Isoniazid promotes the anti-inflammatory response in zebrafish associated with regulation of the PPARγ/NF-κB/AP-1 pathway

Zhang, Yun; Wang, Chao; Jia, Zhiyan; Ma, Ruijiao; Wang, Xuefang; Chen, Weiyun; Liu, Ke-chun

doi:10.1016/j.cbi.2019.108928

Cited by 31 publications

(18 citation statements)

References 36 publications

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“…Zhang et al (Y. Zhang et al, 2020 ). utilized the inflammation model of zebrafish induced by CuSO 4 to study the anti-inflammatory mechanism of isoniazid.…”

Section: Discussionmentioning

confidence: 99%

An Integrated Strategy for Rapid Discovery and Identification of Quality Markers in Gardenia Fructus Using an Omics Discrimination-Grey Correlation-Biological Verification Method

et al. 2021

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Background: Gardenia Fructus (GF), a traditional Chinese medicine of Gardenia Ellis in Rubiaceae family, has the potential to clear heat and purge fire and has been widely used to treat multiple infection-related diseases. However, the quality markers (Q-Markers) of GF have not been revealed comprehensively.Methods: In this experiment, the transgenic zebrafish lines, Tg (l-fabp:EGFP) and Tg (lyz:EGFP), were used to evaluate two main kinds of traditional efficacies of GF, hepatoprotective and anti-inflammatory effects. All the GF samples from different production areas were tested their anti-liver injury and anti-inflammantory activities. High-performance liquid chromatography-quadrupole time-of-flight mass spectrometry method (HPLC-Q-TOF/MS) was employed for herbal metabonomic analysis of GF samples. Gray correlation analysis (GCA) was utilized to screen out the components closely associated with the activities. Finally, the zebrafish model was applied to verify the bioactivity of the crucial components to determine the Q-Markers of GF.Results: The zebrafish models were established by inducing with hydrogen peroxide or copper sulfate and applied to quickly evaluate the hepatoprotective effect and inflammation of GF samples. 27 potentially active components for liver protection and 21 potentially active components with anti-inflammatory properties were identified by herbal metabolomic analysis based on HPLC-Q-TOF/MS. The GCA result showed that five of the 27 components were highly correlated with liver protection, 15 of the 21 components were highly correlated with anti-inflammatory activity. Among them, geniposide and crocin-1 were confirmed their bioactivities on zebrafish experiment to be responsible for the protective effects of GF against liver injury, and genipin-1-β-D-gentiobioside, quinic acid, gardenoside, d-glucuronic acid, l-malic acid, mannitol, rutin, and chlorogenic acid were confirmed to be responsible for the anti-inflammatory effects. Finally, according to the screening principles of Q-Markers, genipin-1-β-D-gentiobioside, geniposide, and gardenoside were preliminarily identified to be the Q-Markers of GF.Conclusion: This study established an effective research strategy of “Omics Discrimination-Grey Correlation-Biological Verification,” which enabled the rapid identification of key pharmacological components of GF. These markers have provided a scientific basis for constructing a modern quality evaluation system for GF.

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“…Zhang et al (Y. Zhang et al, 2020 ). utilized the inflammation model of zebrafish induced by CuSO 4 to study the anti-inflammatory mechanism of isoniazid.…”

Section: Discussionmentioning

confidence: 99%

An Integrated Strategy for Rapid Discovery and Identification of Quality Markers in Gardenia Fructus Using an Omics Discrimination-Grey Correlation-Biological Verification Method

et al. 2021

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“…until 72 hpf). Zebrafish embryos were further treated with CuSO4 (20 µM) for 1 h to induce the acute inflammation, by adopting the previously reported protocol ( 26 , 27 ). Following treatment, embryos were fixed at room temperature for 2 h in 4% paraformaldehyde, and macrophage migration to the lateral line was examined using fluorescent microscopy (magnification, x100).…”

Section: Methodsmentioning

confidence: 99%

“…Breeding was set up in the Animal Unit at Glasgow Caledonian University using adult wild-type and transgenic zebrafish (Tg:zlyz-EGFP) expressing EGFP in primitive macrophages to get fertilized embryos, which were maintained in E3 medium consisting of 5 mM NaCl, 0.17 mM KCl, 0.33 mM CaCl 2 and 0.33 mM MgSO 4 (25). CuSO 4 has been used to induce acute inflammation, as previously described (26)(27)(28). A total of 24 transgenic zebrafish (Tg:zlyz-EGFP) embryos at 48 h post-fertilization (hpf) were treated with or without GJE (diluted in E3 medium, 5 µg/ml) for 24 h (i.e.…”

