ISSR-based analysis of genetic diversity among sorghum landraces growing in some parts of Saudi Arabia and Yemen

Abstract

Inter simple sequence repeats (ISSR) analysis was used to determine the genetic diversity among 15 genotypes of sorghum [Sorghum bicolor (L.) Moench] growing in some parts of Saudi Arabia and Yemen. A total of 92 alleles were amplified, with an average of 13 ISSR alleles per primer. Cluster analysis divided the 15 genotypes into two main groups. Group A consisted of five genotypes with white grains from Jazan and Abha with a similarity coefficient range of 0.527 to 0.818. Group B was comprised of 10 genotypes; two genotypes from Al-Qassim were clearly delimited from the remaining eight samples with a coefficient range from 0.709 to 0.490. The eight genotypes were divided into two clusters; one was comprised of landraces with dark grains from Abha in Saudi Arabia and Ab in Yemen, with a similarity coefficient range between 0.563 and 0.781, and the other cluster was differentiated into three white-colored-grain genotypes and one colored-grain genotype; all samples from North Yemen had a similarity coefficient range from 0.454 to 0.800. The current results encourage further collection and authentication of sorghum landraces in the gene banks of Saudi Arabia.

Exploring the correlation between salt tolerance and yield: research advances and perspectives for salt-tolerant forage sorghum selection and genetic improvement

“…For effective sorghum breeding, advanced methods in molecular marker-assisted selection should be exploited. So far, SSR (Shehzad et al 2009 ), SNP (Luo et al 2016 ), RAPD (Akhare et al 2008 ), and ISSR (Basahi 2015 ) markers have been developed for sorghum. From these markers, SNPs have been the most intensively studied in sorghum as far as salt tolerance and yield are concerned.…”

Evaluation of the Genetic Relationships of Some Endangered Tunisian Peas Adapted to Arid Regions and Turkish Accessions Revealed by Inter Simple Sequence Repeat (ISSR) Markers

“…While, they were lower to those obtained in other studies with ISSRs, such as those of Pandian et al [43] in sorghum "Sorghumsp. ", Basahi et al [44] and Subhojit et al [45] in faba bean "Vicia faba" and lentil "Lens culinaris", and Dagnew et al [46] in Phaseolus vulgaris. These results showed that the greatest degree of polymorphism among the 24 accessions proved the capacity of the ISSR marker to study the evaluation of genetic variability between close genotypes of identical or different geographic origin.…”

The Promise of Molecular and Genomic Techniques for Biodiversity Research and DNA Barcoding of the Arabian Peninsula Flora

“…Another study suggested the use of ISSR markers to detect phenotypic variation amongst 15 genotypes of Sorghum landrace grown in Saudi Arabia and Yemen. In this case, eight genotypes of Sorghum bicolor were successfully differentiated into two clusters, one with dark grains and the other with white grains (Basahi, 2015). Recently, Al-Ameri et al (2016) used ISSR markers for the early sex determination of date palms in Saudi Arabia.…”