Isolation, purification, and physicochemical characterization of a d-galactose-binding lectin from seeds of Erythrina speciosa

Konozy, Emadeldin Hassan E.; Bernardes, Emerson Soares; Rosa, Costa; Faça, Vitor M.; Greene, Lewis J.; Ward, Richard J.

doi:10.1016/s0003-9861(02)00695-1

Cited by 62 publications

(57 citation statements)

References 20 publications

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“…The R. prolixus trypanolytic factor's biological activity remained stable at room temperature during parasite counting (10 h) or at 7 to 10.5 pH, possibly due to the protein's chemical or physical stability not being affected, thereby conserving its native state. Lytic activity against T. rangeli KP1(-) continued in EGTA and EDTA chelate agent-treated hemolymph; on the other hand, we deduced that the trypanolytic factor did not belong to the metalloprotein family or any lectin requiring divalent cations for carrying out its normal biological activity as described by Ashida et al (1990, Yang et al (2000), Konozy et al (2003), Liu et al (2006).…”

Section: Discussionmentioning

confidence: 82%

Preliminary characterization of a Rhodnius prolixus hemolymph trypanolytic protein, this being a determinant of Trypanosoma rangeli KP1(+) and KP1(-) subpopulations' vectorial ability

Pulido

Pérez

Vallejo

2008

Mem. Inst. Oswaldo Cruz

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Rhodnius prolixus is the main Trypanosoma rangeli vector in several Latin-American countries and is susceptible to infection with KP1(+) strains; however, it presents an invasion-resistant response to KP1(-) strains. The present work has identified a trypanolytic protein against T. rangeli KP1(-) in the R. prolixus hemolymph which was fractioned with ammonium sulfate (following dialysis). The results revealed a protein component which did not depend on divalent cations for its biological function whilst keeping its trypanolytic activity at temperatures ranging from -20ºC to 37ºC, at 7.0 to 10.5 pH. The protein was partially purified by gel filtration chromatography and ionic exchange chromatography. The major component presented a molecular weight of around 79 kDa and an isoelectric point between 4.9 and 6.3 and may be directly related to hemolymph trypanolytic activity against T. rangeli KP1(-) populations.Key words: Rhodnius prolixus -Trypanosoma rangeli KP1(+) -Trypanosoma rangeli KP1(-) -trypanolytic factorvectorial ability -insect immunity Trypanosoma rangeli is a hemoflagellate which is apparently apathogenous for humans, presenting pathogenicity for the invertebrate host. T. rangeli is of great interest due to its presence in mixed infection with Trypanosoma cruzi in vertebrates and invertebrate hosts, thereby interfering with a clear diagnosis of Chagas' disease and the epidemiology of these parasites in different Latin-American countries .T. rangeli vectors become infected by ingesting blood from wild or domestic reservoirs in natural conditions. The parasite develops in the digestive tract and then invades the hemolymph where it is recognized by the vector's immune system; however, some strains may escape host defense mechanisms, reaching the salivary glands where they become infective forms or metacyclic trypomastigotes (D'Alessandro-Bacigalupo & Gore-Saravia 1992). Salivary gland invasion is a necessary step for transmission, being mediated by one or several ligandreceptor interactions (Basseri et al. 2002).Previous work has revealed two phylogenetically different groups in T. rangeli called T. rangeli KP1(-) and T. rangeli KP1(+), these being mainly transmitted by the 16 Rhodnius species described so far (Vallejo et al. 2002(Vallejo et al. , 2007. Specificity between the vector and the parasite strain which it transmits has been pointed out; each Rhodnius species seems to select the T. rangeli subpopulation to which it is susceptible in nature so that it can then be transmitted to the vertebrate host via insect bite . Nevertheless, a close association has been observed between T. rangeli KP1(+) and vectors from the prolixus group (which includes Rhodnius prolixus and Rhodnius neglectus) and between T. rangeli KP1(-) and vectors from the pallescens group (including Rhodnius pallescens, Rhodnius colombiensis and Rhodnius ecuadoriensis) , Urrea et al. 2005. Recent parasite-vector interaction studies have shown a trypanolytic factor in R. prolixus hemolymph impeding the development of T. rangeli KP1(...

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Section: Discussionmentioning

confidence: 82%

Preliminary characterization of a Rhodnius prolixus hemolymph trypanolytic protein, this being a determinant of Trypanosoma rangeli KP1(+) and KP1(-) subpopulations' vectorial ability

Pulido

Pérez

Vallejo

2008

Mem. Inst. Oswaldo Cruz

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“…The three lectins exhibited a three amino acid (GPN) domain in common with the D-galactose-binding lectin from Maclura pomifera (Osage orange) (Young et al, 1989). The acidic pI value of ScLL was typical of many galactose-specific lectins, for example, those from Erythrina species (Bhattacharyya et al, 1986;Konozy et al, 2002Konozy et al, , 2003, Maclura pomifera, Sophora japonica (Hankins et al, 1988), and Luetzelburgia auriculata (Oliveira et al, 2002). Although plant latex is often cytotoxic, neither the crude aqueous extract nor the purified lectin from the latex of S. carinatum showed any cytotoxic activity against J774 cells or murine peritoneal macrophages.…”

Section: Discussionmentioning

confidence: 99%

“…They are widely distributed in nature, particularly in the plant kingdom where they can be found in seeds, leaves, bark, bulbs, rhizomes, roots, cotyledons and tubers Witisuwannakul et al, 1998;Yagi et al, 2002;Oliveira et al, 2002;Konozy et al, 2002Konozy et al, , 2003. Seeds of leguminous species often contain large amounts of lectins that are similar to those present in other tissues of the same plant, including the latex.…”

Section: Introductionmentioning

confidence: 99%

Isolation and partial characterization of a D-galactose-binding lectin from the latex of Synadenium carinatum

Souza

Amâncio-Pereira

Cardoso

et al. 2005

Braz. arch. biol. technol.

