Isolation, Serotyping, Pathogenicity and Antibiotic Sensitivity Testing of Escherichia Coli from Broiler Chickens in Egypt

Ellakany, Hany F.; Abdel-Hamid, Hatem S.; Ibrahim, Madiha S.; Mostafa, Nagwa S.; Elbestawy, Ahmed R.; Gado, Ahmed R.

doi:10.5455/ajvs.10916

Cited by 4 publications

(3 citation statements)

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“…Here, E. coli strains have been detected in 17 out of 100 samples with isolation rate of 17%. Previous Egyptian studies on E. coli revealed either higher isolation rates 75% (Abd El-Tawab et al 2015a), 43.1% (Heba et al 2012), 35% (Amer et al 2018, 34.6% (Ellakany et al 2019) and27.7% (Abd El-Mongy et al 2017), while other showed lower isolation rate 8.3% (Ahmed et al 2017). These differences in the isolation rates may be owing to the differences in the number of samples, the age of birds, the geographic regions, the isolation methods, and previous medication.…”

Section: Discussionmentioning

confidence: 91%

“…Avian colibacillosis caused by Escherichia coli (E. coli) is one of the main widespread bacterial infection that causes losses and a decrease in the production (Ronco et al 2017;Ellakany et al 2019;Mehmood et al 2020). Some strains of E. coli are presented as intestinal commensal of birds, but in weak immune hosts, the pathogenic or even non-pathogenic strains can cause diseases conditions (Sarba et al 2019).…”

Section: Introductionmentioning

confidence: 99%

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Isolation, Characterization and Pathogenicity of the Most Common Bacteria Associated with Gut Health in Egyptian Broiler Chicken Flocks

2022

Int J Vet Sci

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This study was designed to isolate and characterize the pathogenicity of the most common bacteria causing enteric diseases in broilers in some Egyptian governorates. Enteric bacterial organisms like Escherichia coli (E. coli), Salmonellae, and Clostridia perfringens (C. perfringens) were isolated and identified from 100 dead and diseased broilers in Giza, El-Kalubia and El-Sharqia governorates. The samples were subjected to conventional isolation as well as biochemical and serological identification techniques. Molecular and toxigenic detection of C. perfringens were performed on one selected isolate by multiplex PCR. The pathogenicity test of some isolated bacterial strains was done on 80 a-day-old broiler chicks. The clinical observation, performance parameters, bacterial re-isolation and histopathological examination were carried out after bacterial challenge. The results revealed isolation of E. coli, Salmonella and C. perfringens in rates of 17, 11 and 39%, respectively. Serological identification of E. coli revealed that O78 (35.2%) and O1 (23.5%) were the highest isolated serotypes, while O117 (17.6%), O91 (11.7%), O112, and O146 (5.8% each) were the lowest ones. Salmonella enteritidis (S. enteritidis) was the most prevalent (36.3%) followed by S. kentucky, S. larochelle (27.2%, each) and S. inganda (9%). Multiplex PCR of C. perfringens strains revealed presence of both cpa and cpb genes that encoding to alpha (α) and beta (β) lethal toxins, respectively. The pathogenicity of E. coli O78, S. enteritidis, and C. perfringens were tested in broiler chickens. The results showed that E. coli O78, S. enteritidis and C. perfringens were pathogenic strains. It was concluded that enteric bacterial pathogens especially E. coli O78, S. enteritidis, and C. perfringens type C are widely distributed in Egyptian broiler chicken flocks and still causing severe losses because of mortalities and decreasing in production.

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Section: Discussionmentioning

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Isolation, Characterization and Pathogenicity of the Most Common Bacteria Associated with Gut Health in Egyptian Broiler Chicken Flocks

2022

Int J Vet Sci

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“…From our data the most predominant serotypes were O91, O128, O78, O124, O2 and O44 and these strains were related to EHEC, EPEC, ETEC, and EIEC. In old studies, most avian pathogenic E. coli strains linked to colibacillosis outbreaks were O1, O2, O15, O35, and O78 serotypes [41], but recently new serotypes have been emerged as APEC [42] and other authors reported different serotypes in Egypt as O78, O1, O2, O91, and O8 by Younis et al [43] from Mansoura governorates, serotypes O78, O24, O44, O55, O86, O124, O158 and O127 by Amer et al [44] from Giza and Kaluobia governorates and serotypes O169, O115, and O29 by Ellakany et al [45] from Alexandria governorates.…”

Section: Discussionmentioning

confidence: 99%

Molecular Identification of Resistance and Pathogenicity Genes of E. coli Isolated From Broiler Chicken Farms

Ahmed,

Salem,

Hamoud

et al. 2024

Egyptian Journal of Veterinary Sciences

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scherichia coli (E. coli) is a widespread avian pathogen and usually is considered as a secondary pathogen for different infectious agents causing huge financial losses in the poultry industry. Thus, the current works aim to molecular detection of resistance and pathogenicity genes of E. coli isolated from broiler chicken farms. A total of 18 flocks out of 30 broiler flocks (60%) were positive for E. coli infection. On the base of the Congo red (CR) binding assay, 15 flocks out of 18 were identified as pathogenic E. coli (83.3%) and 3 flocks appeared as nonpathogenic E. coli (16.6%). All pathogenic E. coli were subjected to in vitro antibiotic sensitivity testing to select the most resistant isolates. Ten pathogenic E. coli isolates representing ten different broiler flocks were subjected for molecular identification via polymerase chain reaction (PCR) for both resistance and virulence genes. The most predominant isolated serotypes were O91, O128, O78, O124, O 2 and O44. These strains were related to EHEC, EPEC, ETEC, and EIEC. These E. coli isolates are multidrug resistant (MDR) to extensively drug-resistant (XDR). The virulence genes tsh, papC, iss, iutA, and hlyF were detected in 50% of isolates having 5 genes and 50% having 4 genes. All the tested isolates showed MDR genes 60% of isolates were positive for 5 resistance genes and 20% were positive for 4 resistance genes and 20% were positive for resistance 3 genes. We can conclude that E. coli continues to threaten poultry industry and further studies are recommended to found safe antibiotic natural alternatives to overcome E. coli MDR existing strains side by side with strengthening the bird's immunity and application of strict hygienic measures.

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Comparative evaluation of a live E. coli vaccine and cefotaxime treatment against three E. coli serotypes in broilers

Elbestawy

Ellakany²,

El-Hamid³

et al. 2021

Journal of King Saud University - Science

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Isolation, Serotyping, Pathogenicity and Antibiotic Sensitivity Testing of Escherichia Coli from Broiler Chickens in Egypt

Cited by 4 publications

References 0 publications

Isolation, Characterization and Pathogenicity of the Most Common Bacteria Associated with Gut Health in Egyptian Broiler Chicken Flocks

Isolation, Characterization and Pathogenicity of the Most Common Bacteria Associated with Gut Health in Egyptian Broiler Chicken Flocks

Molecular Identification of Resistance and Pathogenicity Genes of E. coli Isolated From Broiler Chicken Farms

Comparative evaluation of a live E. coli vaccine and cefotaxime treatment against three E. coli serotypes in broilers

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