It's Electric: When Technology Gives a Boost to Stem Cell Science

Lee, Abraham P.; Aghaamoo, Mohammad; Adams, Tayloria N.G.; Flanagan, Lisa A.

doi:10.1007/s40778-018-0124-x

Cited by 14 publications

(11 citation statements)

References 77 publications

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“…NSPCs are distinguished from differentiated neurons and astrocytes and prospectively sorted from neurons by membrane capacitance using DEP ( Flanagan et al., 2008 , Prieto et al., 2012 ). Membrane capacitance defines and enables the enrichment of undifferentiated and differentiated cells in the hematopoietic stem cell, mesenchymal stem cell (MSC)/adipose-derived stem cell, and embryonic stem cell lineages, indicating the relevance of biophysical properties to fate across multiple stem cell types (for a recent review see Lee et al., 2018 ).…”

Section: Introductionmentioning

confidence: 99%

Cell Surface N-Glycans Influence Electrophysiological Properties and Fate Potential of Neural Stem Cells

et al. 2018

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SummaryUnderstanding the cellular properties controlling neural stem and progenitor cell (NSPC) fate choice will improve their therapeutic potential. The electrophysiological measure whole-cell membrane capacitance reflects fate bias in the neural lineage but the cellular properties underlying membrane capacitance are poorly understood. We tested the hypothesis that cell surface carbohydrates contribute to NSPC membrane capacitance and fate. We found NSPCs differing in fate potential express distinct patterns of glycosylation enzymes. Screening several glycosylation pathways revealed that the one forming highly branched N-glycans differs between neurogenic and astrogenic populations of cells in vitro and in vivo. Enhancing highly branched N-glycans on NSPCs significantly increases membrane capacitance and leads to the generation of more astrocytes at the expense of neurons with no effect on cell size, viability, or proliferation. These data identify the N-glycan branching pathway as a significant regulator of membrane capacitance and fate choice in the neural lineage.

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Section: Introductionmentioning

confidence: 99%

Cell Surface N-Glycans Influence Electrophysiological Properties and Fate Potential of Neural Stem Cells

et al. 2018

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“…The cell patterning capabilities of EIS combined with DEP can be employed to build complex 3D microenvironments that represent in vivo cancer conditions. Another aspect of DEP is its implementation to continuously sort a variety of cells [ 81 , 101 , 102 , 103 , 104 ], thus an exciting possibility is to sort CSCs from non-CSCs prior to impedance monitoring. These techniques can also be used for drug optimization and monitoring as well as to understand the molecular and environmental driving forces involved in plasticity.…”

Section: Future Trends In Monitoring Cancer Cell Dynamicsmentioning

confidence: 99%

Electrical Impedance Spectroscopy for Monitoring Chemoresistance of Cancer Cells

et al. 2020

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Electrical impedance spectroscopy (EIS) is an electrokinetic method that allows for the characterization of intrinsic dielectric properties of cells. EIS has emerged in the last decade as a promising method for the characterization of cancerous cells, providing information on inductance, capacitance, and impedance of cells. The individual cell behavior can be quantified using its characteristic phase angle, amplitude, and frequency measurements obtained by fitting the input frequency-dependent cellular response to a resistor–capacitor circuit model. These electrical properties will provide important information about unique biomarkers related to the behavior of these cancerous cells, especially monitoring their chemoresistivity and sensitivity to chemotherapeutics. There are currently few methods to assess drug resistant cancer cells, and therefore it is difficult to identify and eliminate drug-resistant cancer cells found in static and metastatic tumors. Establishing techniques for the real-time monitoring of changes in cancer cell phenotypes is, therefore, important for understanding cancer cell dynamics and their plastic properties. EIS can be used to monitor these changes. In this review, we will cover the theory behind EIS, other impedance techniques, and how EIS can be used to monitor cell behavior and phenotype changes within cancerous cells.

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“…It has been demonstrated that cell membrane capacitance, a type of cell dielectric parameter, can be used for the real-time, label-free, and contact-free monitoring of stem cell differentiation [13]. Additionally, whole cell membrane capacitance serving as a label-free biomarker can be employed to monitor stem cell fate and differentiation, thereby opening new avenues for regulating stem cell fate and continued progress as well as enhancing our understanding of basic stem cell biology [14]. Most importantly, the label-free and non-invasive isolation and enrichment of the circulating tumor cells (CTCs) derived from clinical samples is another application of the extracted cell dielectric properties, which will give valuable prognostic guides for treating malignant diseases and detecting the metastasis and deterioration of tumors [15].…”

Section: Introductionmentioning

confidence: 99%

Determination of Dielectric Properties of Cells using AC Electrokinetic-based Microfluidic Platform: A Review of Recent Advances

Yang

Wang

et al. 2020

Micromachines

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Cell dielectric properties, a type of intrinsic property of cells, can be used as electrophysiological biomarkers that offer a label-free way to characterize cell phenotypes and states, purify clinical samples, and identify target cancer cells. Here, we present a review of the determination of cell dielectric properties using alternating current (AC) electrokinetic-based microfluidic mechanisms, including electro-rotation (ROT) and dielectrophoresis (DEP). The review covers theoretically how ROT and DEP work to extract cell dielectric properties. We also dive into the details of differently structured ROT chips, followed by a discussion on the determination of cell dielectric properties and the use of these properties in bio-related applications. Additionally, the review offers a look at the future challenges facing the AC electrokinetic-based microfluidic platform in terms of acquiring cell dielectric parameters. Our conclusion is that this platform will bring biomedical and bioengineering sciences to the next level and ultimately achieve the shift from lab-oriented research to real-world applications.

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It's Electric: When Technology Gives a Boost to Stem Cell Science

Cited by 14 publications

References 77 publications

Cell Surface N-Glycans Influence Electrophysiological Properties and Fate Potential of Neural Stem Cells

Cell Surface N-Glycans Influence Electrophysiological Properties and Fate Potential of Neural Stem Cells

Electrical Impedance Spectroscopy for Monitoring Chemoresistance of Cancer Cells

Determination of Dielectric Properties of Cells using AC Electrokinetic-based Microfluidic Platform: A Review of Recent Advances

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