Isotopic labelling-based analysis elucidates biosynthesis pathways in Saccharomyces cerevisiae for Melatonin, Serotonin and Hydroxytyrosol formation

Gallardo-Fernández, Marta; Valls-Fonayet, J.; Valero, Eva; Hornedo-Ortega, Ruth; Richard, Tom L.; Troncoso, Ana M.; García-Parrilla, M.C.

doi:10.1016/j.foodchem.2021.131742

Cited by 12 publications

(7 citation statements)

References 34 publications

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“…They ascertained the importance of the anabolic pathway in the formation of fusel alcohols, as more than 75% of the fusel alcohols associated with amino acid metabolism were hexose derived. Furthermore, HT synthesis by yeast has been elucidated by adding isotopically labeled tyrosine to the initial synthetic must [7]. Apart from the intermediates of the Ehrlich pathway ending in tyrosol, the presence of labeled and, most importantly, non-labeled HT was evidenced in this work.…”

Section: Introductionmentioning

confidence: 84%

“…Related to this, our results show increases in tyrosol but also in HT, which has the highest sugar content. Indeed, Gallardo-Fernández et al [7] found that HT was mainly formed from another source that was different from the initial tyrosine in the must using isotopically labeled precursors. Our results suggest that a high content of sugar could be a more relevant factor than YAN to HT production, as shown in Figure 6.…”

Section: Discussionmentioning

confidence: 99%

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The Production of Bioactive Hydroxytyrosol in Fermented Beverages: The Role of Must Composition and a Genetically Modified Yeast Strain

Gonzalez-Ramirez,

Gallardo-Fernandez,

Cerezo

et al. 2024

Fermentation

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Hydroxytyrosol (HT) is a well-known compound for its bioactive properties. It is naturally present in olives, olive oil, and wine. Its presence in wines is partly due to its production during alcoholic fermentation by yeast through a hydroxylation of tyrosol formed through the Ehrlich pathway. This work aims to explore the influence of yeast assimilable nitrogen (YAN) and glucose content as precursors of HT formation during alcoholic fermentation. Commercial Saccharomyces cerevisiae QA23 and its metabolically engineered strain were used to ferment synthetic must. Each strain was tested at two different YAN concentrations (210 and 300 mg L−1) and two glucose concentrations (100 and 240 g L−1). This work confirms that the less YAN and the more glucose, the higher the HT content, with fermentations carried out with the metabolically engineered strain being the ones with the highest HT content (0.6 mg L−1).

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Section: Introductionmentioning

confidence: 84%

Section: Discussionmentioning

confidence: 99%

The Production of Bioactive Hydroxytyrosol in Fermented Beverages: The Role of Must Composition and a Genetically Modified Yeast Strain

Gonzalez-Ramirez,

Gallardo-Fernandez,

Cerezo

et al. 2024

Fermentation

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“…Based on our data, SRT increased in T. rubrum transcripts of the gene encoding an aromatic-L-amino acid decarboxylase (EC 4.1.1.28) slightly below the log2 fold threshold (1.36 fold) ( Figure 5 ). This enzyme catalyzes the conversion of 5-hydroxytryptophan to serotonin [ 36 , 37 ]. In Aspergillus spp., serotonin can directly kill fungi in vitro [ 64 ].…”

Section: Discussionmentioning

confidence: 99%

“…In pink, transcript accumulation is slightly below the log2 fold threshold (corresponding to 1.36 fold). Figure adapted from [ 36 , 37 ].…”

Section: Figurementioning

confidence: 99%

The Antidepressant Sertraline Affects Cell Signaling and Metabolism in Trichophyton rubrum

Galvão-Rocha

Rocha

Martins

et al. 2023

JoF

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The dermatophyte Trichophyton rubrum is responsible for most human cutaneous infections. Its treatment is complex, mainly because there are only a few structural classes of fungal inhibitors. Therefore, new strategies addressing these problems are essential. The development of new drugs is time-consuming and expensive. The repositioning of drugs already used in medical practice has emerged as an alternative to discovering new drugs. The antidepressant sertraline (SRT) kills several important fungal pathogens. Accordingly, we investigated the inhibitory mechanism of SRT in T. rubrum to broaden the knowledge of its impact on eukaryotic microorganisms and to assess its potential for future use in dermatophytosis treatments. We performed next-generation sequencing (RNA-seq) to identify the genes responding to SRT at the transcript level. We identified that a major effect of SRT was to alter expression for genes involved in maintaining fungal cell wall and plasma membrane stability, including ergosterol biosynthetic genes. SRT also altered the expression of genes encoding enzymes related to fungal energy metabolism, cellular detoxification, and defense against oxidative stress. Our findings provide insights into a specific molecular network interaction that maintains metabolic stability and is perturbed by SRT, showing potential targets for its strategic use in dermatophytosis.

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“…Additionally, α-keto acids produced during amino acid biosynthesis from sugars can undergo degradation to form heteroalcohols. Gallardo-Fernandez et al [10] employed isotopic labeling analysis to investigate HT biosynthesis in Saccharomyces cerevisiae . The study revealed the presence of unlabeled compounds alongside tyrosine, suggesting the existence of an alternative pathway for HT synthesis.…”

Section: Natural Biosynthetic Pathway Of Hydroxytyrosolmentioning

confidence: 99%

Biosynthesis and Biotechnological Synthesis of Hydroxytyrosol

Tang,

Wang,

Gong

et al. 2023

Preprint

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Background： Hydroxytyrosol (HT), a phenolic compound derived from plants, exhibits robust antioxidant activity and possesses pharmacological properties, including anti-inflammatory, anti-cancer, anti-bacterial, and anti-viral effects. As a result, HT has gained significant attention as an excellent nutraceutical and food additive. Purpose and scope： Currently HT is mainly produced by plant extraction and chemical synthesis. With the gradual development of biosynthesis technology and the increasing concern for food health, biosynthesis of HT has gained more and more attention from researchers. Summary and conclusion: Biotechnological synthesis of HT offers distinct advantages over plant extraction and chemical synthesis, such as being environmentally friendly, safe, and cost-effective. This comprehensive review focuses specifically on the biosynthetic pathway of HT and recent advancements in biotechnological synthesis of HT. The review also discusses the potential applications of HT as a nutraceutical. Through a comprehensive analysis of the biosynthesis and biotechnological synthesis of HT, this review contributes to the growing body of knowledge in this field and paves the way for sustainable and scalable production of this versatile compound.

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Isotopic labelling-based analysis elucidates biosynthesis pathways in Saccharomyces cerevisiae for Melatonin, Serotonin and Hydroxytyrosol formation

Cited by 12 publications

References 34 publications

The Production of Bioactive Hydroxytyrosol in Fermented Beverages: The Role of Must Composition and a Genetically Modified Yeast Strain

The Production of Bioactive Hydroxytyrosol in Fermented Beverages: The Role of Must Composition and a Genetically Modified Yeast Strain

The Antidepressant Sertraline Affects Cell Signaling and Metabolism in Trichophyton rubrum

Biosynthesis and Biotechnological Synthesis of Hydroxytyrosol

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