Genome-Based Comparison of All Species of the Genus Moorella, and Status of the Species Moorella thermoacetica and Moorella thermoautotrophica

Redl, Stephanie Maria Anna; Esser, Carola; Bengelsdorf, Frank R.; Jensen, Troy; Jendresen, Christian Bille; Tindall, Brian J.; Daniel, Rolf; Dürre, Peter; Nielsen, Alex Toftgaard

doi:10.3389/fmicb.2019.03070

Cited by 16 publications

(11 citation statements)

References 71 publications

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“…In this context, it is interesting that T. kivui , despite sharing high 16 S sequence similarity to other Thermoanaerobacter species, is the only acetogenic isolate of the genus so far. This is in contrast to another thermophilic acetogenic genus Moorella , for which multiple closely related acetogenic strains have been isolated independently [ 41 ]. Since most acetogens encode the genes of the Wood-Ljungdahl pathway in a single large gene cluster, and T. kivui ’s WLP genes are even more concentrated than most [ 42 ], it seems plausible that its acetogenic capabilities could result from an HGT event.…”

Section: Resultsmentioning

confidence: 99%

DNA uptake from a laboratory environment drives unexpected adaptation of a thermophile to a minor medium component

Zeldes

Jain

Baum

et al. 2023

ISME Communications

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DNA uptake is widespread among microorganisms and considered a strategy for rapid adaptation to new conditions. While both DNA uptake and adaptation are referred to in the context of natural environments, they are often studied in laboratories under defined conditions. For example, a strain of the thermophile Thermoanaerobacter kivui had been adapted to growth on high concentrations of carbon monoxide (CO). Unusual phenotypes of the CO-adapted strain prompted us to examine it more closely, revealing a horizontal gene transfer (HGT) event from another thermophile, Thermoanaerobacter sp. strain X514, being cultured in the same laboratory. The transferred genes conferred on T. kivui the ability to utilize trehalose, a trace component of the yeast-extract added to the media during CO-adaptation. This same HGT event simultaneously deleted a native operon for thiamine biosynthesis, which likely explains why the CO-adapted strain grows poorly without added vitamins. Attempts to replicate this HGT by providing T. kivui with genomic DNA from Thermoanaerobacter sp. strain X514 revealed that it is easily reproducible in the lab. This subtle form of “genome contamination” is difficult to detect, since the genome remains predominantly T. kivui, and no living cells from the original contamination remain. Unexpected HGT between two microorganisms as well as simultaneous adaptation to several conditions may occur often and unrecognized in laboratory environments, requiring caution and careful monitoring of phenotype and genotype of microorganisms that are naturally-competent for DNA uptake.

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Section: Resultsmentioning

confidence: 99%

DNA uptake from a laboratory environment drives unexpected adaptation of a thermophile to a minor medium component

Zeldes

Jain

Baum

et al. 2023

ISME Communications

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“…Redl et al [19] re-isolated two different strains from cultures of DSM 1974 T , which were deposited as DSM 103284 and DSM 103132. Both strains were genomically studied (with the genome accession numbers CP017019 and CP017237) and closely affiliated with all other members of the species Moorella thermoacetica .…”

Section: Opinion 115mentioning

confidence: 99%

“…The fact that Redl et al [19] did not recover the OTU-4 [15] led to their conclusions that DSM 1974 T was a mixed culture of two different strains of Moorella thermoacetica . However, a larger effort may be needed to retrieve the OTU-4 representatives, which had been readily isolated previously from the deposits of both collections DSMZ and ATCC.…”

Section: Opinion 115mentioning

confidence: 99%

“…the second clone affiliated with Moorella humiferrea, which was obtained from both DSM 1974 T and ATCC 33924 T cultures by Kimura et al [15]. Redl et al [19] reached the conclusion that it is impossible to determine whether the original strain was already a mixture of different organisms or was mixed (contaminated) after its deposit. These authors recommended the recognition of Moorella thermoautotrophica (Wiegel et , it is not of primary importance whether JW 701/3 T was already a mixed culture prior to its deposit at DSMZ.…”

