Isosinensetin alleviates the injury of human bronchial epithelial cells induced by PM_2.5

Abstract

Flavonoids which are extracted from citrus peel and pulp have been reported to have multiple beneficial effects on human health. Isosinensetin (ISO) is a type of flavonoid compound, which has several protective effects including anticancer, antioxidant, antiviral, anti-inflammatory and bacteriostatic. However, the molecular mechanism of its antioxidant and anti-inflammatory effects remain unclear. The present study aimed to investigate the intervention effect and possible mechanism of ISO on human bronchial epithelial cells injured by fine particular matter ≤2.5 µm in diameter (PM 2.5 ). In the present study, the cell viability was detected by Cell Counting Kit-8 method. The levels of pro-inflammatory cytokines were analyzed by ELISA. The level of reactive oxygen species (ROS) was detected by fluorescence probe. The expression levels of proliferating cell nuclear antigen (PCNA), nuclear factor erythroid 2-related factor 2 (Nrf2) and nuclear factor кΒ (NF-кB) proteins were detected by western blotting. The results revealed that ISO evidently increased the viability of 16-HBE cells and sharply decreased the levels of pro-inflammatory factors in cell culture supernatant. ISO significantly inhibited ROS release caused by PM 2.5 . Moreover, the expression levels of PCNA, Nrf2 and NF-кB proteins were downregulated after ISO incubation. These results indicated that ISO alleviated 16-HBE-cell injury by PM 2.5 through the ROS-Nrf2/NF-кB signaling pathway.

Synergistic effects of flavonoids and paclitaxel in cancer treatment: a systematic review

“…As a polymethoxylated flavone structure, isosinensetin can be found in a wide variety of plant species, including orange, and has been linked to a variety of health benefits [ 100 ]. P-glycoprotein in MDR1-MDCKII cells was significantly inhibited by isosinensetin, resulting in a reduction in the P-glycoprotein-mediated efflux of taxol and an increase in cellular toxicity.…”