Isotopic Analysis of Fe in Human Red Blood Cells by Multiple Collector-ICP-Mass Spectrometry

Ohno, Takeshi; Shinohara, Atsuko; Kohge, Ichitaro; Chiba, Momoko; Hirata, Takafumi

doi:10.2116/analsci.20.617

Cited by 61 publications

(68 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Stability of the iron isotopic signature in blood over one year was recently shown in a healthy subject. 20 In the present study, we show that patients with hemochromatosis have an altered iron isotopic composition of blood when compared with healthy controls. This strengthens the hypothesis that the iron isotopic composition of blood is primarily determined by the efficiency at which iron is absorbed from the diet.…”

Section: Introductionsupporting

confidence: 55%

“…This is not the case for iron isotope effects in the blood, which-if not perturbed-are stable during months or even years as recently shown. 20 …”

Section: Discussionmentioning

confidence: 99%

“…19 Each individual bears a distinct 56 Fe/ 54 Fe isotope ratio in the blood that is, on average, lower in males than in females. [19][20] It has been hypothesized that the iron isotopic composition is altered during intestinal absorption. Stability of the iron isotopic signature in blood over one year was recently shown in a healthy subject.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Hereditary hemochromatosis is reflected in the iron isotope composition of blood

Krayenbuehl

Walczyk

Schoenberg

et al. 2005

Blood

View full text Add to dashboard Cite

It has recently been shown that the iron isotopic composition of blood differs between individuals and sexes, which is supposed to reflect individual differences in iron metabolism. We hypothesized that patients suffering from hereditary hemochromatosis would demonstrate alterations in the iron isotopic composition of blood due to persistent up-regulation of intestinal iron absorption. Blood from 30 patients with homozygous C282Y hemochromatosis was analyzed for iron isotopic composition by a newly developed technique using multicollector inductively coupled plasma mass spectrometry (MC-ICP-MS). Blood of patients with hemochromatosis is characterized by a higher 56 Fe/ 54 Fe isotope ratio than blood of healthy individuals, which are either members of an age-matched control group (n ‫؍‬ 10; P < .001) or young adults (n ‫؍‬ 36; P < .001). In patients with hereditary hemochromatosis, the 56 Fe/ 54 Fe isotope ratio of blood significantly correlates with total-body iron accumulation, severity of clinical disease, and the need for regular phlebotomies to prevent iron reaccumulation. We conclude that blood of patients with hereditary hemochromatosis contains more of the heavier iron isotopes than blood of healthy individuals. The primary determinant of the iron isotopic composition of blood appears to be isotope-sensitive iron absorption in the intestine and the efficiency of this process. (Blood. 2005;105:3812-3816)

show abstract

Section: Introductionsupporting

confidence: 55%

“…This is not the case for iron isotope effects in the blood, which-if not perturbed-are stable during months or even years as recently shown. 20 …”

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Hereditary hemochromatosis is reflected in the iron isotope composition of blood

Krayenbuehl

Walczyk

Schoenberg

et al. 2005

Blood

View full text Add to dashboard Cite

show abstract

“…The level of isotopic fractionation can provide key information about the variation in dietary consumption or Ca metabolism. To investigate this, we measured the 43 Ca/ 42 Ca and 44 Ca/ 42 Ca ratios for bone and blood plasma samples collected from mice of various ages using multiple collector-ICP-mass spectrometry (MC-ICP-MS). The 44 Ca/ 42 Ca ratio in bones was significantly (0.44 -0.84‰) lower than the corresponding ratios in the diet, suggesting that Ca was isotopically fractionated during Ca metabolism for bone formation.…”

mentioning

confidence: 99%

Isotopic Analysis of Calcium in Blood Plasma and Bone from Mouse Samples by Multiple Collector-ICP-Mass Spectrometry

