iTRAQ-based quantitative proteomic analysis reveals the toxic mechanism of diclofenac sodium on the kidney of broiler chicken

Sun, Chuanxi; Lin, Shuqian; Li, Zhen; Liu, Huazheng; Liu, Yixin; Wang, Keke; Zhu, Tianyi; Li, Guiming; Yin, Bin; Wan, R.

doi:10.1016/j.cbpc.2021.109129

Cited by 7 publications

(5 citation statements)

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“…The precursors of the purine ring were determined using an isotope tracer technique: aspartic acid, glutamine, glycine, CO 2 , and one-carbon unit [ 39 40 ]. The “Glycine, serine, and threonine metabolism” pathway was one of the most significantly enriched pathways in the present study, which was also enriched in a previous study on the nephrotoxicity of DFS on broiler chickens [ 14 ]. In the above two studies, GATM was selected as the target DEP and showed a down-regulation trend, highlighting its special status.…”

Section: Discussionsupporting

confidence: 65%

“…Some studies suggested that the primary cause of the toxic effects of DFS in birds is kidney damage, which in turn blocks the excretion of uric acid [ 12 13 ]. A previous study on the nephrotoxicity of DFS on broiler chickens used isobaric tags for relative and absolute quantification (iTRAQ)-based proteomics [ 14 ]. The present study examined the hepatotoxic mechanism.…”

Section: Introductionmentioning

confidence: 99%

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Hepatotoxic mechanism of diclofenac sodium on broiler chicken revealed by iTRAQ-based proteomics analysis

Sun

Zhu

et al. 2022

J Vet Sci

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Background At the therapeutic doses, diclofenac sodium (DFS) has few toxic side effects on mammals. On the other hand, DFS exhibits potent toxicity against birds and the mechanisms remain ambiguous. Objectives This paper was designed to probe the toxicity of DFS exposure on the hepatic proteome of broiler chickens. Methods Twenty 30-day-old broiler chickens were randomized evenly into two groups (n = 10). DFS was administered orally at 10 mg/kg body weight in group A, while the chickens in group B were perfused with saline as a control. Histopathological observations, serum biochemical examinations, and quantitative real-time polymerase chain reaction were performed to assess the liver injury induced by DFS. Proteomics analysis of the liver samples was conducted using isobaric tags for relative and absolute quantification (iTRAQ) technology. Results Ultimately, 201 differentially expressed proteins (DEPs) were obtained, of which 47 were up regulated, and 154 were down regulated. The Gene Ontology classification and Kyoto Encyclopedia of Genes and Genomes pathway analysis were conducted to screen target DEPs associated with DFS hepatotoxicity. The regulatory relationships between DEPs and signaling pathways were embodied via a protein-protein interaction network. The results showed that the DEPs enriched in multiple pathways, which might be related to the hepatotoxicity of DFS, were “protein processing in endoplasmic reticulum,” “retinol metabolism,” and “glycine, serine, and threonine metabolism.” Conclusions The hepatotoxicity of DFS on broiler chickens might be achieved by inducing the apoptosis of hepatocytes and affecting the metabolism of retinol and purine. The present study could provide molecular insights into the hepatotoxicity of DFS on broiler chickens.

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Section: Discussionsupporting

confidence: 65%

Section: Introductionmentioning

confidence: 99%

Hepatotoxic mechanism of diclofenac sodium on broiler chicken revealed by iTRAQ-based proteomics analysis

Sun

Zhu

et al. 2022

J Vet Sci

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“…Then sarcosine is converted to glycine under the action of DMGDHL1. GATM can catalyze the transfer of guanidine from L-arginine to glycine, which is the rate limiting step of creatine synthesis ( Sandell et al, 2003 ; Sun et al, 2021 ). With alanine as the amino donor, AGXT2 catalyzes the conversion of the intermediate metabolite glyoxylic acid to glycine ( Seppälä et al, 2014 ).…”

