Isolation strategies of marine-derived actinomycetes from sponge and sediment samples

Hameş-Kocabaş, E. Esin; Uzel, Ataç

doi:10.1016/j.mimet.2012.01.010

Cited by 46 publications

(35 citation statements)

References 51 publications

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“…Thus, antibiotics and their production and resistance loci detectable in multi-omics data can be used as selection criteria for the isolation of target organisms. This may include the prevention of overgrowth by fast-growing microorganisms (Sizova et al 2012;Hames-Kocabas & Uzel 2012;Rettedal et al 2014;Keren et al 2015) or selection for specific phenotypic traits such as those characteristic for Gram-positive bacteria or production of antibiotic-resistant spores.…”

Section: Cooperative and Antagonistic Interactions Within Microbial Cmentioning

confidence: 99%

The multi-omics promise in context: from sequence to microbial isolate

Gutleben

Mares

Elsas

et al. 2017

Critical Reviews in Microbiology

163

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The numbers and diversity of microbes in ecosystems within and around us is unmatched, yet most of these microorganisms remain recalcitrant to in vitro cultivation. Various high-throughput molecular techniques, collectively termed multi-omics, provide insights into the genomic structure and metabolic potential as well as activity of complex microbial communities. Nonetheless, pure or defined cultures are needed to (1) decipher microbial physiology and thus test multiomics-based ecological hypotheses, (2) curate and improve database annotations and (3) realize novel applications in biotechnology. Cultivation thus provides context. In turn, we here argue that multi-omics information awaits integration into the development of novel cultivation strategies. This can build the foundation for a new era of omics information-guided microbial cultivation technology and reduce the inherent trial-and-error search space. This review discusses how information that can be extracted from multi-omics data can be applied for the cultivation of hitherto uncultured microorganisms. Furthermore, we summarize groundbreaking studies that successfully translated information derived from multi-omics into specific media formulations, screening techniques and selective enrichments in order to obtain novel targeted microbial isolates. By integrating these examples, we conclude with a proposed workflow to facilitate future omics-aided cultivation strategies that are inspired by the microbial complexity of the environment. ARTICLE HISTORY

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Section: Cooperative and Antagonistic Interactions Within Microbial Cmentioning

confidence: 99%

The multi-omics promise in context: from sequence to microbial isolate

Gutleben

Mares

Elsas

et al. 2017

Critical Reviews in Microbiology

163

View full text Add to dashboard Cite

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“…The samples collected were either frozen or stored at 4°C and were processed under sterile conditions on the same day or the next. Five sediment samples and a clump of the green-grey sponge Halichondria panicea (Hooper & van Soest, 2002;Little, 1963) The sediment samples were processed using previously reported methods (Gontang, Fenical, & Jensen, 2007;Hameş-Kocabaş & Uzel, 2012;Öner et al, 2014) in order to facilitate the growth of spore forming Gram-positive bacteria which are associated with the production of bioactive chemical entities. They were processed using one of four methods: Method 1 (wet/stamp), under sterile conditions, 90% of the sea water was poured off, and a sterile cotton swab used to lightly stamp the sediments onto selected agar media; Method 2 (dry/dilute), a small spatula of sediment (c 0.5 g) was transferred to a sterile glass Petri dish, allowed to dry overnight, and then diluted with sterile sea water (5 ml).…”

Section: Sample Collection and Processingmentioning

confidence: 99%

Identification and bioactive potential of marine microorganisms from selected Florida coastal areas

Christensen

Martin

2017

MicrobiologyOpen

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The ocean, with its rich untapped chemical biodiversity, continues to serve as a source of potentially new therapeutic agents. The evaluation of the diversity of cultivable microorganisms from the marine sponge Halichondria panicea and ocean sediment samples were examined and their potential as sources of antimicrobial and antiproliferative agents were investigated. The marine sponge and sediments were collected at different depths (0.9–6 meters) and locations in Florida, including Florida Keys, Port St. Joe in Pensacola, Pensacola Bay, Pensacola Beach, and Fort Pickens. Twenty‐one cultivatable isolates were grouped according to their morphology and identified using 16S rRNA molecular taxonomy. The bacterial community identified consisted of members belonging to the Actinobacteria, Bacteroidetes, Proteobacteria (Alpha‐ and Gamma‐classes) and Firmicutes phylogeny. Seven of the microbes exhibited mild to significant cytotoxic activities against five microbial indicators but no significant cytotoxic activities were observed against the pancreatic (PANC‐1) nor the multidrug‐resistant ovarian cancer cell lines (NCI/ADR). This work reaffirms the phyla Actinobacteria and Proteobacteria as sources of potential bioactive natural product candidates for drug discovery and development.

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“…The peptide-polyketide glycoside totopotensamide A (42) and its aglycone totopotensamide B (43), new natural products isolated from the molluscassociated Streptomyces sp. [87].…”

Section: Polyketidesmentioning

confidence: 99%

“…Marine actinobacteria are the potential resource for natural products [39][40][41][42][43]. Many of the natural products derived from the marine actinobacteria possess unique structural features and molecular modes, which lead to drug development [44].…”

Section: Introductionmentioning

confidence: 99%