2003
DOI: 10.1016/j.biochi.2003.09.001
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Isoproterenol increases active lipoprotein lipase in adipocyte medium and in rat plasma

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Cited by 48 publications
(30 citation statements)
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“…b3-AR are more stimulated by local NE and less by E than for example b2-AR and this is due to the reduced affinity to E of b3-AR compared to b1-and b2-AR [55]. However, E may stimulate b3-ARinduced adenylate-cyclase activity [4] and dose-dependently activate lipolysis in vivo [13]. Accordingly, the mechanism which we hypothesize for the effects of TLQP-21 on adipose tissue is the following: TLQP-21 acting centrally (mechanism still to be determined) would decrease sympathetic tone, resulting in overall decreased NE release in the eWAT nerve endings, thus determining an up-regulation of b3-AR (and to a lower extent also of b1-AR and b2-AR); additionally, TLQP-21 would determine increased E release from chromaffin cells in the adrenal medulla [of note, a recent study identified TLQPimmunostaining in E-but not in NE-chromaffin cells in the adrenal medulla of bovine, swine and rat [23]; the net result would be increased E-stimulated b-AR mediated catabolic effects in the fat pads and resulting resistance to obesity.…”
Section: Discussionmentioning
confidence: 99%
“…b3-AR are more stimulated by local NE and less by E than for example b2-AR and this is due to the reduced affinity to E of b3-AR compared to b1-and b2-AR [55]. However, E may stimulate b3-ARinduced adenylate-cyclase activity [4] and dose-dependently activate lipolysis in vivo [13]. Accordingly, the mechanism which we hypothesize for the effects of TLQP-21 on adipose tissue is the following: TLQP-21 acting centrally (mechanism still to be determined) would decrease sympathetic tone, resulting in overall decreased NE release in the eWAT nerve endings, thus determining an up-regulation of b3-AR (and to a lower extent also of b1-AR and b2-AR); additionally, TLQP-21 would determine increased E release from chromaffin cells in the adrenal medulla [of note, a recent study identified TLQPimmunostaining in E-but not in NE-chromaffin cells in the adrenal medulla of bovine, swine and rat [23]; the net result would be increased E-stimulated b-AR mediated catabolic effects in the fat pads and resulting resistance to obesity.…”
Section: Discussionmentioning
confidence: 99%
“…LPL activity was measured using labeled triolein -3H as a substrate, according to the modified methods of Ballart et al (2003). LPL, HL, SDH and MDH activities are expressed as U (units) per mg of soluble protein (specific activity).…”
Section: Sampling and Analytical Methodsmentioning
confidence: 99%
“…The homogenates were centrifuged for 5 min at 4°C and then the supernatants were collected for enzyme analysis. SDH was analyzed as described by Slater and Bonner (1952), LDH by Jones and Sidell (1982), MDH by Ochoa (1955), LPL by (Ballart et al, 2003), HL following Ehnholm et al (1975) modified by Burgaya et al (1989), Mn-SOD by De Rosa et al (1980). All enzyme activities were expressed as U (units) per mg or g of soluble protein.…”
Section: Sampling and Analysismentioning
confidence: 99%