et al. 2008

Self Cite

View full text Add to dashboard Cite

The biological processing of Ca produces significant stable isotope fractionation. The level of isotopic fractionation can provide key information about the variation in dietary consumption or Ca metabolism. To investigate this, we measured the 43 Ca/ 42 Ca and 44 Ca/ 42 Ca ratios for bone and blood plasma samples collected from mice of various ages using multiple collector-ICP-mass spectrometry (MC-ICP-MS). The 44 Ca/ 42 Ca ratio in bones was significantly (0.44 -0.84‰) lower than the corresponding ratios in the diet, suggesting that Ca was isotopically fractionated during Ca metabolism for bone formation. The resulting 44 Ca/ 42 Ca ratios for blood plasma showed almost identical, or slightly higher, values (0.03 -0.2‰) than found in a corresponding diet. This indicates that a significant amount of Ca in the blood plasma was from dietary sources. Unlike that discovered for Fe, there were no significant differences in the measured 44 Ca/ 42 Ca ratios between female and male specimens (for either bone or blood plasma samples). Similarity, the 44 Ca/ 42 Ca ratios suggests that there were no significant differences in Ca dietary consumption or Ca metabolism between female and male specimens. In contrast, the 44 Ca/ 42Ca ratios of blood plasma from mother mice during the lactation period were significantly higher than those for all other adult specimens. This suggests that Ca supplied to infants through lactation was isotopically lighter, and the preferential supply of isotopically lighter Ca resulted in isotopically heavier Ca in blood plasma of mother mice during the lactation period. The data obtained here clearly demonstrate that the Ca isotopic ratio has a potential to become a new tool for evaluating changes in dietary consumption, or Ca metabolism of animals. both of these two isotope tracer approaches are based on the assumption that the spiked isotope replaces the native element, and behaves in an identical manner. 1This indicates that mimicing of the isotopes to the native nutrients must be considered in order to obtain reliable data for elemental metabolism. Faced with this problem, we have developed a new isotope tracer technique based on changes in the isotopic composition of Ca due to a mass-dependent isotopic effect. Hence, no spiking with additional elements, such as radio isotopes or enriched isotopes, was required, and therefore, no mimicing to the native nutrients is required. Moreover, it should be noted that the present stable isotope approach allows regular monitoring of the nutritional status of Ca of the donor without any spiking or addition of isotopes. The measurement of stable isotopes of nitrogen ( 15 N/ 14 N) and carbon ( 13 C/ 12 C) is a useful tool to trace trophic relationships and food webs. 3,12,13 This method assumes that, during the assimilation process, there is an enrichment in heavy isotopes (i.e., 13 C or 15 N) compared to the dietary intake, which is compensated by the preferential excretion of lighter isotopes. 14,15 The isotopic composition of an animals' tissue reflec...

show abstract

“…The samples were precipitated at pH ≈ 10 with 25 % NH 3 and solutions were equilibrated for 1 h before centrifugation. The supernate solutions were discarded; the precipitates were washed twice with Milli-Q H 2 O and then re-dissolved in HNO 3 . The Fe concentration was measured before and after the precipitation step with ICP-OES, to ensure a near-quantitative (±10 %) recovery during precipitation, which is essential to avoid Fe isotope fractionation.…”

Section: Methodsmentioning

confidence: 99%

The Iron Stable Isotope Fingerprint of the Human Diet

Blanckenburg

Noordmann

Guelke-Stelling

2013

J. Agric. Food Chem.

View full text Add to dashboard Cite

The stable isotopes of iron disclose the metabolic pathways of iron within the human food chain. We have measured with precise multicollector ICP-MS the iron concentrations and stable isotope composition of 60 food products that are representative of the average German diet. We find that vegetable falls within the range typical of strategy I plants (-0.1 ‰ to -1.4 ‰ in δ 56 Fe), crop products and processed crop food into the range typical of strategy II plants (-0.6 ‰ to +0.4 ‰), and animal products into the 54 Fe-enriched range known for animal tissue and blood (-1.1 ‰ to -2.7 ‰). Weighting these isotope compositions by the average iron dietary sources, we find a representative composition of European vegetarian diet of -0.45 ‰, while that of omnivores is -0.82 ‰. For human blood, known to be enriched in light iron isotopes, we find a fractionation factor for iron absorption of -2.0 ‰ to -2.3 ‰ for vegetarians (female and male, respectively), and -1.3 ‰ to -1.5 ‰ for omnivores (female and male, respectively). Knowing these fractionation factors is a prerequisite for using stable iron isotope ratios in blood as monitors of intestinal iron uptake regulation.

show abstract

Isotopic Analysis of Fe in Human Red Blood Cells by Multiple Collector-ICP-Mass Spectrometry

Cited by 61 publications

References 17 publications

Hereditary hemochromatosis is reflected in the iron isotope composition of blood

Hereditary hemochromatosis is reflected in the iron isotope composition of blood

Isotopic Analysis of Calcium in Blood Plasma and Bone from Mouse Samples by Multiple Collector-ICP-Mass Spectrometry

The Iron Stable Isotope Fingerprint of the Human Diet

Contact Info

Product

Resources

About