Isoliquiritigenin inhibits IκB kinase activity and ROS generation to block TNF-α induced expression of cell adhesion molecules on human endothelial cells

Kumar, Sarvesh; Sharma, Amit; Madan, Babita; Singhal, Vandana; Ghosh, Balaram

doi:10.1016/j.bcp.2007.01.015

Cited by 110 publications

(76 citation statements)

References 35 publications

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“…Nuclear extracts were prepared from Jurkat cells as previously described (33). These extracts were incubated with radio-labeled double stranded oligonucleotide probes for hsa-miR-106a potential promoter region.…”

Section: Rna Extraction and Rt-pcrmentioning

confidence: 99%

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Posttranscriptional regulation of interleukin-10 expression by hsa-miR-106a

Sharma

Kumar

Aich

et al. 2009

Proc. Natl. Acad. Sci. U.S.A.

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IL-10 is a key regulator of the immune system that critically determines health and disease. Its expression is finely tuned both at the transcriptional and posttranscriptional levels. Although the importance of posttranscriptional regulation of IL-10 has been previously shown, understanding the underlying mechanisms is still in its infancy. In this study, using a combination of bioinformatics and molecular approaches, we report that microRNA (hsamiR-106a) regulates IL-10 expression. The hsa-miR-106a binding site in the 3 UTR of IL10 has been identified by site-directed mutagenesis studies. Also, the involvement of transcription factors, Sp1 and Egr1, in the regulation of hsa-miR-106a expression and concomitant decrease in the IL-10 expression, has also been demonstrated. In summary, our results showed that IL-10 expression may be regulated by miR-106a, which is in turn transcriptionally regulated by Egr1 and Sp1.Egr1 ͉ IL-10 ͉ microRNA ͉ SP1 ͉ miR-106a promoter

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Section: Rna Extraction and Rt-pcrmentioning

confidence: 99%

“…Nuclear protein was prepared as described previously (33), and Western blotting was performed for Egr1, Sp1, and Lamin, as described in SI Materials and Methods.…”

Section: Rna Extraction and Rt-pcrmentioning

confidence: 99%

Posttranscriptional regulation of interleukin-10 expression by hsa-miR-106a

Sharma

Kumar

Aich

et al. 2009

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

182

128

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“…These proteins were downregulated by inhibition of NF-jB via suppression of IKK, ERK1/2 and reduction in p38 phosphorylation. Inhibition of NF-jB activation by blocking the phosphorylation and subsequent degradation of IjBa has also been reported independently by Kumar et al and Kwon et al [81,84]. Isoliquiritigenin (2) potentiated HO-1 expression through the ERK1/2 pathway in RAW 267.4 cells, thus effectively inhibiting macrophage-derived inflammation [93].…”

Section: Inflammation Cell-signaling Pathways Targeted By Chalconesmentioning

confidence: 73%

Dietary chalcones with chemopreventive and chemotherapeutic potential

et al. 2011

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Chalcones are absorbed in the daily diet and appear to be promising cancer chemopreventive agents. Chalcones represent an important group of the polyphenolic family, which includes a large number of naturally occurring molecules. This family possesses an interesting spectrum of biological activities, including antioxidative, antibacterial, anti-inflammatory, anticancer, cytotoxic, and immunosuppressive potential. Compounds of this family have been shown to interfere with each step of carcinogenesis, including initiation, promotion and progression. Moreover, numerous compounds from the family of dietary chalcones appear to show activity against cancer cells, suggesting that these molecules or their derivatives may be considered as potential anticancer drugs. This review will focus primarily on prominent members of the chalcone family with an 1,3-diphenyl-2-propenon core structure. Specifically, the inhibitory effects of these compounds on the different steps of carcinogenesis that reveal interesting chemopreventive and chemotherapeutic potential will be discussed.

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“…In the present study, 15 μM Aβ (25-35) significantly increased the ROS level in cultured neurons, and this was inhibited by SSB. In addition, the constituents of SSB contain antioxidant components (Du and Ko, 2006;Ha et al, 2009a,b;Kumar et al, 2007). Therefore, it is possible that the favorable effect of SSB on Aβ (25-35)-induced cognitive deficits can be attributed to the inhibition of ROS generation.…”

Section: Discussionmentioning

confidence: 99%

“…These results indicate that SSB might prevent Aβ (25-35)-induced Ca 2+ entry through VDCC-and / or NMDA-receptor-coupled channels to inhibit ROS generation and then neuronal death, although the mechanism by which SSB blocks the channels is not clear. Vitis amurensis, Aralia cordata, and Glycyrrhizae radix, the constituents of SSB, have been reported to possess antioxidant principles, resveratrol, oleanolic acid, and isoliquirtigenin (Du and Ko, 2006;Ha et al, 2009a, b;Kumar et al, 2007), respectively, suggesting that inhibition of Aβ (25-35)-induced neuronal death by SSB might be due to their ROS scavenging activity. Further Many studies have demonstrated that Aβ is accompanied by multiple events culminating in apoptosis in in vitro neuronal experiments (Ekinci et al, 2000;Yan et al, 1999).…”

Section: Ssb Inhibited Aβ (25-35)-induced Memory Impairmentmentioning

confidence: 99%

Inhibitory Effect of an Ethanol Extract Mixture of Vitis amurensis, Aralia cordata, and Glycyrrhizae radix on Amyloid β Protein (25-35)-Induced Neurotoxicity

Jang

Seong²

2014

Korean Journal of Medicinal Crop Science

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: The present study investigated an ethanol extract (SSB) of a mixture of three medicinal plants of Vitis amurensis, Aralia cordata, and Glycyrrhizae radix for possible neuroprotective effects on neurotoxicity induced by Amyloid β protein (Aβ) (25)(26)(27)(28)(29)(30)(31)(32)(33)(34)(35) . From these results, it is suggested that antidementia effect of SSB is due to its neuroprotective effect against Aβ (25-35)-induced neurotoxicity and that SSB may have a therapeutic role in preventing the progression of Alzheimer's disease.

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Isoliquiritigenin inhibits IκB kinase activity and ROS generation to block TNF-α induced expression of cell adhesion molecules on human endothelial cells

Cited by 110 publications

References 35 publications

Posttranscriptional regulation of interleukin-10 expression by hsa-miR-106a

Posttranscriptional regulation of interleukin-10 expression by hsa-miR-106a

Dietary chalcones with chemopreventive and chemotherapeutic potential

Inhibitory Effect of an Ethanol Extract Mixture of Vitis amurensis, Aralia cordata, and Glycyrrhizae radix on Amyloid β Protein (25-35)-Induced Neurotoxicity

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