Isolierung von Streptothricin-resistenten Mutanten ausEscherichia coli K12, Stamm A19

Seidel, Laura; Haupt, Ina

doi:10.1002/jobm.3630220209

Z Allg Mikrobiol

1982

DOI: 10.1002/jobm.3630220209

|View full text |Cite

Isolierung von Streptothricin-resistenten Mutanten ausEscherichia coli K12, Stamm A19

Laura Seidel¹,

Ina Haupt²

Abstract: Mutants with various levels of resistance to streptothricin were isolated from Escherichia coli K12, strain A19 after mutagenesis with N-methyl-N-nitro-N-nitroso-guanidine and ethylmethane-sulfonate. Nourseothricin, a mixture of streptothricin F and D was the selection agent. Spontaneous resistant mutants could not be found. The streptothricin-resistant mutant E. coli A19 Stcr 2/2/1 shows cross-resistance to some of the aminoglycoside antibiotics investigated, but no cross-resistance to chloramphenicol and chl… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...

Citation Types

Supporting

Mentioning

Contrasting

Year Published

1984

2017

Publication Types

Select...

Article5

Relationship

Self Cite0

Independent5

Authors

Journals

Cited by 5 publications

(1 citation statement)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The presence of streptothricin results in the mistranslation of mRNAs (10) and its inhibitory effect increases as a function of the length of the ␤-lysine chain (11). Streptothricin inhibits protein synthesis by preventing binding of charged tRNAs to the A-site of the ribosome and blocking translocation of the peptidyl-tRNA to the P-site (12,13). Streptothricin resistance can be caused by structural modification of the antibiotic.…”

mentioning

confidence: 99%

In Bacillus subtilis, the SatA (Formerly YyaR) Acetyltransferase Detoxifies Streptothricin via Lysine Acetylation

Burckhardt

Escalante‐Semerena

2017

Appl Environ Microbiol

View full text Add to dashboard Cite

Soil is a complex niche, where survival of microorganisms is at risk due to the presence of antimicrobial agents. Many microbes chemically modify cytotoxic compounds to block their deleterious effects. Streptothricin is a broad-spectrum antibiotic produced by streptomycetes that affects Gram-positive and Gram-negative bacteria alike. Here we identify the SatA (for treptothricinceyltransferase , formerly YyaR) enzyme of as the mechanism used by this soil bacterium to detoxify streptothricin. strains lacking were susceptible to streptothricin. Ectopic expression of restored streptothricin resistance to (SatA) strains. Purified SatA acetylated streptothricin at the expense of acetyl-coenzyme A (acetyl-CoA). A single acetyl moiety transferred onto streptothricin by SatA blocked the toxic effects of the antibiotic. SatA bound streptothricin with high affinity ( [dissociation constant] = 1 μM), and did not bind acetyl-CoA in the absence of streptothricin. Expression of in conferred streptothricin resistance, indicating that SatA was necessary and sufficient to detoxify streptothricin. Using this heterologous system, we showed that the SatA homologue from also had streptothricin acetyltransferase activity. Our data highlight the physiological relevance of lysine acetylation for the survival of in the soil. Experimental support is provided for the functional assignment of gene products of the soil-dwelling bacilli and This study focuses on one enzyme that is necessary and sufficient to block the cytotoxic effects of a common soil antibiotic. The enzyme alluded to is a member of a family of proteins that are broadly distributed in all domains of life but poorly studied in and The initial characterization of the enzyme provides insights into its mechanism of catalysis.

show abstract

mentioning

confidence: 99%

In Bacillus subtilis, the SatA (Formerly YyaR) Acetyltransferase Detoxifies Streptothricin via Lysine Acetylation

Burckhardt

Escalante‐Semerena

2017

Appl Environ Microbiol

View full text Add to dashboard Cite

show abstract

Bacterial resistance to streptothricins

Haupt¹,

Thrum²

1985

J. Basic Microbiol.

View full text Add to dashboard Cite

Resistance to streptothricin was studied in bacteria with different resistance mechanisms. The laboratory-induced streptothricin-resistant mutant E. coli A19 Stcr 2/2/1 showed a high level of cross-resistance to aminoglycosides and other miscoding inducing antibiotics. In contrast, aminoglycosid-resistant E. coli strains with plasmid-determined aminoglycoside modifying enzymes were sensitive to streptothricin. Enzymatic inactivation of streptothricin by acetylation was demonstrated for the streptothricin producing Streptomyces noursei, strain NG13. This strain showed no cross-resistance to miscoding inducing aminoglycosides.

show abstract

Spread of plasmid‐mediated nourseothricin resistance due to antibiotic use in animal husbandry

Hummel

Tschäpe

Witte

1986

J. Basic Microbiol.

108

View full text Add to dashboard Cite

After using of the streptothricin antibiotic nourseothricin in animal husbandry for growth promotion, plasmid-borne resistance to streptothricin could be observed in E. coli from nourseothricin fed pigs, from employees in pig farms and from their family members. Moreover, streptothricin resistance plasmids also occurred in E. coli of man without any contact to pig farms (gut flora and even urinary tract infections). However, these individuals live in villages and towns of the territory where nourseothricin was applied to pigs. Similar streptothricin resistance plasmids belonging to different incompatibility groups were found in both E. coli from pigs and E. coli from human beings. As no coselection of resistance to drugs indispensable for therapeutic use in man was observed, the application of nourseothricin in animal husbandry has not clinical implication for human medicine yet. Nevertheless, this problem remains under further investigation.

show abstract

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

hi@scite.ai

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

Isolierung von Streptothricin-resistenten Mutanten ausEscherichia coli K12, Stamm A19

Cited by 5 publications

References 12 publications

In Bacillus subtilis, the SatA (Formerly YyaR) Acetyltransferase Detoxifies Streptothricin via Lysine Acetylation

In Bacillus subtilis, the SatA (Formerly YyaR) Acetyltransferase Detoxifies Streptothricin via Lysine Acetylation

Bacterial resistance to streptothricins

Spread of plasmid‐mediated nourseothricin resistance due to antibiotic use in animal husbandry

Contact Info

Product

Resources

About