Section: High-performance Liquid Chromatography (Hplc) Analysismentioning

confidence: 99%

“…Additionally, the embryos were collected for further gene expression analysis. Lipopolysaccharides (LPS) have also been commonly used to induce inflammation in zebrafish (26)(27)(28)(29), and this method was also used in the present study. Wild-type zebrafish embryos at 48 hpf were treated with GJE (5 µg/ml) only, or with GJE plus LPS (5 µg/ml) for 24 h. Following the treatment, embryos were collected and subjected to gene expression analysis.…”

Section: High-performance Liquid Chromatography (Hplc) Analysismentioning

confidence: 99%

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Anti‑inflammatory activities of Gardenia jasminoides extracts in retinal pigment epithelial cells and zebrafish embryos

et al. 2021

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Age-related macular degeneration (AMD) is the most common cause of visual impairment in developed countries. Inflammation serves a critical role in the pathogenesis of AMD. Gardenia jasminoides is found in several regions of China and is traditionally used as an organic yellow dye but has also been widely used as a therapeutic agent in numerous diseases, including inflammation, depression, hepatic and vascular disorders, which may reflect the variability of functional compounds that are present in Gardenia jasminoides extracts (GJE). To investigate the therapeutic potential of GJE for AMD, ARPE-19 cells were treated with lipopolysaccharide (LPS) or LPS plus GJE. GJE significantly decreased LPS-induced expression of proinflammatory cytokines, including IL-1β, IL-6 and TNF-α. In the in vivo study, GJE inhibited CuSO4-induced migration of primitive macrophages to the lateral line in zebrafish embryos. GJE also attenuated expression of cytokines (IL-1β, IL-6 and TNF-α), NFKB activating protein ( nkap ) and TLR4 in ARPE-19 cells. The results of the present study demonstrated the anti-inflammatory potential of GJE in vitro and in vivo , and suggested GJE as a therapeutic candidate for AMD.

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“…It was observed that in recent years, isoniazid and its derivatives have received attention and are being widely studied. The derivatives of isoniazid are evaluated for their various biological activities, such as anti-inflammatory [8], anticancer [9,10], antimicrobial [11][12][13], anti-tubercular [2,11,[14][15][16][17], and in the treatment of Alzheimer's disease [18].…”

Section: Introductionmentioning

confidence: 99%

Microwave-Assisted Synthesis of Schiff Bases of Isoniazid and Evaluation of Their Anti-Proliferative and Antibacterial Activities

Al-Hiyari

Shakya

Naik

et al. 2021

Molbank

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Three new Schiff bases of isoniazid were synthesized using microwave-assisted synthesis and conventional condensation with aromatic aldehydes. Synthesized compounds were characterized using elemental analysis, IR, NMR, and Mass spectroscopy. Synthesized compounds were evaluated for antiproliferative activity against MCF-7 cell line. The IC50 values were from 125 to 276 µM. The compounds were also evaluated for antibacterial activity against Staphylococcus aureus and Escherichia coli. Results showed that the synthesized compounds produce significant antibacterial activity in vitro. Inhibition of compounds ranged from 13 to 18 mm.

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Isoniazid promotes the anti-inflammatory response in zebrafish associated with regulation of the PPARγ/NF-κB/AP-1 pathway

Cited by 31 publications

References 36 publications

An Integrated Strategy for Rapid Discovery and Identification of Quality Markers in Gardenia Fructus Using an Omics Discrimination-Grey Correlation-Biological Verification Method

An Integrated Strategy for Rapid Discovery and Identification of Quality Markers in Gardenia Fructus Using an Omics Discrimination-Grey Correlation-Biological Verification Method

Anti‑inflammatory activities of Gardenia jasminoides extracts in retinal pigment epithelial cells and zebrafish embryos

Microwave-Assisted Synthesis of Schiff Bases of Isoniazid and Evaluation of Their Anti-Proliferative and Antibacterial Activities

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