Isoquercetin Could Protect Against Ovariectomy-Induced Neuronal Changes in Rats

Abstract

Menopause occurs gradually and is characterized by increased susceptibility to developing mood disorders. Several studies have suggested that oxidative stress is implicated in the subsequent mood changes. It has been reported that quercetin glycosides may be effective due to their antioxidant abilities. The present work aimed to find out whether quercetin able to act against ovariectomy consequences and possible underlying mechanism(s). Animals were randomly divided into six groups of eight rats each. Group (1) Control group (2) Sham operated group (3) Ovariectomized (OVX) group (4) OVX-Isoquercetin-treated (10 mg/kg, i.p., dissolved in a DMSO/saline solution) group (5) OVX-estrogen-treated (subcutaneous implant of pellets (Innovative Research of America, Toledo, OH) containing 17β-estradiol (1.5 mg/8 wk) group (6) OVX-Isoquercetin-estrogen treated group. The treatments were initiated two week after both ovariectomy and sham operations and continued for four consecutive weeks. Tested parameters: oxidative stress markers (MDA, GSH, SOD), inflammatory cytokines (TNF-α, IL-6) and brain monoamines (dopamine, nor epinephrine, 5-HT). Results: Isoquercetin either alone or in combination with estrogen can improve: oxidative stress markers (MDA, GSH, SOD), inflammatory cytokines (TNF-α, IL-6) and brain monoamines (dopamine, nor epinephrine, 5-HT). Combination of both isoquercetin & estrogen gives the best results in most of the tested parameters especially in normalizing IL-6 level. Concerning serotonin estrogen was as good as the combined drugs. Conclusion: Isoquercetin has an additive role with estrogen to maintain healthy brain tissue for production of normal monoamine levels.

Antidepressant Potential of Quercetin and its Glycoside Derivatives: A Comprehensive Review and Update

“…For example, the depression-like behaviors of mice were alleviated after treatment with isoquercetin for 2 weeks, with the reduction of ACTH and CORT levels (Butterweck et al, 2004). Another study showed that isoquercetin, either in monotherapy or in combination with estrogen, could significantly improve oxidative stress, proinflammatory cytokines, and brain monoamine concentration in ovariectomized rats (Elnoury, 2019). Martínez-Hernández et al (2021) found similar results.…”

Anti-Inflammatory Effect of Pterospartum tridentatum Leaf Extract in Acute and Chronic Inflammation

“…As previously shown, isoquercetin and genistein, phenolic compounds present in P. tridentatum extracts, could be linked to the scavenging of reactive oxygen species [38][39][40]. Nevertheless, our results in molecular docking analysis showed isoquercetin can also act as…”