Isotropic micropatterned silica coatings on zirconia induce guided cell growth for dental implants

Peláez-Vargas, Alejandro; Gallego‐Perez, Daniel; Magallanes-Perdomo, M.; Fernandes, Maria Helena; Hansford, Derek J.; Aza, Antonio H. De; Pena, P.; Monteiro, Fernando J.

doi:10.1016/j.dental.2011.02.014

Cited by 50 publications

(56 citation statements)

References 69 publications

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“…This is defined as an inflammatory disease that induces the loss of supporting bone in the tissues surrounding a functional implant [7] and it represents the most common reason for implant failure. Hence, in order to obtain sufficient integration of dental implant, it is necessary both, to inhibit biofilm formation and to improve the adhesion between metal implant and peri-implant tissues [8]. Functionalization of titanium implant with bioactive molecules could thus be a pertinent solution to enhance integration of titanium [9][10][11].…”

Section: Introductionmentioning

confidence: 99%

Relevant insight of surface characterization techniques to study covalent grafting of a biopolymer to titanium implant and its acidic resistance

D’Almeida

Amalric²,

Brunon³

et al. 2015

Applied Surface Science

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Section: Introductionmentioning

confidence: 99%

Relevant insight of surface characterization techniques to study covalent grafting of a biopolymer to titanium implant and its acidic resistance

D’Almeida

Amalric²,

Brunon³

et al. 2015

Applied Surface Science

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“…Silica coatings were produced via a sol–gel method described elsewhere [11, 23, 24]. Briefly, tetraethylorthosilicate (TEOS, Aldrich, USA) and methyltriethoxysilane (MTES, Aldrich, USA) were used as silicate precursors in a single stage process with acid catalysis.…”

Section: Methodsmentioning

confidence: 99%

Modulation of human dermal microvascular endothelial cell and human gingival fibroblast behavior by micropatterned silica coating surfaces for zirconia dental implant applications

Laranjeira

Carvalho

Peláez-Vargas

et al. 2014

Science and Technology of Advanced Materials

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Dental ceramic implants have shown superior esthetic behavior and the absence of induced allergic disorders when compared to titanium implants. Zirconia may become a potential candidate to be used as an alternative to titanium dental implants if surface modifications are introduced. In this work, bioactive micropatterned silica coatings were produced on zirconia substrates, using a combined methodology of sol–gel processing and soft lithography. The aim of the work was to compare the in vitro behavior of human gingival fibroblasts (HGFs) and human dermal microvascular endothelial cells (HDMECs) on three types of silica-coated zirconia surfaces: flat and micropatterned (with pillars and with parallel grooves). Our results showed that cells had a higher metabolic activity (HGF, HDMEC) and increased gene expression levels of fibroblast-specific protein-1 (FSP-1) and collagen type I (COL I) on surfaces with pillars. Nevertheless, parallel grooved surfaces were able to guide cell growth. Even capillary tube-like networks of HDMEC were oriented according to the surface geometry. Zirconia and silica with different topographies have shown to be blood compatible and silica coating reduced bacteria adhesion. All together, the results indicated that microstructured bioactive coating seems to be an efficient strategy to improve soft tissue integration on zirconia implants, protecting implants from peri-implant inflammation and improving long-term implant stabilization. This new approach of micropatterned silica coating on zirconia substrates can generate promising novel dental implants, with surfaces that provide physical cues to guide cells and enhance their behavior.

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“…The micropatterned silica films were capable of inducing guided osteoblastic cell adhesion, spreading and propagation 56 . Isotropic and anisotropic surfaces changes cell-material and cell-cell interactions 57 .…”

Section: Discussionmentioning

confidence: 99%

Lithograph-moulded poly-L-co-D,L lactide porous membranes for osteoblastic culture

et al. 2013

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Pore size, shape, wall morphology, porosity, and interconnectivity are important characteristics of the scaffolds. Lithography is a manufacturing technique that allows the production of tridimensional scaffolds with a controllable and reproducible inner architecture. The aim of this study was to use lithography to create a poly-L-co-D,L lactide (PLDLA) scaffold with symmetrical pore size and distribution, and to evaluate its biocompatibility with osteoblasts in vitro. Lithographic moulds were used to produce porous PLDLA membranes by a casting procedure. Osteoblasts were removed from calvarial bones and seeded onto porous and smooth PLDLA membranes after which cell viability and adhesion assays, cytochemical analysis and scanning electron microscopy were used to characterize the cells. Cell viability and adhesion assays, cytochemical analysis, and scanning electron microscopy were carried out. Cell viability was similar on porous and smooth PLDLA membranes but higher than on a polystyrene substrate (positive control). Although osteoblasts adhered to the surface of all the materials tested, cell adhesion to lithographed PLDLA was greater than to smooth PLDLA membranes. In conclusion, osteoblasts interacted well with PLDLA membranes, as shown by the viability and adhesion assays and by the enhanced collagen production.

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Isotropic micropatterned silica coatings on zirconia induce guided cell growth for dental implants

Cited by 50 publications

References 69 publications

Relevant insight of surface characterization techniques to study covalent grafting of a biopolymer to titanium implant and its acidic resistance

Relevant insight of surface characterization techniques to study covalent grafting of a biopolymer to titanium implant and its acidic resistance

Modulation of human dermal microvascular endothelial cell and human gingival fibroblast behavior by micropatterned silica coating surfaces for zirconia dental implant applications

Lithograph-moulded poly-L-co-D,L lactide porous membranes for osteoblastic culture

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