ITGB4 as a novel serum diagnosis biomarker and potential therapeutic target for colorectal cancer

Jiang, Xia; Wang, Jia; Wang, Mengyu; Xuan, Mingda; Han, Shuangshuang; Li, Chao; Li, Meng; Sun, Xiao‐Feng; Yu, Weifang; Zhao, Zhenze

doi:10.1002/cam4.4216

Cited by 11 publications

(8 citation statements)

References 40 publications

(40 reference statements)

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“…Additionally, serum ITGB4 was also identified as a novel prospective diagnostic biomarker for CRC by Jiang et al 34,35 Furthermore, ITGB4…”

Section: Discussionmentioning

confidence: 99%

“…ITGB4 has already been confirmed to have a high expression level and was associated with poor prognosis in colorectal cancer, implying ITGB4 could be used as a potential diagnostic and prognostic biomarker for CRC. Additionally, serum ITGB4 was also identified as a novel prospective diagnostic biomarker for CRC by Jiang et al 34,35 Furthermore, ITGB4 was also suggested to be upregulated in both LUAD and LUSC and has potential value in predicting lung cancer prognosis 36 . BRCA2 plays a significant role in cell physiology as a tumor suppressor that promotes DNA replication and DNA double‐strand break repair through homologous recombination 37 .…”

Section: Discussionmentioning

confidence: 99%

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Development of the prognostic value in lung adenocarcinoma based on anoikis‐related genes and initial experimental validation

Guo

Zhao

Liu³

et al. 2023

The Journal of Gene Medicine

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BackgroundLung adenocarcinoma (LUAD) is a highly aggressive cancer in advanced stages and has the highest cancer‐related death across the world. Anoikis has emerged as a specific form of apoptotic cell death that may play a vital role in the formation and development of tumors.MethodsBased on The Cancer Genome Atlas dataset, we developed a novel anoikis‐related genes (ARGs) signature in LUAD and evaluated the differences between low and high‐risk groups in clinical characteristics, expression patterns, immune cell infiltration, and drug sensitivity, etc. According to multivariate Cox regression analysis, the risk score was identified as a significant independent prognostic factor. The possible biological pathways of ARGs' were assessed by Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses. The immune infiltration landscape and risk score of ARGs were analyzed by ESTIMATE and CIBERSORT analysis. A nomogram grounded on six key ARGs and clinicopathological features was provided. Moreover, experiment validation of the expression patterns of six hub ARGs in lung cancer cell lines was conducted.ResultsWe identified 53 survival‐related LUAD anoikis‐related differentially expressed genes and finally six hub anoikis genes (LDHA, SLC2A1, SERPINB5, ITGB4, BRCA2, and PIK3R1) were selected to construct an ARG model. The risk model could efficiently cluster the patients into low‐ and high‐risk groups which could accurately predict clinical outcomes for LUAD patients. There is evidence that the prognostic risk score is a remarkable prognostic factor in determining overall survival. Different immune statuses and drug sensitivity between low‐ and high‐risk groups were explored according to functional analysis. On the basis of risk scores and LUAD clinicopathological features, a novel nomogram was developed. Ultimately, all six key genes except for PIK3R1 were proved to be upregulated in LUAD tissues and cell lines by bioinformatics analysis and experimental validation.ConclusionsThe result of the present study suggest that ARGs could be carcinogenic to LUAD and could be used as an effective stratification factor to customize therapies and forecast the survival rate in LUAD patients.

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“…Additionally, serum ITGB4 was also identified as a novel prospective diagnostic biomarker for CRC by Jiang et al 34,35 Furthermore, ITGB4…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Development of the prognostic value in lung adenocarcinoma based on anoikis‐related genes and initial experimental validation

Guo

Zhao

Liu³

et al. 2023

The Journal of Gene Medicine

View full text Add to dashboard Cite

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“…ITGB4 has been identi ed to be a novel promising serum diagnostic biomarker and a potential therapeutic target for CRC [30][31][32]. However, the association between ITGB4 and CAFs in CRC has not been reported.…”

Section: Discussionmentioning

confidence: 99%

Exosome-transmitted FOSL1 from cancer-associated fibroblasts drives colorectal cancer stemness and chemoresistance through transcriptionally activating ITGB4

