Isolierung und Identifizierung von Pasteurellaceae (Haemophilus, Pasteurella, Actinobacillus und verwandte Taxa)

Mannheim, W.

doi:10.1016/s0934-8840(89)80095-7

Cited by 13 publications

(9 citation statements)

References 33 publications

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“…However, factors such as type of test, nature and composition of media, pH range of indicators and time of incubation are frequent variables that may give rise to different results between laboratories. To provide an example of a set of standard conditions used for phenotypic characterization, we have adopted the protocols used in two Danish and one German laboratory for decades (Mannheim, 1989;M. Bisgaard and W. C. Frederiksen, unpublished), resulting in highly reproducible and comparable results (see Angen et al, 1997a).…”

Section: Methodsmentioning

confidence: 99%

“…The methods described in Table 1 have mainly been adopted from Bisgaard et al (1991) with reference to Barrow & Feltham (1993). Consultation of the major publications dealing with phenotypic characterization of members of the Pasteurellaceae is further recommended (Kilian, 1976;Kilian & Frederiksen, 1981;Sneath & Stevens, 1985;Mannheim, 1989). The incubation temperature is 37 u C for all isolates unless otherwise stated.…”

Section: Methodsmentioning

confidence: 99%

“…The test can be performed on blood agar base with 5 % calf blood by inoculating a blood agar plate with the test organism at a right angle to the inoculated line of a 'feeder' strain of an Acinetobacter species, Staphylococcus epidermidis or Staphylococcus aureus. The Vfactor supplied by the feeder strain will give rise to 'satellitism' of the tested isolate along the V-factor-producing isolate (Mannheim, 1989). It is important to support the V-factor requirement of such strains in further tests.…”

Section: Methodsmentioning

confidence: 99%

“…In the case of X-and/or V-factor requirements, these substrates can be added at appropriate concentrations as sterile solutions (Mannheim, 1989;Barrow & Feltham, 1993).…”

Section: Methodsmentioning

confidence: 99%

“…Common physiological and biochemical traits for members of the 12 named genera of the Pasteurellaceae Further details of the tests are available in the appendix. Data were taken from Mannheim (1989) and Christensen (2005).…”

Section: Taxonomic Investigation At the Genus Levelmentioning

confidence: 99%

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Proposed minimal standards for the description of genera, species and subspecies of the Pasteurellaceae

Christensen

Kuhnert

Busse

et al. 2007

International Journal of Systematic and Evolutionary Microbiology

114

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4Rødding Tvaervej 3, 6630 Rødding, DenmarkPrinciples and guidelines are presented to ensure a solid scientific standard of papers dealing with the taxonomy of taxa of Pasteurellaceae Pohl 1981. The classification of the Pasteurellaceae is in principle based on a polyphasic approach. DNA sequencing of certain genes is very important for defining the borders of a taxon. However, the characteristics that are common to all members of the taxon and which might be helpful for separating it from related taxa must also be identified. Descriptions have to be based on as many strains as possible (inclusion of at least five strains is highly desirable), representing different sources with respect to geography and ecology, to allow proper characterization both phenotypically and genotypically, to establish the extent of diversity of the cluster to be named. A genus must be monophyletic based on 16S rRNA gene sequence-based phylogenetic analysis. Only in very rare cases is it acceptable that monophyly can not be achieved by 16S rRNA gene sequence comparison. Recently, the monophyly of genera has been confirmed by sequence comparison of housekeeping genes. In principle, a new genus should be recognized by a distinct phenotype, and characters that separate the new genus from its neighbours should be given clearly. Due to the overall importance of accurate classification of species, at least two genotypic methods are needed to show coherence and for separation at the species level. The main criterion for the classification of a novel species is that it forms a monophyletic group based on 16S rRNA gene sequence-based phylogenetic analysis. However, some groups might also include closely related species. In these cases, more sensitive tools for genetic recognition of species should be applied, such as DNA-DNA hybridizations. The comparison of housekeeping gene sequences has recently been used for genotypic definition of species. In order to separate species, phenotypic characters must also be identified to recognize them, and at least two phenotypic differences from existing species should be identified if possible. We recommend the use of the subspecies category only for subgroups associated with disease or similar biological characteristics. At the subspecies level, the genotypic groups must always be nested within the boundaries of an existing species. Phenotypic cohesion must be documented at the subspecies level and separation between subspecies and related species must be fully documented, as well as association with particular disease and host. An overview of methods previously used to characterize isolates of the Pasteurellaceae has been given. Genotypic and phenotypic methods are separated in relation to tests for investigating diversity and cohesion and to separate taxa at the level of genus as well as species and subspecies. et al., 1992). However, it makes no sense to name a novel taxon without having considered its specific properties and evaluated how it differs from related taxa. To ensure the optimal circumscript...

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…In the case of X-and/or V-factor requirements, these substrates can be added at appropriate concentrations as sterile solutions (Mannheim, 1989;Barrow & Feltham, 1993).…”

Section: Methodsmentioning

confidence: 99%

Section: Taxonomic Investigation At the Genus Levelmentioning

confidence: 99%

See 3 more Smart Citations