“…Molecular markers based on polymerase chain reaction (PCR) are widely used since they require a relatively small amount of DNA and are technically simple and not affected by environmental, pleiotropic and epistatic effects (Mondini et al, 2009). Among the variable molecular markers, ISSRs have been widely used to study plant genetic variations such as gene tagging (Gopalakrishna and Joshi-Saha, 2007), genetic diversity (Abirami et al, 2018), fingerprinting (Bhagyawant and Srivastava, 2008) and marker-assisted selection (Vijayan et al, 2006). The principle of ISSR is similar to RAPD in which random primers are used.…”