Isolation, structure elucidation and anticancer activity from Brevibacillus brevis EGS 9 that combats Multi Drug Resistant actinobacteria

Arumugam, T.; Kumar, P. Senthil; Hemavathy, R.V.; Swetha, V.; Sri, R. Karishma

doi:10.1016/j.micpath.2017.12.061

Cited by 7 publications

(5 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For example, brevilaterin B and other peptides extracted from Brevibacillus laterosporus S62-9 were recognized for their anticancer, antibacterial, and antifungal activities [13]. Similar effects were generated by Brevibacillus brevis EGS9 strain, as reported previously [14]. Moreover, bogorol, a peptide isolated from Brevibacillus laterosporus JX-5, exhibited potent antibacterial and anticancer activities [15].…”

Section: Introductionsupporting

confidence: 73%

Structural Diversity, LC-MS-MS Analysis and Potential Biological Activities of Brevibacillus laterosporus Extract

et al. 2022

View full text Add to dashboard Cite

Lake Mariout is Egypt’s degraded coastal marine habitat that encompasses a variety of wastes. The biodiversity and hard environmental conditions allow the co-existence of organisms with high resistance and rich metabolism, making them potential candidates for screening and isolating novel microbial strains. A bacterial isolate (BF202) cultured from the marine sediments of Alexandria’s Mariout Lake (Egypt) was tested for its antimicrobial and anticancer potential. The phylogenetic analysis of the isolated strain’s 16S rDNA and gyrB revealed that BF202 belongs to Brevibacillus laterosporus (B. laterosporus). Antibiosis of B. laterosporus was confirmed against microbial pathogens including Escherichia coli, Klebsiella pneumoniae, Salmonella typhi, and Staphylococcus aureus. The highest antibacterial activity was detected on glucose peptone medium after 18 h of incubation at 35 °C, and at pH of 7.0 in the presence of mannose and ammonium carbonate as carbon and nitrogen sources, respectively. The cytotoxicity of the methanolic extract against breast cancer (MCF-7) and normal Vero cell lines, using the MTT test, revealed IC50 values of 7.93 and 23.79 µg/mL, respectively. To identify apoptotic and necrotic cells, a flow cytometric analysis using annexin V-FITC/PI dual-labeling was utilized and recorded a higher number of necrotic cells compared to apoptotic ones. Similarly, the cell cycle S-phase arrest was reported. The LC-MS-MS investigation of B. laterosporus extract and the molecular networking database analysis demonstrated five strategic diketopiperazine compounds with antimicrobial and anticancer activities. Taken together, this research shows that the crude extract of B. laterosporus might be an effective agent against drug-resistant bacteria and malignant disorders due to its richness in diketopiperazines.

show abstract

Section: Introductionsupporting

confidence: 73%

Structural Diversity, LC-MS-MS Analysis and Potential Biological Activities of Brevibacillus laterosporus Extract

et al. 2022

View full text Add to dashboard Cite

show abstract

“…and Bacillus sp. (ND) Algicidal [24,37] Bacillus subtilis and Achromobacter kerstersii (sea and rizhosphere soil) Antifungal [30,47] Streptomyces (mangrove soil) Antioxidant [48,49] -dione, hexahydro,3-(2-methyl propyl) Staphylococcus sp. (Deep Sea sediments and marine sponges)…”

Section: Pyrrolo-pyrazinesmentioning

confidence: 99%

“…Antimicrobial and anticancer [38,50] Brevibacillus brevis (ND) Anticancer [37] Pseudomonas putida (ND) ND [51] Bacillus sp. and Achromobacter kerstersii (ND) Antifungal [15,30] Nocardiopsis sp.…”

Section: Pyrrolo-pyrazinesmentioning

confidence: 99%

“…Moreover, an increase in the catalase activity, leading to a decrease in intracellular ROS was also observed. GC/MS (Gas Chromatography coupled with Mass Spectrometry) analysis showed the presence of potential bioactive compounds such as pyrrolo[1, 2α]-pyrazine-1,4-dione hexahydro-, [37] pyrrolo[1,2-a]-pyrazine1,4-dione hexahydro-3-(2-methylpropyl) in the extract. [38,39] Most of these compounds are known for their antioxidant effect, but for the first time in this study, these compounds were related to anti-aging activity.…”

Section: Pseudomonas Sp (Nd)mentioning

confidence: 99%

See 1 more Smart Citation

Volatile Nitrogenous Compounds from Bacteria: Source of Novel Bioactive Compounds

Valença

Barbosa

Souto

et al. 2021

Chemistry & Biodiversity

View full text Add to dashboard Cite

Bacteria can produce nitrogenous compounds via both primary and secondary metabolic processes. Many bacterial volatile nitrogenous compounds produced during the secondary metabolism have been identified and reported for their antioxidant, antibacterial, antifungal, algicidal and antitumor activities. The production of these nitrogenous compounds depends on several factors, including the composition of culture media, growth conditions, and even the organic solvent used for their extraction, thus requiring their identification in specific conditions. In this review, we describe the volatile nitrogenous compounds produced by bacteria especially focusing on their antimicrobial activity. We concentrate on azo-compounds mainly pyrazines and pyrrolopyridines reported for their activity against several microorganisms. Whenever significant, extraction and identification methods of these compounds are also mentioned and discussed. To the best of our knowledge, this is first review describing volatile nitrogenous compounds from bacteria focusing on their biological activity.

show abstract

“…In previous studies, Brevibacillus was shown to be a widely effective biocontrol bacterium. It also has an inhibitory effect on many resistant fungi ( Arumugam et al, 2018 ) and can also control some hymenopterans ( Babar et al, 2022 ). B. brevis , as a biocontrol strain, has great research potential in different fields.…”

Section: Introductionmentioning

confidence: 99%

Mechanism of Brevibacillus brevis strain TR-4 against leaf disease of Photinia×fraseri Dress

Ji,

Li,

Yao

et al. 2024

PeerJ

View full text Add to dashboard Cite

Background Colletotrichum species are among the most common pathogens in agriculture and forestry, and their control is urgently needed. Methods In this study, a total of 68 strains of biocontrol bacteria were isolated and identified from Photinia × fraseri rhizosphere soil. Results The isolates were identified as Brevibacillus brevis by 16S rRNA. The inhibitory effect of TR-4 on Colletotrichum was confirmed by an in vitro antagonistic experiment. The inhibitory effect of TR-4 was 98% at a concentration of 10 µl/ml bacterial solution, protection of the plant and inhibition of C. siamense was evident. Moreover, the secretion of cellulase and chitosan enzymes in the TR-4 fermentation liquid cultured for three days was 9.07 mol/L and 2.15 µl/mol, respectively. Scanning electron microscopy and transmission electron microscopy confirmed that TR-4 destroyed the cell wall of C. siamense, resulting in leakage of the cell contents, thus weakening the pathogenicity of the bacteria.

show abstract

Isolation, structure elucidation and anticancer activity from Brevibacillus brevis EGS 9 that combats Multi Drug Resistant actinobacteria

Cited by 7 publications

References 19 publications

Structural Diversity, LC-MS-MS Analysis and Potential Biological Activities of Brevibacillus laterosporus Extract

Structural Diversity, LC-MS-MS Analysis and Potential Biological Activities of Brevibacillus laterosporus Extract

Volatile Nitrogenous Compounds from Bacteria: Source of Novel Bioactive Compounds

Mechanism of Brevibacillus brevis strain TR-4 against leaf disease of Photinia×fraseri Dress

Contact Info

Product

Resources

About