolic pathways, such as carbon sequestration of photosynthetic organisms and photosynthesis. PRM analysis was performed and indicated that iTRAQ is highly reliable in the identification of major proteins involved in tobacco senescence. Conclusions:This study provided important technical references for screening of host photosynthetic proteins during tobacco leaves senescence and established a basis for scientifically understanding of the senescence mechanism of tobacco leaves.

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“…Viral multiplication and movement depend on the properties of host cell, so energy metabolism and enzymes in host cells can influence the defense response (Wang et al 2016b). The ubiquitin proteasome system (UPS) controls the abundance of key regulatory proteins and enzymes taking part in proteolysis and regulates many processes in plants including development, hormone signaling, and plant-pathogen interactions via regulation of immune receptors and immune signaling components (Smalle et al 2004, Trujillo et al 2010, Li et al 2014.…”

Section: Pathwaymentioning

confidence: 99%

“…In addition, we detected some genes encoding heat shock proteins within protein processing in the endoplasmic reticulum pathway. Heatb shock protein 70 functions as a molecular chaperone, which is indispensable for preventing aggregation and assisting re-folding of non-native proteins under stress conditions (Wang et al 2016b). Heat shock proteins are considered to regulate the function of NBS-LRR (Belkhadir et al 2004).…”

Section: Pathwaymentioning

confidence: 99%

The methylation pattern of DNA and complex correlations with gene expressions during TuMV infection in Chinese cabbage

Yu¹,

Gao²,

Yang³

et al. 2019

Biol. plant.

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Chinese cabbage (Brassica rapa L. ssp. pekinensis) is one of the most important economic crops. However, its yield and quality can be severely threatened by Turnip mosaic virus (TuMV). Emerging evidence indicates that epigenetic mechanisms, especially DNA methylation, play an important role in regulating gene expression. Therefore, identification of resistance genes modified by DNA methylation during the virus infection would provide a critical clue for improving disease resistance breeding programs. Here, we present detailed analysis for the correlation of DNA methylation and gene expression involved in several anti-pathogen pathways. We also found that different methylation patterns exist in different methylation sites (CG, CHG, and CHH, where H represents A, G, or T) and genomic regions. Furthermore, we identified disease-resistant genes related to the nucleotide binding site-leucine-rich repeats family, auxin, salicylic acid signaling transduction, cell wall biosynthesis, and protein degradation among the different methylated genes (DMGs) suggesting that these genes may be modified by DNA methylation and work together to activate an immune response. The identified DMGs are a valuable resource for discovering resistance genes. Our study not only provides valuable data for future biotechnology research and epigenetic studies, but also helps to explore how the epigenetic mechanisms modify antiviral pathways.

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iTRAQ protein profile analysis provides integrated insight into mechanisms of tolerance to TMV in tobacco (Nicotiana tabacum)

Cited by 37 publications

References 38 publications

Unraveling the Root Proteome Changes and Its Relationship to Molecular Mechanism Underlying Salt Stress Response in Radish (Raphanus sativus L.)

Unraveling the Root Proteome Changes and Its Relationship to Molecular Mechanism Underlying Salt Stress Response in Radish (Raphanus sativus L.)

iTRAQ-based quantitative proteomic of tobacco (Nicotiana tabacum L.) identified major host proteins involved in photosystems throughout the aging process

The methylation pattern of DNA and complex correlations with gene expressions during TuMV infection in Chinese cabbage

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