Section: Opinion 115mentioning

confidence: 99%

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Judicial Opinions 112–122

Arahal

Busse

Bull

et al. 2022

International Journal of Systematic and Evolutionary Microbiology

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Opinion 112 denies the request to place Seliberia Aristovskaya and Parinkina 1963 (Approved Lists 1980) on the list of rejected names because the information provided is insufficient. For the same reason, Opinion 113 denies the request to reject Shewanella irciniae Lee et al. 2006 and Opinion 114 denies the request to reject the name Enterobacter siamensis Khunthongpan et al. 2014. Opinion 115 rejects the epithet of Moorella thermoautotrophica (Wiegel et al. 1981) Collins et al. 1994, which is regarded as a nomen confusum. To assess the consequences of Rule 8, Opinion 116 revisits names of taxa above the rank of genus which should comprise the stem of the name of a nomenclatural type and a category-specific ending but fail to do so. Such names should be orthographically corrected if the sole error is the inadvertent usage of an incorrect stem or be regarded as illegitimate if otherwise. The necessary corrections are made for a number of names. In Opinion 117, the request to designate Methylothermus subterraneus Hirayama et al. 2011 as the type species of the genus Methylothermus is denied because an equivalent action compatible with the Code was already conducted. In Opinion 118, the possible orthographical correction of the name Flaviaesturariibacter is treated, as are the analogous cases of Fredinandcohnia and Hydrogeniiclostidium . The genus names are corrected to Flaviaestuariibacter, Ferdinandcohnia and Hydrogeniiclostridium , respectively. Opinion 119 concludes that assigning Actinomycetales Buchanan 1917 (Approved Lists 1980) as nomenclatural type of the class Actinobacteria Stackebrandt et al. 1997 would not render that name legitimate if Rule 8 remained retroactive. The request is granted but Actinomycetales is also assigned as type of Actinomycetes Krassilnikov 1949 (Approved Lists 1980). In Opinion 120, the possible orthographical correction of the name Amycolatopsis albidoflavus is treated. It is grammatically corrected to Amycolatopsis albidoflava. Six names which could according to Rule 61 be grammatically corrected by anyone are also corrected. Opinion 121 denies the request to revise Opinion 69 and notes that Opinion 69 does not have the undesirable consequences emphasized in the request. In Opinion 122, the request to reject various taxon names of Mollicutes proposed in 2018 is denied because it is based on misinterpretations of the Code, which are clarified. Alternative ways to solve the perceived problems are outlined. These Opinions were ratified by the voting members of the International Committee on Systematics of Prokaryotes.

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“…Interest to acetogenic bacteria has increased within recent years due to their potential ability to produce acetate from syngas. Thermophilic acetogens are of significance, since their use would reduce gas cooling requirements, allow for cost-efficient recovery of products with a relatively low boiling point, and decrease the risk of contamination [13].…”

Section: Introductionmentioning

confidence: 99%

Thermophilic sulfate-reducing bacteria Moorela thermoacetica Nadia-3, isolated from “Nadiia” pit spoil heap of Chervonohrad mining region

2021

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Introduction. Thermophilic sulfate-reducing bacteria attract attention of scientists as the potential agents of purification of wastewater polluted by sulfur and its compounds, heavy metal ions and organic compounds. These bacteria oxidize different organic substrates using metals with variable valency as electron acceptors and transform them into non-toxic or less toxic forms for living organisms. However, wastewater contains high concentrations of different toxic xenobiotics, particularly, metal ions that have negative influence on living organisms. For this reason, it is important to use resistant strains of microorganisms for the purification of wastewater. The aim of this work was to identify the thermophilic sulfur-reducing bacteria, isolated from “Nadiia” pit spoil heap of Chervonohrad mining region, and to study their properties. Materials and Methods. Thermophilic sulfur-reducing bacteria were isolated from the samples of rock of “Nadiia” pit heap at 50 cm depth. Bacteria were cultivated in TF medium under the anaerobic conditions in anaerostates. Cell biomass was measured turbidimetrically using the photoelectric colorimeter KFK-3 (λ = 340 nm, 3 mm cuvette). Hydrogen sulfide content was measured photoelectrocolorymetrically by the production of methylene blue. Organic acids content was measured by high performance liquid chromatography. Cr(VI), Fe(III), Мn(IV) and NO3– content was measured turbidimetrically. Results. Thermophilic sulfur-reducing bacteria were isolated from the rock of “Nadiia” pit heap of Chervonohrad mining region. They were identified as Moorela thermoacetica based on the morpho-physiological and biochemical properties and on the results of phylogenetic analysis. M. thermoacetica Nadia-3 grow in the synthetic TF medium, have the shape of elongated rods, are gram-positive, endospore-forming. They form light brown colonies. Optimal growth was observed at 50–55 °C, pH 6.5–7. The bacteria utilize glucose, starch, fructose, maltose, lactose, sodium lactate, arabinose, cellulose, maltose, glycerol, fumarate, and ethanol as carbon sources. The highest sulfidogenic activity of M. thermoacetica Nadia-3 was found in media with glycerol, lactose, and glucose. M. thermoacetica Nadia-3 reduce SO42-, S2O32-, Fe(III), NO3–, Cr(VI) compounds besides elemental sulfur. They accumulate biomass at K2Cr2O7 concentrations of 0.1–1 mM. Sulfur reduction is not the main way of energy accumulation. Conclusions. Thermophilic chromium-resistant sulfur-reducing bacteria M. thermoacetica Nadia-3, that produce hydrogen sulfide during the oxidation of different organic compounds, were isolated from the rock of “Nadiia” pit heap. They reduce Fe(III), Cr(VI), NO3–, SO42-, S2O32-, besides elemental sulfur.

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Genome-Based Comparison of All Species of the Genus Moorella, and Status of the Species Moorella thermoacetica and Moorella thermoautotrophica

Cited by 16 publications

References 71 publications

DNA uptake from a laboratory environment drives unexpected adaptation of a thermophile to a minor medium component

DNA uptake from a laboratory environment drives unexpected adaptation of a thermophile to a minor medium component

Judicial Opinions 112–122

Thermophilic sulfate-reducing bacteria Moorela thermoacetica Nadia-3, isolated from “Nadiia” pit spoil heap of Chervonohrad mining region

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