Direct simultaneous determination of eight sweeteners in foods by capillary isotachophoresis

Abstract

A method for isotachophoretic determination of sweeteners of different character in candies and chewing gums was developed. A capillary of 0.8 mm ID and 90 mm effective length made of fluorinated ethylene-propylene copolymer is filled with an electrolyte system consisting of 10 mM HCl + 14 mM Tris, pH 7.7 (leading electrolyte) and 5 mM L-histidine + 5 mM Tris, pH 8.3 (terminating electrolyte). The analysis is performed at a driving current of 200 microA and for detection current is decreased to 100 microA. Boric acid is added to the aqueous sample solution to form borate complexes with substances of polyhydroxyl nature and make them migrate isotachophoretically. Using conductivity detection, the calibration curves in the tested concentration range up to 2.5 mM were linear for all components of interest: acesulfame K, saccharine, aspartame, cyclamate, sorbitol, mannitol, lactitol, and xylitol. The concentration detection limits ranged between 0.024 and 0.081 mM. Good precision of the ITP method is evidenced by favorable RSD values ranging from 0.8 to 2.8% obtained at the analyte concentration of 1.0 mM (n = 6). The analysis time was about 20 min. Simplicity, accuracy, and low cost of analyses make ITP an alternative procedure to methods used so far for the determination of ionizable sweeteners.

Simultaneous determination of aspartame, cyclamate, saccharin and acesulfame-K in soft drinks and tabletop sweetener formulations by capillary electrophoresis with capacitively coupled contactless conductivity detection

“…Good linearity was obtained for all sweeteners over large ranges of concentrations. Limits of detection (LOD) and quantification (LOQ) were comparable with those obtained by the cited isotachophoresis method (Herrmannová et al, 2006). These analytical parameters were also considered in the most cases better than ones for CE methods with photometric detection (Frazier et al, 2000;Horie et al, 2007;Huang, Chuang, Chiu, & Yeh, 2005;Walker, Zaugg, & Walker, 1997).…”

“…We can note that separations with good resolution can be obtained in less than 6 min. This separation time is smaller than half of that required in the previous isotachophoresis method (Herrmannová et al, 2006) also using conductivity detection. Although a positive separation voltage is applied in the CE-C 4 D method the anionic sweeteners are moved towards the cathode by the electroosmotic flow (EOF).…”

“…Conductivity detection is a good alternative method for compounds lacking a strong UV-absorbing moiety. Using this detection technique an isotachophoresis method was published for determination of sweeteners in chewing gums and candies (Herrmannová, Krivánková, Bartos, & Vytras, 2006). Capacitively coupled contactless conductivity detection (C 4 D) is a relatively new approach for detection on CE (CE-C 4 D) (Fracassi da Silva & do Lago, 1998;Fracassi da Silva, Guzman, & do Lago, 2002;Zemann, Schnell, Volgger, & Bonn, 1998 (Munoz, Richter, de Jesus, do Lago, & Angnes, 2004), biological (Fracassi da Silva, Ricelli, Carvalho, & do Lago, 2003), environmental (Rocha, Fracassi da Silva, do Lago, Fornaro, & Gutz, 2003;Rocha et al, 2008) and food (Escarpa et al, 2008;Richter, de Jesus, Munoz, do Lago, & Angnes, 2005) samples.…”

Direct Determination of Saccharin and Acesulfame-K in Sweeteners and Fruit Juices Powders

“…Furthermore, there are some papers reporting determination of sweeteners without chromatographic separation, most of them dealt with the determination of cyclamate join with another sweetener (Armenta et al 2004;Herrmannová et al 2006;Rocha et al 2005).…”

Quantification of aspartame in commercial sweeteners by FT-Raman spectroscopy

“…However, there are also examples of the use of capillary electrophoresis (Herrmannovà, Křivànkova, Bartoš, & Vytřas, 2005;Pesek & Matyska, 1997), flow injection (Capitán-Vallvey, Valencia, Nicolás, & García-Jiménez, 2006;Peña, Limba, & Saraiva, 2004), biosensors (Campanella, Aturki, Sammartino, & Tomassetti, 1995) and voltammetry (Medeiros, De Carvalho, Rocha-Filho, & Fatibello-Filho, 2008) applications for aspartame quantitative determination. Spectrophotometry (Cantarelli, Pellerano, Marchevsky, & Camiña, 2009) and infrared spectroscopy (Khurana, Cho, Shim, Li, & Jun, 2008;Kizil, Irudayaraj, & Seetharaman, 2002) in conjunction with multivariate techniques of data treatment were applied for aspartame quantification.…”