Isotope equilibration measurements in perfused rat liver synthesizing glucose from L‐lactate‐2‐T‐2‐C<sup>14</sup>

Müllhofer, Gerd; Kuntzen, O.; Hesse, S.; Bücher, Th.

doi:10.1016/0014-5793(69)80192-4

Cited by 11 publications

(2 citation statements)

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“…When [2-3 H]lactate is used as a tracer, sequestration and formation of labeled products occur in a different manner. Lactate dehydrogenase will mediate the transfer of 3 H to the ␣-position of NADH, from where it can be transferred to other metabolic intermediates by the action of other NADH-dependent dehydrogenases (24,30). This transfer is expected to be slower than that of the 14 C label, because it requires the dissociation of the coenzyme from the enzyme.…”

Section: G780mentioning

confidence: 99%

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Uptake of lactate by the liver: effect of red blood cell carriage

Goresky

Bach

Simard

et al. 2000

American Journal of Physiology-Gastrointestinal and Liver Physi

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Multiple-indicator dilution experiments with labeled lactate were performed in the livers of anesthetized dogs. A mixture of (51)Cr-labeled erythrocytes, [(3)H]sucrose, and L-[1-(14)C]lactate or a mixture of (51)Cr-labeled erythrocytes, [(14)C]sucrose, and L-[2-(3)H]lactate was injected into the portal vein, and samples were obtained from the hepatic vein. Data were evaluated using a model comprising flow along sinusoids, exchange of lactate between plasma and erythrocytes and between plasma and hepatocytes, and, in the case of L-[1-(14)C]lactate, metabolism to H[(14)C]O(-)(3) within hepatocytes. The coefficient for lactate efflux from erythrocytes was 0.62 +/- 0.24 s(-1), and those for influx into and efflux from hepatocytes were 0.44 +/- 0.13 and 0.14 +/- 0.07 s(-1), respectively. The influx permeability-surface area product of the hepatocyte membrane for lactate (P(in)S, in ml x s(-1) x g(-1)) varied with total flow rate (F, in ml s(-1) x g(-1)) according to P(in)S = (3.1 +/- 0.5)F + (0.021 +/- 0.014). Lactate in plasma, erythrocytes, and hepatocytes was close to equilibrium, whereas lactate metabolism was rate limiting.

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Section: G780mentioning

confidence: 99%

“…Part of the label will eventually appear as 3 HOH because of oxidation reactions and hydrogen exchange with cellular water. Another part may be incorporated into metabolites released by the liver, such as into glucose formed by gluconeogenesis (30,52). A detailed analysis of the fate of the 3 H label is beyond the scope of this work.…”

Section: G780mentioning

confidence: 99%

Uptake of lactate by the liver: effect of red blood cell carriage

Goresky

Bach

Simard

et al. 2000

American Journal of Physiology-Gastrointestinal and Liver Physi

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The State of the DPN System in Liver. An Analysis of Pyridine Nucleotide Levels, Surface Fluorescence, and Redox Potentials of Indicator Metabolite Couples in the Hemoglobin-Free Perfused Rat Liver

Bücher

1970

Pyridine Nucleotide-Dependent Dehydrogenases

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Pathways of reducing equivalents in hepatocytes from starved, ethanol-induced, and hyperthyroid rats during ethanol and xylitol metabolism

Vind

Grunnet

1981

Archives of Biochemistry and Biophysics

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Isotope equilibration measurements in perfused rat liver synthesizing glucose from L‐lactate‐2‐T‐2‐C¹⁴

Cited by 11 publications

References 9 publications

Uptake of lactate by the liver: effect of red blood cell carriage

Uptake of lactate by the liver: effect of red blood cell carriage

The State of the DPN System in Liver. An Analysis of Pyridine Nucleotide Levels, Surface Fluorescence, and Redox Potentials of Indicator Metabolite Couples in the Hemoglobin-Free Perfused Rat Liver

Pathways of reducing equivalents in hepatocytes from starved, ethanol-induced, and hyperthyroid rats during ethanol and xylitol metabolism

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Isotope equilibration measurements in perfused rat liver synthesizing glucose from L‐lactate‐2‐T‐2‐C14

Cited by 11 publications

References 9 publications

Uptake of lactate by the liver: effect of red blood cell carriage

Uptake of lactate by the liver: effect of red blood cell carriage

The State of the DPN System in Liver. An Analysis of Pyridine Nucleotide Levels, Surface Fluorescence, and Redox Potentials of Indicator Metabolite Couples in the Hemoglobin-Free Perfused Rat Liver

Pathways of reducing equivalents in hepatocytes from starved, ethanol-induced, and hyperthyroid rats during ethanol and xylitol metabolism

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Isotope equilibration measurements in perfused rat liver synthesizing glucose from L‐lactate‐2‐T‐2‐C¹⁴