Isolation, purification and properties of lipase from Pseudomonas aeruginosa

Zouaoui, Benattouche

doi:10.5897/ajb11.3951

Cited by 3 publications

(4 citation statements)

References 22 publications

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“…For determining the effect of metal ions on lipase activity, the purified enzyme was pre incubated with 1 mM of Ca 2+ , Mg 2+ , Cu 2+ , Fe 2+ and Zn 2+ for 1 h at 37°C and the residual activity was determined by using olive oil as substrate, according to Zouaoui et al, [24].…”

Section: Effect Of Metal Ions On Lipase Activitymentioning

confidence: 99%

Isolation, Purification, and production of lipase from Bacillus subtilis isolated from food processing wastes and its application in biodiesel production

et al. 2023

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Massive amounts of waste oil generated by restaurants and native homes in Egypt are dumped into sewage systems, causing network damage. Utilization of waste cooking oil (WCO) in biodiesel production, can aid in solving the problem. In the present study, a new lipase-producing bacterium (Bacillus subtilis) was isolated, identified using 16S rRNA sequence analysis and the isolate sequence was deposited in GenBank (accession number: MN238705). The lipase coding sequence was amplified from the abovementioned strain cloned, and expressed in Escherichia coli. The molecular weight of the purified enzyme tagged with glutathione-S-transferase (GST) was approximately 49 KDa on SDS-PAGE. Accordingly, the purified native lipase exhibited maximal hydrolytic activity at 37°C and pH 7.0, with a positive effect for Mg 2+ and Ca 2+ metals on its activity. Finally, purified native lipase was immobilized on Amberlite resin (IRC50) and successfully catalyzed the transformation of WCO into biodiesel with a yield of 87.39% as determined by gas chromatography/mass spectrometry (GC/MS) analysis. The physicochemical properties of generated biodiesel have met European standards. Prospective studies include large-scale production of Bacillus subtilis native lipase and testing the efficacy of recombinant lipase for the transformation of WCO into biodiesel.

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Section: Effect Of Metal Ions On Lipase Activitymentioning

confidence: 99%

Isolation, Purification, and production of lipase from Bacillus subtilis isolated from food processing wastes and its application in biodiesel production

et al. 2023

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“…This technique is more simple and reliable when compared to the ones previously discussed. Olive oil has also been reported as a suitable substrate for screening of lipolytic microorganisms [41].…”

Section: Screening and Isolation Of Lipase-producing Microbesmentioning

confidence: 99%

“…A more reliable but laborious method is the titrimetric assay for lipases [42]. The use of this method has been effectively reported in the measurement of true lipase by [36,41].…”

Section: Lipase Activity Assaymentioning

confidence: 99%

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Microbial Lipases: A Prospect for Biotechnological Industrial Catalysis for Green Products: A Review

Ugo¹,

Amara²,

Cn³

et al. 2017

Ferment Technol

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Microbes have been novel hosts for suitable industrial enzymes including lipases. With the rapid increase in biotechnological industries, there is need for improvement of bioproducts, enhance environmental safety and product yield. Microbes are qualified biological arsenal for the achievement of the above mentioned targets in the industrial sectors. Lipases as versatile biological catalyst has given a promising prospect in meeting the needs for most industries such as biodiesel, foods and drinks, leather, textile, detergents, pharmaceuticals and medicals. Catalytic importance of lipases includes hydrolysis, esterification and transesterification. Each of the mentioned reactions has their industrial applications. Lipases exhibit various properties, with respect to its source. Immobilization has made the use of microbial lipases attain its optimum performance and hence suitable for various reaction and the need to add flavor to the immobilization processes.

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Biodegradation of waste greases and biochemical properties of a novel lipase from Pseudomonas synxantha PS1

et al. 2016

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A lipase-producing bacterial strain was isolated from oil-well-produced water in Shengli oilfield (Shandong province, China) and was identified as Pseudomonas synxantha by 16S rDNA sequence analysis (named Pseudomonas synxantha PS1). Strain PS1 showed a maximum lipase activity of 10.8 U/mL after culturing for 48 h at 30 °C, with lactose (4 g/L) as carbon source, tryptone (8 g/L) as nitrogen source, olive oil (0.5%, v/v) as inductor, and the initial pH 8.0. Meanwhile, the lipase gene from P. synxantha PS1 was cloned and expressed in Escherichia coli BL21 with the vector pET28a. The novel gene (lipPS1) has an open reading frame of 1425 bp and encodes a 474 aa lipase (LipPS1) sharing the most identity (87%) with the lipase in Pseudomonas fluorescens. LipPS1 preferably acted on substrates with a long chain (C10-C18) of fatty acids. The optimum pH and temperature of the recombinant enzyme were 8.0 and 40 °C, respectively, towards the optimum substrate p-nitrophenyl palmitate. The LipPS1 showed remarkable stability under alkaline conditions and was stable at pH 7.0-10.0 (retaining more than 60% activity). From the organic solvents tests, the lipase was activated by 15% (v/v) methanol (112%), 15% ethanol (127%), and 15% n-butyl alcohol (116%). LipPS1 presented strong biodegradability of waste grease; 93% of waste grease was hydrolyzed into fatty acid after 12 h at 30 °C. This is the first report of the lipase activity and lipase gene obtained from P. synxantha (including wild strain and recombinant strain) and of the recombinant LipPS1 with the detailed enzymatic properties. Also a preliminary study of the biodegradability of waste greases shows the potential value in industry applications.

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Isolation, purification and properties of lipase from Pseudomonas aeruginosa

Cited by 3 publications

References 22 publications

Isolation, Purification, and production of lipase from Bacillus subtilis isolated from food processing wastes and its application in biodiesel production

Isolation, Purification, and production of lipase from Bacillus subtilis isolated from food processing wastes and its application in biodiesel production

Microbial Lipases: A Prospect for Biotechnological Industrial Catalysis for Green Products: A Review

Biodegradation of waste greases and biochemical properties of a novel lipase from Pseudomonas synxantha PS1

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