Effect of stress on emergence of antibiotic-tolerant Escherichia coli cells

Лойко, Н. Г.; Kozlova, A. N.; Nikolaev, Yu. A.; Gaponov, A. M.; Tutelyan, A.V.; Эль-Регистан, Г. И.

doi:10.1134/s0026261715050148

Cited by 15 publications

(2 citation statements)

References 32 publications

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“…Another strategy for combating hvKp can involve targeting changes in the antibiotic sensitivity of the pathogen. Recent studies on dormant forms of opportunistic pathogenic bacteria (persisters) capable of surviving antibiotic therapy due to antibiotic tolerance [27,84] have confirmed that such cells can be a new target in the fight against antibiotic resistance [134]. Antibiotic-resistant persistent forms are often found among nosocomial strains of pathogens [134], including K. pneumoniae [1].…”

Section: Directions For Controlling the Spread Of Infection Caused By...mentioning

confidence: 99%

Molecular Epidemiology of Hypervirulent K. pneumoniae and Problems of Health-Care Associated Infections

et al. 2022

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The review describes virulence factors of hypervirulent K. pneumoniae (hvKp) including genes determining its virulence and discusses their role in the development of health-care associated infections. The contribution of individual virulence factors and their combination to the development of the hypervirulence and the prospects of using these factors as biomarkers and therapeutic targets are described. Virulence factors of hvKp and “classical” K. pneumoniae strains (cKp) with no hypervirulence genes were compared. The mechanisms of biofilm formation by hvKp and high incidence of its antibiotic resistance are of particular importance for in health care institutions. Therefore, the development of methods for hvKp identification allowing early prevention of severe hvKp infection and novel approaches to abrogate its spreading are new challenges for epidemiology, infection diseases, and critical care medicine. New technologies including bacteriological and molecular studies make it possible to develop innovative strategies to diagnose and treat infection caused by hvKp. These include monitoring of both genetic biomarkers of hvKp and resistance plasmid that carry of virulence genes and antibiotic resistance genes, creation of immunological agents for the prevention and therapy of hvKp (vaccines, monoclonal antibodies) as well as personalized hvKp-specific phage therapies and pharmaceuticals enhancing the effect of antibiotics. A variety of approaches can reliably prepare our medicine for a new challenge: spreading of life-threatening health-care associated infections caused by antibiotic-resistant hvKp strains.

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Section: Directions For Controlling the Spread Of Infection Caused By...mentioning

confidence: 99%

Molecular Epidemiology of Hypervirulent K. pneumoniae and Problems of Health-Care Associated Infections

et al. 2022

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“…Third, their interaction with each other (Dps-Dps) and with DNA (DNA-Dps) produces a unique structure, a biocrystallized nucleoid, in which DNA, RNA, and the nucleoid proteins are protected from the relevant depolymerases and external stress agents [ 36 ]. Moreover, a stationary bacterial population contains a subpopulation of persister cells (~1%), which are formed in the course of cell differentiation [ 37 , 38 ] and, according to our hypothesis [ 39 , 40 ], are precursors of cyst-like anabiotic dormant forms (DF). The transition of a growing population to the stationary phase is always associated with the critical concentration of the density autoregulators of one out of two types: QS system autoinducers [ 41 ] or d 1 factors (alkylresorcinols, etc.)…”

Section: Discussionmentioning

confidence: 99%

Ways of Long-Term Survival of Hydrocarbon-Oxidizing Bacteria in a New Biocomposite Material—Silanol-Humate Gel

et al. 2023

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Immobilized bacterial cells are presently widely used in the development of bacterial preparations for the bioremediation of contaminated environmental objects. Oil hydrocarbons are among the most abundant pollutants. We have previously described a new biocomposite material containing hydrocarbon-oxidizing bacteria (HOB) embedded in silanol-humate gels (SHG) based on humates and aminopropyltriethoxysilane (APTES); high viable cell titer was maintained in this material for at least 12 months. The goal of the work was to describe the ways of long-term HOB survival in SHG and the relevant morphotypes using the techniques of microbiology, instrumental analytical chemistry and biochemistry, and electron microscopy. Bacteria surviving in SHG were characterized by: (1) capacity for rapid reactivation (growth and hydrocarbon oxidation) in fresh medium; (2) ability to synthesize surface-active compounds, which was not observed in the cultures stored without SHG); (3) elevated stress resistance (ability to grow at high Cu2+ and NaCl concentrations); (4) physiological heterogeneity of the populations, which contained the stationary hypometabolic cells, cystlike anabiotic dormant forms (DF), and ultrasmall cells; (5) occurrence of piles in many cells, which were probably used to exchange genetic material; (6) modification of the phase variants spectrum in the population growing after long-term storage in SHG; and (7) oxidation of ethanol and acetate by HOB populations stored in SHG. The combination of the physiological and cytomorphological properties of the cells surviving in SHG for long periods may indicate a new type of long-term bacterial survival, i.e., in a hypometabolic state.

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Stress-Tolerant Dormant Bacterial Forms: Biological and Ultrastructural Properties of Moraxella catarrhalis and Kocuria rhizophila

Manzenyuk,

Suzina,

Nikolaev

et al. 2024

Bull Exp Biol Med

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Effect of stress on emergence of antibiotic-tolerant Escherichia coli cells

Cited by 15 publications

References 32 publications

Molecular Epidemiology of Hypervirulent K. pneumoniae and Problems of Health-Care Associated Infections

Molecular Epidemiology of Hypervirulent K. pneumoniae and Problems of Health-Care Associated Infections

Ways of Long-Term Survival of Hydrocarbon-Oxidizing Bacteria in a New Biocomposite Material—Silanol-Humate Gel

Stress-Tolerant Dormant Bacterial Forms: Biological and Ultrastructural Properties of Moraxella catarrhalis and Kocuria rhizophila

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