Section: Discussionmentioning

confidence: 99%

Salvia miltiorrhiza polysaccharides alleviates florfenicol-induced liver metabolic disorder in chicks by regulating drug and amino acid metabolic signaling pathways

Liu

Liu²,

Fang³

et al. 2022

Poultry Science

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“…Previous research showed that the pathway of "protein processing in endoplasmic reticulum" can have different effects on chickens depending on the conditions they are exposed to. For example, the pathway might be enriched and participate in the apoptotic process in response to toxic substances (Sun et al, 2021) or environmental changes (Srikanth et al, 2019), however, it can also be downregulated in response to the infection by parasites (Li et al, 2019). A complicated process must exist in a broiler's body in response to various conditions.…”

Section: Intestinal Microbiota Comparison In the Cecal Contents And T...mentioning

confidence: 99%

Glucose oxidase as an alternative to antibiotic growth promoters improves the immunity function, antioxidative status, and cecal microbiota environment in white-feathered broilers

et al. 2023

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This study aimed to demonstrate the effects of glucose oxidase (GOD) on broilers as a potential antibiotic substitute. A total of four hundred twenty 1-day-old male Cobb500 broilers were randomly assigned into five dietary treatments, each with six replicates (12 chicks per replicate). The treatments included two control groups (a basal diet and a basal diet with 50 mg/kg aureomycin) and three GOD-additive groups involving three different concentrations of GOD. Analysis after the t-test showed that, on day 21, the feed:gain ratio significantly decreased in the 1,200 U/kg GOD-supplied group (GOD1200) compared to the antibiotic group (Ant). The same effect was also observed in GOD1200 during days 22–42 and in the 600 U/kg GOD-supplied group (GOD600) when compared to the control group (Ctr). The serum tests indicated that, on day 21, the TGF-β cytokine was significantly decreased in both GOD600 and GOD1200 when compared with Ctr. A decrease in malondialdehyde and an increase in superoxide dismutase in GOD1200 were observed, which is similar to the effects seen in Ant. On day 42, the D-lactate and glutathione peroxidase activity changed remarkably in GOD1200 and surpassed Ant. Furthermore, GOD upregulated the expression of the jejunal barrier genes (MUC-2 and ZO-1) in two phases relative to Ctr. In the aureomycin-supplied group, the secretory immunoglobulin A significantly decreased in the jejunum at 42 days. Changes in microbial genera were also discovered in the cecum by sequencing 16S rRNA genes at 42 days. The biomarkers for GOD supplementation were identified as Colidextribacter, Oscillibacter, Flavonifractor, Oscillospira, and Shuttleworthia. Except for Shuttleworthia, all the abovementioned genera were n-butyrate producers known for imparting their various benefits to broilers. The PICRUSt prediction of microbial communities revealed 11 pathways that were enriched in both the control and GOD-supplied groups. GOD1200 accounted for an increased number of metabolic pathways, demonstrating their potential in aiding nutrient absorption and digestion. In conclusion, a diet containing GOD can be beneficial to broiler health, particularly at a GOD concentration of 1,200 U/kg. The improved feed conversion ratio, immunity, antioxidative capacity, and intestinal condition demonstrated that GOD could be a valuable alternative to antibiotics in broiler breeding.

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iTRAQ-based quantitative proteomic analysis reveals the toxic mechanism of diclofenac sodium on the kidney of broiler chicken

Cited by 7 publications

References 64 publications

Hepatotoxic mechanism of diclofenac sodium on broiler chicken revealed by iTRAQ-based proteomics analysis

Hepatotoxic mechanism of diclofenac sodium on broiler chicken revealed by iTRAQ-based proteomics analysis

Salvia miltiorrhiza polysaccharides alleviates florfenicol-induced liver metabolic disorder in chicks by regulating drug and amino acid metabolic signaling pathways

Glucose oxidase as an alternative to antibiotic growth promoters improves the immunity function, antioxidative status, and cecal microbiota environment in white-feathered broilers

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