Lin

Zhu

2023

Preprint

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Background Cancer-associated fibroblasts (CAFs) have been proved to facilitate colorectal cancer (CRC) development, either with boosting chemo-resistance by communicating with CRC cells in the tumor microenvironment. However, the underlying molecular mechanisms remain largely unclear. Methods Relative expressions of FOSL1 and ITGB4, either with their correlations in CRC tissues were assessed using qRT-PCR analysis. Also, Kaplan-Meier survival analysis was employed for evaluating the prognosis. Identification of CAFs was determined by the detection of specific makers (α-SMA, FAP, and FSP1) using western blot and immunofluorescence staining. Cell proliferation, self-renewal capacity and cell apoptosis were estimated by CCK-8, sphere-formation and flow cytometry assays. Transcriptional regulation of FOSL1 on integrin β4 (ITGB4) was confirmed using ChIP and dual-luciferase reporter assays. Results Increased FOSL1 and ITGB4 in CRC tissues was both positively correlated with poor prognosis of CRC patients. Interestingly, FOSL1 was enriched in the CAFs isolated from CRC stroma, instead of ITGB4. Co-culture CRC cells with CAFs-conditioned medium (CAFs-CM) increased FOSL1 expression, promoted cell proliferation and oxaliplatin resistance, these effects could be blocked by exosome inhibitor (GW4869). Under the treatment of CAFs-derived exosomal FOSL1, CRC cells exhibited stronger proliferative ability and oxaliplatin resistance. Conclusion Our results uncovered that CAFs-derived exosomes could transfer FOSL1 to CRC cells, thereby promoting CRC cell proliferation, stemness, and oxaliplatin resistance by transcriptionally activating ITGB4.

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“…The PPyNW-modified biosensor showed significantly enhanced sensitivity, conductivity, and biocompatibility. In addition, serum angiopoietin, MST1/STK4, S100A9 TIMP1, ITGB4, Cyr61, and CXCL-8 in the blood have also been proven to have potential uses as biomarkers in the diagnosis and detection of colorectal cancer, and they could be employed in electrochemical sensor detection after further verification [ 166 , 167 , 168 , 169 , 170 ].…”

Section: Ppy-based Biosensors For Crc Biomarker Detectionmentioning

confidence: 99%

Application of Polypyrrole-Based Electrochemical Biosensor for the Early Diagnosis of Colorectal Cancer

et al. 2023

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Although colorectal cancer (CRC) is easy to treat surgically and can be combined with postoperative chemotherapy, its five-year survival rate is still not optimistic. Therefore, developing sensitive, efficient, and compliant detection technology is essential to diagnose CRC at an early stage, providing more opportunities for effective treatment and intervention. Currently, the widely used clinical CRC detection methods include endoscopy, stool examination, imaging modalities, and tumor biomarker detection; among them, blood biomarkers, a noninvasive strategy for CRC screening, have shown significant potential for early diagnosis, prediction, prognosis, and staging of cancer. As shown by recent studies, electrochemical biosensors have attracted extensive attention for the detection of blood biomarkers because of their advantages of being cost-effective and having sound sensitivity, good versatility, high selectivity, and a fast response. Among these, nano-conductive polymer materials, especially the conductive polymer polypyrrole (PPy), have been broadly applied to improve sensing performance due to their excellent electrical properties and the flexibility of their surface properties, as well as their easy preparation and functionalization and good biocompatibility. This review mainly discusses the characteristics of PPy-based biosensors, their synthetic methods, and their application for the detection of CRC biomarkers. Finally, the opportunities and challenges related to the use of PPy-based sensors for diagnosing CRC are also discussed.

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ITGB4 as a novel serum diagnosis biomarker and potential therapeutic target for colorectal cancer

Abstract: This is an open access article under the terms of the Creat ive Commo ns Attri bution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

Cited by 11 publications

References 40 publications

Development of the prognostic value in lung adenocarcinoma based on anoikis‐related genes and initial experimental validation

Development of the prognostic value in lung adenocarcinoma based on anoikis‐related genes and initial experimental validation

Exosome-transmitted FOSL1 from cancer-associated fibroblasts drives colorectal cancer stemness and chemoresistance through transcriptionally activating ITGB4

Application of Polypyrrole-Based Electrochemical Biosensor for the Early Diagnosis of Colorectal Cancer

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