Isoorientin from Gypsophila elegans induces apoptosis in liver cancer cells via mitochondrial-mediated pathway

Lin, Xiaobo; Wei, Jinbin; Chen, Yongxin; He, Ping; Lin, Jinxiu; Tan, Sheng; Nie, Jinlan; Lu, Sheng; He, Min; Lu, Zhaojun; Huang, Quanfang

doi:10.1016/j.jep.2016.04.050

Cited by 53 publications

(25 citation statements)

References 25 publications

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“…Total RNA was extracted by TRIzol reagent (Life Technologies, Inc.) according to the manufacturer’s instructions, and RT-PCR assay was performed as described in our previous study [19]. The primers used in this study were as follows: NF-κB: 5′-GTT ATC GTT CAG TTG GTC ACA-3′ and 5′-ATA TGC CGT CCT CAC AGT-3′; Atg5: 5′-GGC CAT CAA TCG GAA ACT CAT-3′ and 5′-CAG CCA CAG GAC GAA ACA GC-3′; Atg7: 5′-GGC TCA TCG CTT TTT GCC A AC ATC-3′ and 5′-GCA CTG CTG GTC CAA GGT CCG-3′; GAPDH: 5’-CGC CTG CTT CAC CAC CTT-3’ and 5’-CCT GCC GCC TGG AGA AA-3’.…”

Section: Methodsmentioning

confidence: 99%

Trolline Ameliorates Liver Fibrosis by Inhibiting the NF-κB Pathway, Promoting HSC Apoptosis and Suppressing Autophagy

Bai¹,

Huang²,

Nie³

et al. 2017

Cell Physiol Biochem

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Background/Aims: Previous studies have shown that trolline possesses various forms of pharmacological activity, including antibacterial and antiviral potency. The present paper addressed the putative hepatoprotective effects of trolline. Methods: Rats received 2 ml/kg CCl₄ (mixed 1: 1 in peanut oil) intragastrically twice a week for 8 weeks to induce hepatic fibrosis. The animals were then treated with trolline for additional 4 weeks. Liver pathology and collagen accumulation were observed by hematoxylin-eosin and Masson’s trichrome staining, respectively. Serum transaminase activity and collagen-related indicator level were determined by commercially available kits. NF-κB pathway activation was also examined. Moreover, the effects of trolline on hepatic stellate cell (HSC-T6) apoptosis, mitochondrial membrane potential (MMP), and autophagy were assessed. Results: Trolline significantly alleviated CCl₄-induced liver injury and notably reduced the accumulation of collagen in liver tissues. Trolline treatment also markedly decreased inflammatory cytokines levels by inhibiting the NF-κB pathway. Trolline strongly inhibited HSC-T6 activation and notably induced cell apoptosis by modulating the Bax/Bcl-2 ratio, caspase activity, and MMP. Moreover, trolline significantly inhibited HSC-T6 autophagy, as evidenced by the decrease in the formation of autophagic vacuoles and the number of autophagosomes, by regulating the expression levles of LC3, Beclin-1, P62, Atg 5 and 7. Conclusion: Our study demonstrates that trolline ameliorates liver fibrosis, possibly by inhibiting the NF-κB pathway, promoting HSCs apoptosis and suppressing autophagy.

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Section: Methodsmentioning

confidence: 99%

Trolline Ameliorates Liver Fibrosis by Inhibiting the NF-κB Pathway, Promoting HSC Apoptosis and Suppressing Autophagy

Bai¹,

Huang²,

Nie³

et al. 2017

Cell Physiol Biochem

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“…The Consulting the literature showed that the phenolic acid components chlorogenic acid (Xue et al, 2017;Shao, Zhang, Chen, & Sun, 2015) and caffeic acid (Brautigan, Gielata, Heo, Kubicka, & Wilkins, 2018), and the flavonoids scutellarin (Nie et al, 2018;Sun et al, 2018;Tang & Gao, 2019), isoorientin (Huang, Jin et al, 2018;Lin et al, 2016), isovitexin (Lv, 2018), scutellarein (Jiang et al, 2018;Lu, Li, Li, & Asia, 2017;Zheng et al, 2017), luteolin (Niu, Guo, Gan, Bao, & Ren, 2015) and apigenin (Bhattacharya et al, 2018;Masuelli et al, 2017;Cai et al, 2011) had different degrees of inhibition on tumor cells. Among them, caffeic acid, isoorientin, isovitexin, luteolin and apigenin had better antiliver tumor activities.…”

