ISPpy1, a novel mobile element of the ancient Psychrobacter maritimus permafrost strain: Translocations in Escherichia coli K-12 cells and formation of composite transposons

Petrova, Mayya; Gorlenko, Zh. M.; Scherbatova, N. A.; Mindlin, S. Z.

doi:10.1134/s1022795412020111

Cited by 3 publications

(10 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The E. coli K-12 strains used were JF238 (prototroph, gyrA91) and the Rif R derivative of UB5201 (pro met recA56 gyrA rif-r) (Kholodii et al, 2000). The recombinant plasmids pBR325-80.2 (Sm R Tc R Cm R ) and pBR325-Ppy1 (Tc R Cm R ), which are derivatives of pBR325 (Petrova et al, 2012) containing the transposons Tn5080a and ISPpy1 inserted into the ampicillinresistance gene (bla), respectively, were used in transposition experiments. Plasmid pBR325 (Ap R Tc R Cm R ) was used as a control.…”

Section: Methodsmentioning

confidence: 99%

“…We used two JF238 derivatives carrying the independent Tn5080a chromosome insertions JF147 and JF148 (Petrova et al, 2012). The plasmid pGEM5Zf(-) was introduced into these strains via transformation.…”

Section: Methodsmentioning

confidence: 99%

“…To isolate independent Tn5080a insertions, only one or two recombinant plasmids were chosen from each experiment. In addition to derivatives of pGEM-5Zf(-), insertions of ISPpy1 and Tn5080a into plasmid pBR325, which were obtained in our previous work, were also analysed (Petrova et al, 2012). In both plasmid sets, only insertions into the bla gene were investigated.…”

Section: Methodsmentioning

confidence: 99%

“…This element was found on the plasmid pKLH80 in the ancient bacterial strain Psychrobacter maritimus MR29-12 (previously designated P. psychrophilus) isolated from a 15 000-40 000 year old permafrost sample Petrova et al, 2009). Intriguingly, ISPpy1 exhibited pronounced transposition activity in cells of heterologous hosts, such as Acinetobacter calcoaceticus and E. coli (Petrova et al, 2009(Petrova et al, , 2012. It was demonstrated that, in both species, ISPpy1 can generate novel composite transposons (designated Tn5080 and Tn5080a, Fig.…”

Section: Introductionmentioning

confidence: 99%

“…1b) flanked by two copies of ISPpy1 and containing streptomycin-and tetracyclineresistance genes derived from pKLH80. It was also shown that the composite transposon Tn5080a translocates efficiently into different target plasmids and into the E. coli chromosome (Petrova et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

A new subgroup of the IS3 family and properties of its representative member ISPpy1

et al. 2013

View full text Add to dashboard Cite

Institute of Molecular Genetics, Russian Academy of Sciences, Kurchatov sq. 2, Moscow 123182, RussiaRecently, we described a novel insertion element, ISPpy1, isolated from a permafrost strain of Psychrobacter maritimus. In this work, we demonstrated that ISPpy1 is a member of a novel subgroup of the IS3 family of insertion sequences (ISs) that was not identified and characterized previously. IS elements of this subgroup termed the ISPpy1 subgroup are broadly distributed among different taxa of Eubacteria, including Geobacteraceae, Chlorobiaceae, Desulfobacteraceae, Methylobacteriaceae, Nitrosomonadaceae and Cyanobacteria. While displaying characteristic features of the IS3-family elements, ISPpy1 subgroup elements exhibit some unusual features. In particular, most of them have longer terminal repeats with unconventional ends and frameshifting box with an atypical organization, and, unlike many other IS3-family elements, do not exhibit any distinct IS specificity. We studied the transposition and mutagenic properties of a representative member of this subgroup, ISPpy1 and showed that in contrast to the original P. maritimus host, in a heterologous host, Escherichia coli K-12, it is able to translocate with extremely high efficiency into the chromosome, either by itself or as a part of a composite transposon containing two ISPpy1 copies. The majority of transposants carry multiple chromosomal copies (up to 12) of ISPpy1. It was discovered that ISPpy1 is characterized by a marked mutagenic activity in E. coli: its chromosomal insertions generate various types of mutations, including auxotrophic, pleiotropic and rifampicin-resistance mutations. The distribution of IS elements of the novel subgroup among different bacteria, their role in the formation of composite transposons and the horizontal transfer of genes are examined and discussed.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%