Isoprenoid quinone content and cellular fatty acid composition of Campylobacter species

Moss, C W; Kai, Akemi; Lambert, M A; Patton, C M

doi:10.1128/jcm.19.6.772-776.1984

Cited by 50 publications

(39 citation statements)

References 18 publications

(29 reference statements)

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“…Cells from five HIAB plates were hydrolyzed by adding 0.2 ml of 50% aqueous KOH and 3 ml of 1% pyrogallol in methanol and heating them at 100°C for 10 min. After being cooled to room temperature, the quinones were extracted and examined by reverse-phase high-performance liquid chromatography as described previously (7,18). Tentative identification was established by comparing retention times with authentic standards supplied by Hoffmann-La Roche Co. (Basel, Switzerland).…”

Section: Methodsmentioning

confidence: 99%

Francisella philomiragia comb. nov. (formerly Yersinia philomiragia) and Francisella tularensis biogroup novicida (formerly Francisella novicida) associated with human disease

et al. 1989

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Over a 12-year period, 16 human strains of a gram-negative, catalase-positive, halophilic, aerobic, nonmotile, small coccoid bacterium were received for identification. On the bases of biochemical characteristics and cellular fatty acid profiles, 14 of these strains were similar to the "Philomiragia" bacterium (Yersinia philomiragia, species incertae sedis). Additional characteristics were growth on Thayer-Martin agar but no growth or sparse, delayed growth on MacConkey agar; oxidase positive; acid production, often weak and delayed, from D-glucose, sucrose, and maltose; urease negative; no reduction of nitrates; and H2S produced but often delayed in triple sugar iron agar. Both the human isolates and the "Philomiragia" bacterium contained C10:0, C14:0e C16:0, C,8.1 w9c, C18:0, 3-OH C18:09 C22:0, and C24:1 as major cellular fatty acids and ubiquinone eight (Q8) as the major isoprenoid quinone. These cellular acids in these relative amounts have been found previously only in Francisella tularensis and Francisella novicida, suggesting a relationship between the "Philomiragia" bacterium and Francisella species. Of the 14 human "Philomiragia"-like isolates, 9 were from blood, 3 were from lung biopsies or pleural fluid, and one each was from peritoneal fluid and cerebrospinal fluid. DNA relatedness studies (hydroxyapatite method, 50 and 65°C) showed that these 14 strains were a single group that was the same species as the "Philomiragia" bacterium. Two other human strains were oxidase negative and H2S negative. They formed a single DNA relatedness group that was indistinguishable from the type strains of both F. tularensis and F. novicida. DNA relatedness of "Philomiragia" bacterium type and other strains to strains of F. novicida and F. tularensis, including the type strains, was 35 to 46%. One of the two F. novicidaand F. tularensis-like strains was isolated from blood, and the other was isolated from a cervical lymph node. On the basis of these findings, we propose transferring Y. philomiragia from the genus Yersinia to the genus Francisella as Francisella philomiragia comb. nov. Having confirmed that F. novicida and F. tularensis are the same species and having shown that F. novicida is pathogenic for humans, we further propose eliminating the species F. novicida and demoting it to a biogroup of F. tularensis.

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Section: Methodsmentioning

confidence: 99%

Francisella philomiragia comb. nov. (formerly Yersinia philomiragia) and Francisella tularensis biogroup novicida (formerly Francisella novicida) associated with human disease

et al. 1989

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“…Cells were removed by adding 1 ml of distilled water to each plate and gently removing growth with a glass rod. Fatty acid methyl esters were prepared, extracted, and analyzed by capillary gas-liquid chromatography as previously described (10,12).…”

Section: Methodsmentioning

confidence: 99%

Human disease associated with "Campylobacter upsaliensis" (catalase-negative or weakly positive Campylobacter species) in the United States

et al. 1989

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Catalase-negative or weakly positive (CNW) thermotolerant campylobacteria, first isolated from dogs in 1983, were recently recognized as a new species, "Campylobacter upsaliensis," but their association with human illness has not been established. Twelve human isolates received at the Centers for Disease Control between 1980 and 1986 were identified as CNW campylobacteria by biochemical tests, cellular fatty acid composition, and antimicrobial susceptibility patterns. Eleven CNW Campylobacter strains tested by DNA-DNA hybridization (hydroxyapatite method) were all highly related and were related to two "C. upsaliensis" strains at the species level (86% under optimal conditions and 76% under stringent conditions). Clinical information was obtained for Il human isolates from three stool and eight blood specimens. They were isolated from four female and seven male patients 6.5 months to 83 years of age residing in 10 different states. The patients had a wide spectrum of illnesses. The stool isolates were obtained from two previously healthy persons during episodes of acute gastroenteritis and from one immunocompromised patient with persistent diarrhea and fever. The blood isolates were obtained from two infants with fever and respiratory symptoms; a young woman with a ruptured ectopic pregnancy; three elderly men with underlying chronic diseases; and two immunocompromised adults. In a bactericidal assay to assess sensitivity to serum, seven of eight blood isolates showed some resistance to killing by pooled normal human serum. These observations suggest that "C. upsaliensis" is a potential human pathogen associated with both gastroenteritis and bacteremia in normal hosts and with opportunistic infection in immunocompromised individuals.

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“…The study of the respiratory quinone components of campylobacters was of major importance for the revision of their classification. The respiratory chain of campylobacters, unlike most gram-negative bacteria, comprises only menaquinones (37,38,114,217,218,316). All species belonging to the Campylobacter rRNA homology group, including the former W. recta, W. curva, B. gracilis, and B. ureolyticus, contain menaquinone-6 and the so-called thermoplasmaquinon (a methyl-substituted menaquinone-6) as major components.…”

Section: Polyphasic Taxonomy Of the Genus Campylobactermentioning

confidence: 99%

“…Cellular fatty acid analysis. Several authors studied the suit-ability of cellular fatty acid analysis for the differentiation and identification of campylobacters (18,44,53,115,175,181,217,342). Lambert et al (175) and Goodwin et al (115) used similar procedures and performed the most comprehensive studies.…”

Section: Polyphasic Taxonomy Of the Genus Campylobactermentioning

confidence: 99%

Polyphasic taxonomy, a consensus approach to bacterial systematics.

Vandamme

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Gillis

et al. 1996

Microbiological Reviews

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Isoprenoid quinone content and cellular fatty acid composition of Campylobacter species

Cited by 50 publications

References 18 publications

Francisella philomiragia comb. nov. (formerly Yersinia philomiragia) and Francisella tularensis biogroup novicida (formerly Francisella novicida) associated with human disease

Francisella philomiragia comb. nov. (formerly Yersinia philomiragia) and Francisella tularensis biogroup novicida (formerly Francisella novicida) associated with human disease

Human disease associated with "Campylobacter upsaliensis" (catalase-negative or weakly positive Campylobacter species) in the United States

Polyphasic taxonomy, a consensus approach to bacterial systematics.

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