Isotope dilution gas chromatographic-mass spectrometric method for the determination of lignans and isoflavonoids in human urine, including identification of genistein

Adlercreutz, Herman; Fotsis, Theodore; Bannwart, C.; Wähälä, Kristiina; Brunow, Gösta; Hase, Tapio

doi:10.1016/0009-8981(91)90120-2

Cited by 180 publications

(105 citation statements)

References 34 publications

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“…Samples were analyzed for phytoestrogens, including the 3 major soy isoflavones (daidzein, genistein, glycitein) and 2 metabolites of daidzein [O-desmethylangolensin (ODMA) and equol], using an ion-exchange chromatography and capillary gas chromatography-mass spectrometry method (21) in which phytoestrogens were first extracted on Bond Elut C 18 columns. Deuterated phytoestrogen and estrogen internal standards were added to each sample.…”

Section: Assessment Of Urinary Phytoestrogensmentioning

confidence: 99%

Food Frequency Questionnaires and Overnight Urines Are Valid Indicators of Daidzein and Genistein Intake in U.S. Women Relative to Multiple 24-h Urine Samples

Tseng

Olufade

Kurzer

et al. 2008

Nutrition and Cancer

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Data regarding convenient, valid methods for measuring U.S. isoflavone intake are limited. We evaluated a soy food questionnaire (SFQ), the Willett food frequency questionnaire (FFQ), and overnight urine samples relative to excretion in 24-h urine samples. We also described intake among women in a high-risk program for breast or ovarian cancer. Between April 2002 and June 2003, 451 women aged 30 to 50 yr with a family history of breast or ovarian cancer completed the SFQ and FFQ. Of them, 27 provided four 24-h and overnight urine specimens. In these women, 24-h sample measures were correlated with SFQ estimates of daidzein (Spearman r = .48) and genistein (r = .54) intake, moderately correlated with the Willett FFQ (daidzein r = .38, genistein r = .33), and strongly correlated with overnight urine excretion (daidzein r = .84, genistein r = 0.93). Among all 451 SFQ respondents, mean (median) daidzein and genistein intakes were 2.8 (0.24) and 3.9 (0.30) mg/day. Primary sources of both were soymilk, soy nuts, and tofu. We conclude that targeted soy food questionnaires, comprehensive FFQs, and multiple overnight urines are all reasonable options for assessing isoflavone intake in epidemiologic studies.

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Section: Assessment Of Urinary Phytoestrogensmentioning

confidence: 99%

Food Frequency Questionnaires and Overnight Urines Are Valid Indicators of Daidzein and Genistein Intake in U.S. Women Relative to Multiple 24-h Urine Samples

Tseng

Olufade

Kurzer

et al. 2008

Nutrition and Cancer

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“…Of the isoflavonoids, we can now determine daidzein, 0-demethylangolensin, equol, and genistein in plasma (27), urine (28), and feces (29) allowing studies on absorption and metabolism of these compounds.…”

Section: Metabolism Of Phytoestrogens In Manmentioning

confidence: 99%

Phytoestrogens: Epidemiology and a Possible Role in Cancer Protection

Adlercreutz¹

1995

Environmental Health Perspectives

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Because many diseases of the Western Hemisphere are hormone-dependent cancers, we have postulated that the Western diet, compared to a vegetarian or semivegetarian diet, may alter hormone production, metabolism, or action at the cellular level by some biochemical mechanisms. Recently, our interest has been mainly focused on the cancer-protective role of some hormonelike diphenolic phytoestrogens of dietary origin, the lignans and the isoflavonoids. The precursors of the biologically active compounds originate in soybean products (mainly isoflavonoids), whole grain cereal food, seeds, and probably berries and nuts (mainly lignans). The plant lignan and isoflavonoid glycosides are converted by intestinal bacteria to hormonelike compounds with weak estrogenic but also antioxidative activity; they have now been shown to influence not only sex hormone metabolism and biological activity but also intracellular enzymes, protein synthesis, growth factor action, malignant cell proliferation, differentiation, and angiogenesis in a way that makes them strong candidates for a role as natural cancer-protective compounds. Epidemiologic investigations strongly support this hypothesis because the highest levels of these compounds in the diet are found in countries or regions with low cancer incidence. This report is a review on recent results suggesting that the diphenolic isoflavonoids and lignans are natural cancer-protective compounds. -Environ Health Perspect 103(Suppl 7):103-1 12 (1995)

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“…They are enterolactone (Enl). enterodiol (End), matairesinol (Mat), daidzein (Da), equol (Eq, O-desmethylangolensin ( 0-Dma), and genistein (Gen) (structures and systematic names in 26,29). It was shown that En1 and a theoretical intermediate between Mat and En1 are moderate inhibitors of placental aromatase ( I , 8).…”

Section: Lignans and Isoflavonoid Phytoestrogensmentioning

confidence: 99%

“…Of about 15 compounds identified ( I , 8, 26) 7 can now be measured by isotope dilution mass spectrometry (24,29). They are enterolactone (Enl).…”

Section: Lignans and Isoflavonoid Phytoestrogensmentioning

confidence: 99%

“…These diphenols are readily transferred from cell culture media into the cells and may inhibit cancer cell growth, since antiproliferative effects of some naturally occurring flavonoids (30) and of the lignans En1 and End and other lignans (28,31,32) with regard to certain estrogen sensitive BC cell lines have been reported. Genistein, recently identified and quantified by us in human urine (29,33), is a specific inhibitor of several tyrosine-specific protein kinases (34)(35)(36) with the exception of the p40 proteintyrosine kinase (37). It also inhibits the interleukin-I stimulated production of prostaglandin E2 in mesangial cells (38).…”

Section: Lignans and Isoflavonoid Phytoestrogensmentioning

confidence: 99%

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