Quantification of aspartame in commercial sweeteners by FT-Raman spectroscopy

Mazurek, Sylwester; Szostak, Roman

doi:10.1016/j.foodchem.2010.09.075

Cited by 38 publications

(8 citation statements)

References 32 publications

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“…LC-MS methods are rapid, do not require extensive clean-up and offer low limits of detection with high specificity, but have, up to now, not been validated by any inter-laboratory studies. Other successful approaches for direct analysis of aspartame in commercial sweeteners have employed Fourier transform infrared spectroscopy (FT-IR) (Mazurek and Szostak, 2011), in soft drinks and tabletop sweeteners capillary electrophoresis with capacitively coupled contactless conductivity detection (CE-C 4 D) (Bergamo et al, 2011) and in carbonated beverages proton nuclear magnetic resonance ( 1 H-NMR) (Maes et al, 2012).…”

Section: Methods Of Analysis In Foodmentioning

confidence: 99%

Scientific Opinion on the re‐evaluation of aspartame (E 951) as a food additive

2013

EFS2

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The EFSA ANS Panel provides a scientific opinion on the safety of aspartame (E 951). Aspartame is a sweetener authorised as a food additive in the EU. In previous evaluations by JECFA and the SCF, an ADI of 40 mg/kg bw/day was established based on chronic toxicity in animals. Original reports, previous evaluations, additional literature and data made available following a public call were evaluated. Aspartame is rapidly and completely hydrolysed in the gastrointestinal tract to phenylalanine, aspartic acid and methanol. Chronic and developmental toxicities were relevant endpoints in the animal database. From chronic toxicity studies in animals, a NOAEL of 4000 mg/kg bw/day was identified. The possibility of developmental toxicity occurring at lower doses than 4000 mg/kg in animals could not be excluded. Based on MoA and weight‐of‐evidence analysis, the Panel concluded that developmental toxicity in animals was attributable to phenylalanine. Phenylalanine at high plasma levels is known to cause developmental toxicity in humans. The Panel concluded that human data on developmental toxicity were more appropriate for the risk assessment. Concentration‐response modelling was used to determine the effects of aspartame administration on plasma phenylalanine using human data after phenylalanine administration to normal, PKU heterozygote or PKU homozygote individuals. In normal and PKU heterozygotes, aspartame intakes up to the ADI of 40 mg/kg bw/day, in addition to dietary phenylalanine, would not lead to peak plasma phenylalanine concentrations above the current clinical guideline for the prevention of adverse effects in fetuses. The Panel concluded that aspartame was not of safety concern at the current aspartame exposure estimates or at the ADI of 40 mg/kg bw/day. Therefore, there was no reason to revise the ADI of aspartame. Current exposures to aspartame ‐ and its degradation product DKP ‐ were below their respective ADIs. The ADI is not applicable to PKU patients.

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Section: Methods Of Analysis In Foodmentioning

confidence: 99%

Scientific Opinion on the re‐evaluation of aspartame (E 951) as a food additive

2013

EFS2

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“…Vibrational spectroscopy as a non-destructive technique, including FTIR and FT-Raman, proposes a direct qualitative and quantitative determination of non-nutritive sweeteners in solid samples. The analytical methods based on these techniques usually do not need sample preparation steps [ 65 , 66 ].…”

Section: Conventional Methods For Non-nutritive Sweeteners Determinationmentioning

confidence: 99%

Analytical Methods for Determination of Non-Nutritive Sweeteners in Foodstuffs

et al. 2021

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Sweeteners have been used in food for centuries to increase both taste and appearance. However, the consumption of sweeteners, mainly sugars, has an adverse effect on human health when consumed in excessive doses for a certain period, including alteration in gut microbiota, obesity, and diabetes. Therefore, the application of non-nutritive sweeteners in foodstuffs has risen dramatically in the last decade to substitute sugars. These sweeteners are commonly recognized as high-intensity sweeteners because, in a lower amount, they could achieve the same sweetness of sugar. Regulatory authorities and supervisory agencies around the globe have established the maximum amount of these high-intensity sweeteners used in food products. While the regulation is getting tighter on the market to ensure food safety, reliable analytical methods are required to assist the surveillance in monitoring the use of high-intensity sweeteners. Hence, it is also necessary to comprehend the most appropriate method for rapid and effective analyses applied for quality control in food industries, surveillance and monitoring on the market, etc. Apart from various analytical methods discussed here, extraction techniques, as an essential step of sample preparation, are also highlighted. The proper procedure, efficiency, and the use of solvents are discussed in this review to assist in selecting a suitable extraction method for a food matrix. Single- and multianalyte analyses of sweeteners are also described, employing various regular techniques, such as HPLC, and advanced techniques. Furthermore, to support on-site surveillance of sweeteners’ usage in food products on the market, non-destructive analytical methods that provide practical, fast, and relatively low-cost analysis are widely implemented.

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“…The Fourier Transform (FT) technique can detect all wavelengths at the same time improving the resolution, acquisition time spectrum, and signal-to-noise (S/R) ratio of conventional Raman spectroscopy. All this combined with chemometric tools, i.e., partial least squares (PLS), principal component regression (PCR), and counter-propagation artificial neural networks (CP-ANN) methods, offer the possibility of reliable quantification of ASP in commercial sweeteners [105]. ASP concentrations in the range of 17.46-35.93 % were obtained in excipient formulations which contained, by weight, 55.8-76.9 % lactose, 2.7-5.5 % leucine, 2.9-4.7 % CMCNa, and up to 0.6 % magnesium stearate.…”

Section: Other Determination Techniquesmentioning

confidence: 99%

Analytical Strategies to Determine Artificial Sweeteners by Liquid Chromatography-Mass Spectrometry

Ferreira¹,

Yusty²,

Díaz³

2016

Reference Series in Phytochemistry

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The interest of consumers and government organizations on improving the quality of food and its impact on human health is growing in recent years. Consuming low-calorie foodstuffs are increasingly demanded, and the presence of artificial sweeteners plays an important role not only in food but also in waste reaching the environment. Analysis of these compounds is important in assessing food safety and quality. This chapter reviews the analytical approaches for the extraction and reliable identification and quantification of most commonly used artificial sweeteners in food, pharmaceutical, and environmental related matrices. The advantages and disadvantages of determination techniques used are described, with special emphasis on liquid chromatography coupled to mass

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Quantification of aspartame in commercial sweeteners by FT-Raman spectroscopy

Cited by 38 publications

References 32 publications

Scientific Opinion on the re‐evaluation of aspartame (E 951) as a food additive

Scientific Opinion on the re‐evaluation of aspartame (E 951) as a food additive

Analytical Methods for Determination of Non-Nutritive Sweeteners in Foodstuffs

Analytical Strategies to Determine Artificial Sweeteners by Liquid Chromatography-Mass Spectrometry

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