Isoquercitrin Suppresses Colon Cancer Cell Growth in Vitro by Targeting the Wnt/β-Catenin Signaling Pathway

Amado, Nathália G.; Predes, Danilo; Fonseca, Bárbara F.; Cerqueira, Débora M.; Reis, Alice H.; Dudenhoeffer, Ana C.; Borges, Helena L.; Mendes, Fábio A.; Abreu, José G.

doi:10.1074/jbc.m114.621599

Cited by 87 publications

(64 citation statements)

References 39 publications

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“…Mutations and increases in nuclear level and activity of β‐catenin have been reported in malignant cells. Previous studies have demonstrated small molecule‐mediated and targeted inhibition of β‐catenin [Park and Choi, ; Amado et al, ; Gang et al, ]. Our studies demonstrate that 2‐ME treatment in osteosarcoma cells results in an increase in the cytoplasmic levels and a decrease in nuclear levels of β‐catenin.…”

Section: Discussionsupporting

confidence: 69%

2‐Methoxyestradiol‐Mediated Induction of Frzb Contributes to Cell Death and Autophagy in MG63 Osteosarcoma Cells

Bravo

Shogren

Zuo

et al. 2017

J of Cellular Biochemistry

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Osteosarcoma is a bone tumor that mainly affects children and adolescents. Although its pathogenesis is still not fully understood, activation of Wnt signaling has been implicated in the development and metastasis of osteosarcoma. In this report, we have investigated the effect of the anti-tumor compound, 2-methoxyestradiol (2-ME) on Wnt antagonist frizzled-related protein b (Frzb), also known as secreted frizzled-related protein (sFRP)3 in human osteosarcoma (MG63) cells. Our results show that 2-ME treatment induces Frzb gene promoter activity, and increases Frzb mRNA and protein levels in osteosarcoma cells. In addition, 2-ME treatment regulates downstream Wnt signaling, increasing the cytoplasmic levels of β-catenin, and blocking β-catenin-mediated Wnt activation in osteosarcoma cells. 2-ME-mediated induction of Frzb protein expression is specific to osteosarcoma cells, as it does not affect Frzb expression in normal primary human osteoblasts. Furthermore, 2-ME-induced apoptosis and autophagy are blocked in osteosarcoma cells transfected with Frzb siRNAs. Taken together, these studies demonstrate that Frzb protein plays an important role in 2-ME-mediated anti-tumor mechanisms in osteosarcoma cells. J. Cell. Biochem. 118: 1497-1504, 2017. © 2016 Wiley Periodicals, Inc.

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Section: Discussionsupporting

confidence: 69%

2‐Methoxyestradiol‐Mediated Induction of Frzb Contributes to Cell Death and Autophagy in MG63 Osteosarcoma Cells

Bravo

Shogren

Zuo

et al. 2017

J of Cellular Biochemistry

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“…IQ affects Xenopus axis establishment, reverses the double axes and LiCl hyperdorsalization phenotype, and reduces Xnr3 expression. IQ showed antitumoral effects on colon cancer cells (SW480, DLD1, and HCT116) whereas it exerted no significant effect on nontumor colon cells (IEC18), suggesting a specific effect on tumor cells in vitro [82] .…”

Section: Colon Cancermentioning

confidence: 99%

Review of anticancer mechanisms of isoquercitin

Orfali¹,

Duarte²,

Bonadio³

et al. 2016

WJCO

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This review was based on a literature search of PubMed and Scielo databases using the keywords "quercetin, rutin, isoquercitrin, isoquercitin (IQ), quercetin-3-glucoside, bioavailability, flavonols and favonoids, and cancer" and combinations of all the words. We collected relevant scientific publications from 1990 to 2015 about the absorption, bioavailability, chemoprevention activity, and treatment effects as well as the underlying anticancer mechanisms of isoquercitin. Flavonoids are a group of polyphenolic compounds widely distributed throughout the plant kingdom. The subclass of flavonols receives special attention owing to their health benefits. The main components of this class are quercetin, rutin, and IQ, which is a flavonoid and although mostly found as a glycoside, is an aglycone (lacks a glycoside side chain). This compound presents similar therapeutic profiles to quercetin but with superior bioavailability, resulting in increased efficacy compared to the aglycone form. IQ has therapeutic applications owing to its wide range of pharmacological effects including antioxidant, antiproliferative, anti-inflammatory, anti-hypertensive, and anti-diabetic. The protective effects of IQ in cancer may be due to actions on lipid peroxidation. In addition, the antitumor effect of IQ and its underlying mechanism are related to interactions with Wnt signaling pathway, mixed-lineage protein kinase 3, mitogen-activated protein kinase, apoptotic pathways, as well proinflammatory protein signaling. This review contributed to clarifying the mechanisms of absorption, metabolism, and actions of IQ and isoquercitrin in cancer.

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“…Moreover, the growing epidemiologic evidence has demonstrated that flavonoid consumption is associated with a decreased risk of colorectal cancer, which has recently been documented by a large casecontrol study [7]. Hitherto, a number of flavonoids, exemplified by isoquercitrin [8], quercetin [9], and catechin [10] have been shown to exert anti-colorectal cancer activity by inhibiting cell proliferation.…”

Section: Introductionmentioning

confidence: 99%

Antioxidant and Anti-proliferative Activities of Flavonoids from <i>Bidens pilosa</i> L var <i>radiata</i> Sch Bip

Yi¹,

Wu²,

Wu³

et al. 2016

Trop. J. Pharm Res

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Isoquercitrin Suppresses Colon Cancer Cell Growth in Vitro by Targeting the Wnt/β-Catenin Signaling Pathway

Cited by 87 publications

References 39 publications

2‐Methoxyestradiol‐Mediated Induction of Frzb Contributes to Cell Death and Autophagy in MG63 Osteosarcoma Cells

2‐Methoxyestradiol‐Mediated Induction of Frzb Contributes to Cell Death and Autophagy in MG63 Osteosarcoma Cells

Review of anticancer mechanisms of isoquercitin

Antioxidant and Anti-proliferative Activities of Flavonoids from <i>Bidens pilosa</i> L var <i>radiata</i> Sch Bip

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