Isotype responses of infected, virus-vaccinated and peptide-vaccinated cattle to foot-and-mouth disease virus

Mulcahy, Grace; Gale, C; Robertson, Peter; Iyisan, Selma; DiMarchi, Richard D.; Doel, T. R.

doi:10.1016/0264-410x(90)90054-p

Cited by 75 publications

(41 citation statements)

References 13 publications

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“…Of these anti-FMDV colonies, around 44 % were of the IgG1 isotype and 56 % were of the IgG2 isotype, and some 13 % of these specific colonies, from both isotype groups, neutralized virus in vitro. Differences in the ratios of IgG1 and IgG2 antibodies following the immunization of cattle with either synthetic peptide or whole virus have previously been reported (Mulcahy et al, 1990). Thus a predominance of IgG1 antibodies over IgG2 antibodies was seen in animals receiving infectious or inactivated virus, whereas animals immunized with synthetic peptide produced significantly less IgG1 in comparison to IgG2.…”

Section: Discussionmentioning

confidence: 72%

“…Functional activity and isotype of an antibody are known to be interrelated (McGuire et al, 1979 ;Sellei, 1984 ;PlumasMarty et al, 1993 ;Xu et al, 1994 ;Spira et al, 1996), and the class or subclass induced by a particular antigen is correlated with the appropriate protective mechanism (Mulcahy et al, 1990). Having ascertained the reproducibility of this hybridoma methodology, ten FMDV-specific colonies, five of the IgG1 isotype and five of the IgG2 isotype, were selected for further characterization.…”

Section: Discussionmentioning

confidence: 99%

“…reduction of the ID &! virus titre compared to that observed in the absence of antibody as described by Mulcahy et al (1990). quence of the complete capsid coding region was determined for each BMAb selected virus mutant.…”

Section: Rna Extraction and Sequencing Of Bmab Selected Virus Mutantsmentioning

confidence: 99%

“…Beside their value for defining antigenic sites and investigating the relative importance of individual FMDV epitopes in a natural host, such antibodies might also be used to explore more fully the humoral role of disease intervention. Not excluding the roles of different isotypes which may or may not vary in accordance with their protective ability (Mulcahy et al, 1990) such antibodies may offer a route to a clearer understanding of other functionally important cell processes, such as opsonization and phagocytosis, which may ultimately lead to host protection (McCullough et al, 1988).…”

Section: Introductionmentioning

confidence: 99%

“…This was carried out as described by Mulcahy et al (1990). Test antibody was deemed protective if the reduction in virus titre was 1n5.…”

Section: Rna Extraction and Sequencing Of Bmab Selected Virus Mutantsmentioning

confidence: 99%

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Monoclonal antibodies, against O1 serotype foot-and-mouth disease virus, from a natural bovine host, recognize similar antigenic features to those defined by the mouse.

Barnett

Samuel

Püllen

et al. 1998

Journal of General Virology

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Eight neutralizing and two non-neutralizing antifoot-and-mouth disease virus (FMDV) bovine IgG1 and IgG2 monoclonal antibodies (BMAbs) recognize conformationally dependent epitopes. The majority of those shown to neutralize virus passively protected mice from virus challenge, regardless of isotype. Well-characterized anti-FMDV mouse MAbs, representing five independent neutralizing epitopes on O 1 serotype virus, were examined with each of the ten BMAbs in a competition-based ELISA. Five of the neutralizing BMAbs (C48, C65, C74, C83 and MH6) were shown to be directed against epitopes on, or in close proximity to, that previously defined as neutralizing antigenic site 2. Another neutralizing BMAb, MH5, bound to an epitope on, or in close proximity to antigenic site 3. Two neutralizing BMAbs (C2 and C96) simultaneously

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Section: Discussionmentioning

confidence: 72%

Section: Discussionmentioning

confidence: 99%

Section: Rna Extraction and Sequencing Of Bmab Selected Virus Mutantsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…This was carried out as described by Mulcahy et al (1990). Test antibody was deemed protective if the reduction in virus titre was 1n5.…”

Section: Rna Extraction and Sequencing Of Bmab Selected Virus Mutantsmentioning

confidence: 99%

See 3 more Smart Citations

Monoclonal antibodies, against O1 serotype foot-and-mouth disease virus, from a natural bovine host, recognize similar antigenic features to those defined by the mouse.

Barnett

Samuel

Püllen

et al. 1998

Journal of General Virology

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Development of Replication-Defective Adenovirus Serotype 5 Containing the Capsid and 3C Protease Coding Regions of Foot-and-Mouth Disease Virus as a Vaccine Candidate

1999

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A recombinant replication-defective human adenovirus serotype 5 vector containing FMDV capsid, P1-2A, and viral 3C protease coding regions was constructed. Two viral clones were isolated, Ad5-P12X3CWT, containing the wild-type (WT) 3C protease that processes capsid polyprotein precursor into mature capsid proteins, and Ad5-P12X3CMUT, containing a point mutation in the protease coding region that inhibits processing. In 293 cells infected with either virus, synthesis of the FMDV capsid polyprotein precursor occurred, but processing of the polyprotein into structural proteins VP0, VP3, and VP1 occurred only in 3CWT virus-infected cells. Immunoprecipitation with monospecific and monoclonal antibodies indicates possible higher order structure formation in Ad5-P12X3CWT virus-infected cells. The viruses were used to elicit immune responses in mice inoculated intramuscularly (im). Only virus containing the 3CWT elicited a neutralizing antibody response. After boosting, this neutralizing antibody response increased. Swine inoculated im with Ad5-P12X3CWT virus developed a neutralizing antibody response and were either completely or partially protected from contact challenge with an animal directly inoculated with virulent FMDV. This adenovirus vector may be an efficient system for the delivery of FMDV cDNA into animals, leading to a high level of neutralizing antibody production and protection from FMDV challenge.

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Natural and Vaccine Induced Immunity to FMD

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Current Topics in Microbiology and Immunology

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Isotype responses of infected, virus-vaccinated and peptide-vaccinated cattle to foot-and-mouth disease virus

Cited by 75 publications

References 13 publications

Monoclonal antibodies, against O1 serotype foot-and-mouth disease virus, from a natural bovine host, recognize similar antigenic features to those defined by the mouse.

Monoclonal antibodies, against O1 serotype foot-and-mouth disease virus, from a natural bovine host, recognize similar antigenic features to those defined by the mouse.

Development of Replication-Defective Adenovirus Serotype 5 Containing the Capsid and 3C Protease Coding Regions of Foot-and-Mouth Disease Virus as a Vaccine Candidate

Natural and Vaccine Induced Immunity to FMD

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