Isoprenoid quinone, cellular fatty acid composition and diaminopimelic acid isomers of newly classified thermophilic anaerobic Gram-positive bacteria

Abstract

The configuration of quinone systems, cellular fatty acids and diaminopimelic acids in 11 species of thermophilic clostridia which have been classified into new genera based on their 16S rRNA sequences was determined. It was found that menaquinone 7 was present in 10 species as the major component of menaquinone systems by analyses using HPLC with photodiode-array detector and electron impact mass spectrometry. In seven of the 11 species, the major cellular fatty acids were determined to be iso-15:0 and iso-17:0. As a results of chemotaxonomic studies it was demonstrated that the representatives of the new genera Thermoanaerobacter and Thermoanaerobacterium were heterogeneous in their chemical composition, whereas strains of the new genus Moorella and of the new unnamed genus which is composed of (Clostridium) stercorarium and (Clostridium) thermolacticum showed homogeneity in their chemotaxonomic characteristics. z 1998 Federation of European Microbiological Societies. Published by Elsevier Science B.V.

The use of fatty acid methyl esters as biomarkers to determine aerobic, facultatively aerobic and anaerobic communities in wastewater treatment systems

“…The analysis of fatty acid methyl esters (FAME) profiles provides insight into important changes in microbial communities in wastewater treatment plants, in such a way as to avoid the microbial culture bias. FAME analysis may be used to identify isolated and pure microbial cultures (Sasser, 1997), in taxonomic studies (Yamamoto et al, 1998) or for the identification of bacteria of medical importance (McNabb et al, 1997).…”

Genome-Based Comparison of All Species of the Genus Moorella, and Status of the Species Moorella thermoacetica and Moorella thermoautotrophica

“…Due to the observation of conflicting phenotypic traits that have been connected with the two different species, the scientific community has already questioned the taxonomic status of the two species M. thermoautotrophica and M. thermoacetica (Carlier and Bedora-Faure, 2006;Kimura et al, 2016). In addition to the high similarity of the genomes' 16S rRNA gene sequence, there are further similarities described for M. thermoacetica/thermoautotrophica strains such as a similar fatty acid and peptidoglycan profile (Yamamoto et al, 1998) and presence of the same menaquinone (Das et al, 1989). However, these features are generally conserved in "closely related" taxa and one would not expect significant differences between strains showing such a high degree of genetic similarity (Tindall, unpublished).…”

“…Based on DNA-DNA hybridization experiments and 16S rRNA gene sequence analysis, Kimura et al (2016) concluded that the species M. thermoautotrophica should be reclassified as M. thermoacetica. Over time, phenotypic differences between M. thermoacetica and M. thermoautotrophica were described, but often with partly conflicting results (Cato et al, 1986;Das et al, 1989;Yamamoto et al, 1998;Carlier and Bedora-Faure, 2006).…”