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“…29 Contrariamente ao esperado, o dímero 6 não foi solúvel no meio, impossibilitando sua avaliação e comparação com 7, que apesar de também não ser muito solúvel, foi o suficiente para a avaliação. A potência inibitória de 7 no ensaio foi comparada à da lactose (4-O-(b-D-galactopiranosil)-D-glicose, dissacarídeo contendo um resíduo de D-galactose de configuração definida (configuração b), cuja potência foi considerada unitária e ao galactosídeo monomérico 9, preparado a partir de 4 por remoção dos grupos protetores seguida do acoplamento com etanolamina, utilizando-se N,N'-di-isopropilcarbodiimida (DIC) e HOBT como reagentes de acoplamento (Figura 2).…”

Section: Avaliação Da Interação Com a Lecunclassified

Síntese de β-D-galactopiranosídeos de arila diméricos para avaliação de sua interação com a lectina de Erythrina cristagalli

Figueiredo¹,

Meyer²,

Prado³

et al. 2009

Quím. Nova

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Recebido em 31/12/08; aceito em 13/4/09; publicado na web em 22/9/09 SYNTHESIS OF DIMERIC ARYL b-D-GALACTOPYRANOSIDES FOR THE EVALUATION OF THEIR INTERACTION WITH THE Erythrina cristagalli LECTIN.The synthesis of two new D-galactose-based dimers having a 1,4-butanediamine spacer is reported aiming at the evaluation of their interaction with the Erythrina cristagalli lectin. The title compounds were prepared in four and five steps from 2,3,4,6-tetra-O-acetyl-b-D-galactopyranoside bromide, in 20 % and 15 % overall yield, respectively, using the Doebner modification of the Koenavenagel reaction as the key sep. The lectin-carbohydrate interaction could be evaluated for only one dimer, due to solubility problems. A twofold enhancement of affinity was observed, compared to the corresponding monovalent ligand.Keywords: D-galactopyranose; glycodendrimers; Erythrina cristagalli. INTRODUÇÃOOs glicoconjugados como as glicoproteínas e os glicolipídios possuem um papel importante em vários processos biológicos relacionados com reconhecimento e adesão celular, como inflamação, infecção e mestástase.1,2 As forças que regem esses processos de reconhecimento e adesão são interações complexas entre as porções carboidrato de um glicoconjugado e as lectinas, proteínas que reconhecem especificamente carboidratos.Usualmente, a afinidade das lectinas por monossacarídeos é fraca. Entretanto, na natureza, essas interações são multivalentes. A multivalência está associada ao reconhecimento de múltiplas cópias de carboidrato apresentadas a múltiplas cópias de lectinas presentes na superfície celular.3 A multivalência aumenta a força e a seletividade das interações carboidrato-lectinas. 4 Substâncias multivalentes sintéticas derivadas de carboidratos, denominadas genericamente glicodendrímeros, 4 mimetizam a interação dos glicoconjugados com lectinas no sistema biológico. Há, portanto, grande interesse na obtenção dessas substâncias, para avaliação de suas interações com lectinas.1,4-8 Tem-se demonstrado que a eficiência das interações entre glicodendrímeros e lectinas depende de vários fatores, como o número de unidades de carboidratos presentes na molécula (multivalência), a distância entre essas unidades e a flexibilidade do dendrímero. [9][10][11] De modo geral, aumentando-se a multivalência aumenta-se a eficiência da interação carboidratolectina.12-14 Entretanto, isso nem sempre é o caso. Há diversos relatos em que dímeros, trímeros e tetrâmeros apresentam afinidade superior, quando comparados a análogos de maior valência. [15][16][17][18] Esse fato, aliado à simplicidade estrutural e baixo peso molecular dos dímeros, tem despertado o interesse em sua síntese. [18][19][20][21][22] .Recentemente relatamos a síntese de uma série de ligantes monoméricos derivados de D-galactose e lactose (4-O-(b-Dgalactopiranosil)-D-glicose) e a interação desses com a lectina de Erythrina cristagalli (LEC). 23 No presente trabalho é descrita a síntese de dois dímeros derivados de D-galactose, com o mesmo objetivo. Esses dímeros (estruturas 6 e 7, Figura ...

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Isolation, purification, and physicochemical characterization of a d-galactose-binding lectin from seeds of Erythrina speciosa

Cited by 62 publications

References 20 publications

Preliminary characterization of a Rhodnius prolixus hemolymph trypanolytic protein, this being a determinant of Trypanosoma rangeli KP1(+) and KP1(-) subpopulations' vectorial ability

Preliminary characterization of a Rhodnius prolixus hemolymph trypanolytic protein, this being a determinant of Trypanosoma rangeli KP1(+) and KP1(-) subpopulations' vectorial ability

Isolation and partial characterization of a D-galactose-binding lectin from the latex of Synadenium carinatum

Síntese de β-D-galactopiranosídeos de arila diméricos para avaliação de sua interação com a lectina de Erythrina cristagalli

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