Ist die Pikrinsäurereaktion für die Bestimmung des Blutzuckers in der Praxis brauchbar?

Kaufmann, E.

doi:10.1007/bf02665652

Cited by 3 publications

(1 citation statement)

References 13 publications

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“…warming, an immediate blood red-coloured reduction Jaffe reported in 1886 that a red colour formed at P^duct of picric acid formed Other investigators had room temperature when creatinine was reacted with also reported that a red colour formed upon the ν ν ο Ά + 11 r ττ ΛΙ\ τν/τ Λ-f Α τ Λ" application of heat (2,4,12,(15)(16)(17)(18)(19) the cold, glucose gave a positive reaction with alkaline procedure for the measurement of creatinine in biof · , logical samples. However, dependent upon the reac-p ; crate -At room *****»?…”

Section: Introductionmentioning

confidence: 99%

Effect of Glucose upon Alkaline Picrate: A Jaffé Interference

Viraraghavan¹,

Blass²

1990

Clinical Chemistry and Laboratory Medicine

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Summary:The reactivity of glucose in aqueous alkaline picrate was investigated by spectrophotometry and polarography at 25 °C in 0.51 mol/1 sodium hydroxide. Thin-layer chromatography and infrared spectroscopy studies have conclusively identified the presence of picramic acid in 5:1 and 10:1 glucose picrate test solutions incubated at 25 °C.The polarographic data of an alkaline picrate blank with a concentration of 0.284 mmol/1, show three welldefined nitro group reduction waves with approximate half-wave potentials of -0.62 V, -0.78 V, and -0.93 V and a fourth broad wave appearing near -1.31 V versus a saturated calomel electrode. The addition of glucose to alkaline picrate resulted in a decreased diffusion current for reduction waves 1-3, with little change in reduction wave 4. The reactivity of test solutions containing glucose: picrate in 1:1, 2:1, 5:1 and 10:1 molar ratios was investigated at varied time intervals between 10 and 180 minutes.The absorption spectra of a 10:1 glucose: picrate solution shifted from 356 nm to 375 nm and a broad tailing shoulder absorbance formed in the 450-600 nm region. An orange coloured minor product, separated by thin-layer chromatography, was observed to fluoresce. The maximum excitation and emission wavelengths were 318 nm and 545 nm, respectively. A major, red-coloured product was isolated and identified as picramic acid by infrared spectroscopy. For 10:1 glucose: picrate test solutions incubated at 25 °C, picramic acid formed within 10 minutes. Within the first minute, the colour was observed to change from yellow to orange and then to red.

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Section: Introductionmentioning

confidence: 99%

Effect of Glucose upon Alkaline Picrate: A Jaffé Interference

Viraraghavan¹,

Blass²

1990

Clinical Chemistry and Laboratory Medicine

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Zum Kohlenhydratstoffwechsel und Kohlenhydratbedarf unter der modernen Narkose

Schostok

1952

Arch. f. klin. Chir

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Die Frage nach der Art des Ersatzes der ureter der Operation verlorengegangenen Flfissigkeitsmengen war bis vor kurzem yore Mineralstoffwechsel aus dahingehend beantworget, dal3 man die Rehydration des Org{mismus und vor allem die Auffiillung der zirkulierenden Blutflfissigkeit mit physiologischer Kochsa]zlSsung und ihren Modifikationen vornahm und als zwingend ansah.Schon friiher hab~n aber einige Autoren (KAVSCH, Lo~n, T~zI~ss) auf die Ge/ahren der NaCl-In/usionen mit der Retention yon Wasser im Gewebe, demAuftreten yon LungenSdemen und mit pathologisch~anatomisch nachweisbaren Sch~digungen des Herzmuskels (Ho]~ssLI, RSSSLE) hingewiesen. ST~AV-B bezeieh-

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Das Verhalten der Restreduktion und des Glutathion im Blut der Schwangeren

Siedentopf

Hertel

1938

Arch. Gynak.

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Ist die Pikrinsäurereaktion für die Bestimmung des Blutzuckers in der Praxis brauchbar?

Cited by 3 publications

References 13 publications

Effect of Glucose upon Alkaline Picrate: A Jaffé Interference

Effect of Glucose upon Alkaline Picrate: A Jaffé Interference

Zum Kohlenhydratstoffwechsel und Kohlenhydratbedarf unter der modernen Narkose

Das Verhalten der Restreduktion und des Glutathion im Blut der Schwangeren

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