Isotope tag method for quantitative analysis of carbohydrates by liquid chromatography–mass spectrometry

Tags for the Stable Isotopic Labeling of Carbohydrates and Quantitative Analysis by Mass Spectrometry

“…[16][17][18] Experience with the ICAT reagent 21 has shown chromatographic resolution of the isotopically varied analytes 22 and fragmentation of the tag. 23 These problems have been overcome by the incorporation of 13 C in place of deuterium and an acid cleavable linker to the biotin which reduces the size and fragmentation complexity of the tag.…”

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“…Isotope labeling oligosaccharides in the mass spectrometric analyses is also quite useful for the identiˆcation of glycosylation sites (69)(70)(71) and quantitative analysis of oligosaccharide mixtures (72,73). It is, however, not feasible to determine the oligosaccharide structure unambiguously solely on the MS data because there exist a variety of isomeric structures with diŠerent composition, glycosidic linkage and branching.…”

Tandem Mass Spectrometry of Tagged and Permethylated Polysaccharides

“…A number of reductive amination reagents are commercially available (Yuan et al, 2005;Hitchcock et al, 2006;Zaia, 2008). During the derivatization, an aromatic amine forms a Schiff base at the acyclic reducing sugar residue.…”