iTRAQ‐based quantitative proteomic analysis reveals metabolic changes in overwintering
            <i>Scylla paramamosain</i>
            at two different salinities

Zhou, Junming; Li, Na; Wang, Chunlin; Wang, Chunlin; Mu, Changkao

doi:10.1111/are.15221

Cited by 3 publications

(1 citation statement)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The downregulation of LV-ACE under low saintly could be a response to low salt concentration in this group. In contrast to our findings, proteomics analysis exhibited an upregulation in the ACE protein under the low salinity group in the hepatopancreas of mud crab Scylla paramamosain ( 26 ). The ACE enzyme was initially isolated as a ‘hypertensin-converting enzyme’ in 1956 ( 27 ).…”

Section: Discussioncontrasting

confidence: 99%

The role of the renin-angiotensin system (RAS) in salinity adaptation in Pacific white shrimp (Litopenaeus vannamei)

Farhadi

Liu

et al. 2022

Front. Endocrinol.

View full text Add to dashboard Cite

The renin-angiotensin system (RAS) is a hormonal system that plays an important role in the regulation of blood pressure and cardiovascular homeostasis in mammals. In fishes, the RAS pathway participates in osmoregulation and salinity adaptation. However, the role of the RAS pathway in invertebrates, particularly in crustaceans, remains unknown. In this study, four key genes of the RAS pathway (LV-ACE, LV-APN, LV-AT1R, and LV-RR) were cloned, characterized, and their expression levels were detected in the eyestalk, hepatopancreas, and muscle of Litopenaeus vannamei during long-term and short-term low salinity stress. The results showed that LV-ACE, LV-APN, LV-AT1R, and LV-RR encode 666, 936, 175, and 323 amino acids, respectively. Low salinity stress downregulated the expression levels of LV-ACE, LV-APN, LV-AT1R, and LV-RR in L. vannamei, indicating that the RAS pathway was suppressed under low salinity. Moreover, these genes play important roles in the regulation of drinking rate, controlling urine output, blood glucose, and blood pressure, indicating that their downregulation probably affected the homeostasis of shrimps. These findings provide novel insights into the mechanism of salinity adaptation in L. vannamei.

show abstract

Section: Discussioncontrasting

confidence: 99%

The role of the renin-angiotensin system (RAS) in salinity adaptation in Pacific white shrimp (Litopenaeus vannamei)

Farhadi

Liu

et al. 2022

Front. Endocrinol.

View full text Add to dashboard Cite

show abstract

Evidence from transcriptome analysis unravelled the roles of eyestalk in salinity adaptation in Pacific white shrimp (Litopenaeus vannamei)

Farhadi

Liu

et al. 2022

General and Comparative Endocrinology

View full text Add to dashboard Cite

TMT-Based Quantitative Proteomics Reveal the Metabolic Changes Underlying Growth Superiority in a Novel Gymnocypris Hybrid, Gymnocypris przewalskii ♀ × G. eckloni ♂

Dong,

Zhou,

et al. 2024

Fishes

View full text Add to dashboard Cite

Hybrid breeding is an effective approach to generate better varieties and prevent variety degradation. The present study investigated the metabolic changes underlying growth superiority in the novel Gymnocypris hybrid (GH), Gymnocypris przewalskii ♀ (GP) × G. eckloni ♂ (GE). The ranking of survival rate was GH > GE > GP, whereas the ranking of growth rate was GE > GH > GP. A proteomic analysis of G. hybrid and its parents was conducted to elucidate the metabolic changes underlying growth superiority. Identified pathways were primarily associated with amino acid, carbohydrate, energy, lipid, and nucleotide metabolism. These metabolic pathways, which are closely associated with growth, are controlled through regulation of the expression of numerous proteins, including adenosylhomocysteinase, hydroxypyruvate reductase, glutamate-cysteine ligase, L-lactate dehydrogenase, creatine kinase, GDP-L-fucose synthase, pyruvate kinase, fructose-bisphosphate aldolase, carbonic anhydrase, phosphopyruvate hydratase, phosphoglycerate kinase, S-(hydroxymethyl) glutathione dehydrogenase, and AMP deaminase. Real-time PCR assays showed that the level of mRNA expression of differentially expressed genes was positively correlated with growth. Proteins that were differentially expressed in GH exhibited fewer differences from GP and more differences from GE. These data are the first to reveal the molecular mechanism whereby growth is regulated in G. hybrid and its parents at the protein level. The study thus provides important information for genetic breeding and improvement of G. hybrid for aquaculture production.

show abstract

iTRAQ‐based quantitative proteomic analysis reveals metabolic changes in overwintering Scylla paramamosain at two different salinities

Abstract: Scylla paramamosain is a crab species that is widely distributed along the coast of China. This species has been cultivated in China for more than 100 years. S. paramamosain has become the most important cultured crab species in China (

Cited by 3 publications

References 38 publications

The role of the renin-angiotensin system (RAS) in salinity adaptation in Pacific white shrimp (Litopenaeus vannamei)

The role of the renin-angiotensin system (RAS) in salinity adaptation in Pacific white shrimp (Litopenaeus vannamei)

Evidence from transcriptome analysis unravelled the roles of eyestalk in salinity adaptation in Pacific white shrimp (Litopenaeus vannamei)

TMT-Based Quantitative Proteomics Reveal the Metabolic Changes Underlying Growth Superiority in a Novel Gymnocypris Hybrid, Gymnocypris przewalskii ♀ × G. eckloni ♂

Contact Info

Product

Resources

About