Isotachophoretic separation of polyols in boric acid solutions

A method for isotachophoretic determination of sweeteners of different character in candies and chewing gums was developed. A capillary of 0.8 mm ID and 90 mm effective length made of fluorinated ethylene-propylene copolymer is filled with an electrolyte system consisting of 10 mM HCl + 14 mM Tris, pH 7.7 (leading electrolyte) and 5 mM L-histidine + 5 mM Tris, pH 8.3 (terminating electrolyte). The analysis is performed at a driving current of 200 microA and for detection current is decreased to 100 microA. Boric acid is added to the aqueous sample solution to form borate complexes with substances of polyhydroxyl nature and make them migrate isotachophoretically. Using conductivity detection, the calibration curves in the tested concentration range up to 2.5 mM were linear for all components of interest: acesulfame K, saccharine, aspartame, cyclamate, sorbitol, mannitol, lactitol, and xylitol. The concentration detection limits ranged between 0.024 and 0.081 mM. Good precision of the ITP method is evidenced by favorable RSD values ranging from 0.8 to 2.8% obtained at the analyte concentration of 1.0 mM (n = 6). The analysis time was about 20 min. Simplicity, accuracy, and low cost of analyses make ITP an alternative procedure to methods used so far for the determination of ionizable sweeteners.

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“…Compared with free boric acid, the complexes with diols are stronger acids and possess higher electrophoretic mobility [30,31]. [27].…”

Section: Mobility Calculationsmentioning

confidence: 99%

Direct simultaneous determination of eight sweeteners in foods by capillary isotachophoresis

Herrmannová

Křivánková²,

Bartoš

et al. 2006

J of Separation Science

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On-capillary raman spectroscopy of nucleoside/borate complexes separated by isotachophoresis

Walker

Morris

1998

J. Micro. Sep.

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On-capillary normal Raman spectroscopy with 532 nm excitation is Ž. used as a detection method for capillary isotachophoresis ITP of the nucleosides: adenosine, cytidine, guanosine, and uridine. The compounds are separated as their borate complexes between leading electrolyte 0.1 M Cl y , pH 7.5, and terminating Ž. y electrolyte 0.1 M B OH , pH 10. The Raman spectra of the nucleosiderborate 4 complexes are presented. Additionally, Raman spectra are obtained from sepa-X Ž. rated adenosine and adenosine 5-triphosphate ATP. Starting concentrations in the range 5.0 = 10 y6 ᎐5 = 10 y4 M are employed. Preconcentration factors of at least 10 3 are observed.

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Isotachophoresis of polyols in borate buffer solutions

Reijenga

1994

Journal of Chromatography A

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Isotachophoretic separation of polyols in boric acid solutions

Cited by 6 publications

References 3 publications

Direct simultaneous determination of eight sweeteners in foods by capillary isotachophoresis

Direct simultaneous determination of eight sweeteners in foods by capillary isotachophoresis

On-capillary raman spectroscopy of nucleoside/borate complexes separated by isotachophoresis

Isotachophoresis of polyols in borate buffer solutions

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