Section: Analysis Of Migration Components In Plasmamentioning

confidence: 99%

Analysis of plasma migration components in Patrinia villosa (Thunb.) Juss. effective parts by UPLC–Q‐TOF–MS

Han

Wang

Yang

et al. 2019

Biomedical Chromatography

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Patrinia villosa (Thunb.) Juss. (PVJ) is described as pungent, bitter and slightly cold in Chinese medicine, and is associated with the large intestine, stomach and liver meridians. The preliminary experiments of our research team proved that PVJ total flavonoids have excellent inhibitory effects on liver cancer cells. The present experiment uses the UPLC–Q‐TOF–MS technology and serum pharmacochemistry methods to analyze the chemical components in vitro and in vivo of PVJ antiliver tumors. A total of 14 chemical components were identified in the total flavonoids extract of PVJ, and it is mainly composed of flavonoids, flavonones, flavonols and phenolic acids. At the same time, seven prototypical components and seven metabolic components were detected in the drug‐containing plasma. Hydrocaffeate and scutellarein are the phase I metabolites of caffeic acid and scutellarin, respectively. Sulfated apigenin, sulfated luteolin, sulfated kaempferol and methylated kaempferol are the II phase metabolites of apigenin, luteolin, kaempferol, respectively. The experiment provides a reference for the research and development of antitumor drug candidates, and provides a basis for revealing the bioactive components of PVJ and the antitumor mechanism.

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“…Apoptosis is a key cell survival process when it inhibits only tumor cells. 37 Therefore, promoting apoptosis has become the focus of anticancer research.…”

Section: In Vitro Cell Apoptosis Studiesmentioning

confidence: 99%

Co-delivery doxorubicin and silybin for anti-hepatoma via enhanced oral hepatic-targeted efficiency

Liu

Yang

Wang

et al. 2018

IJN

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BackgroundTo establish the combination of doxorubicin (DOX) and silybin (SLB) in oral hepatic-targeting liposomes with the goal of reducing cardiotoxic side effects and improve oral hepatoma treatment.MethodsDistearoylphosphatidylethanolamine–polyethylene glycol–cholic acid-modified liposomes (CA-LP) were used to encapsulate DOX and SLB (CA-LP–DOX/SLB), and the hepatic targeting, efficacy against hepatoma and cardioprotective effects were evaluated by cell toxicity, scratch and apoptosis in vitro studies, and pharmacokinetics and pharmacodynamics in vivo studies.ResultsIn vitro cell studies showed that CA-LP–DOX/SLB inhibited HepG2 cell proliferation and HCC97H cell migration, and protected H9c2 cells. In vivo pharmacokinetics demonstrated that the CA-LP–DOX/SLB-treated group showed higher liver accumulation and lower heart accumulation of DOX relative to those in the CA-LP–DOX and LP–DOX-treated groups. In vivo pharmacodynamic studies showed that the CA-LP–DOX/SLB-treated group not only efficiently inhibited growth but also induced significantly less tissue damage than that observed in the CA-LP–DOX-treated group.ConclusionConcurrent administration of DOX and SLB via CA-LP provided a viable strategy to mitigate acute DOX-induced cardiotoxicity.

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Isoorientin from Gypsophila elegans induces apoptosis in liver cancer cells via mitochondrial-mediated pathway

Cited by 53 publications

References 25 publications

Trolline Ameliorates Liver Fibrosis by Inhibiting the NF-κB Pathway, Promoting HSC Apoptosis and Suppressing Autophagy

Trolline Ameliorates Liver Fibrosis by Inhibiting the NF-κB Pathway, Promoting HSC Apoptosis and Suppressing Autophagy

Analysis of plasma migration components in Patrinia villosa (Thunb.) Juss. effective parts by UPLC–Q‐TOF–MS

Co-delivery doxorubicin and silybin for anti-hepatoma via enhanced oral hepatic-targeted efficiency

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