Isolation, Production, and Characterization of Thermotolerant Xylanase from Solvent Tolerant<i>Bacillus vallismortis</i>RSPP-15

Gaur, Rajeeva; Tiwari, Soni; Rai, Priyanka; Srivastava, V. Tanmya

doi:10.1155/2015/986324

Cited by 20 publications

(9 citation statements)

References 30 publications

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“…Literature reports from previous studies reveal that the incubation time for optimal cellulase production by other Streptomyces species were in the range of 72-196 h [28,47,56,57]. Nevertheless, the findings of this study agree with that of other authors who reported 48 h as optimum for xylanase production [58][59][60]. However, most other studies recorded longer optimum incubation time for both cellulase and xylanase production using different substrates [27,36,45,61].…”

Section: Time Course and Kinetics For Production Of Cellulase And Xylsupporting

confidence: 81%

Classical Optimization of Cellulase and Xylanase Production by a Marine Streptomyces Species

2016

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Cellulase and xylanase are in high demand for application in several industrial processes, consequently necessitating the bioprospecting and manipulation of microbes for novel and greater enzyme productivity. This study reports on the optimal conditions for cellulase and xylanase production by a marine bacterial isolate from Nahoon beach sediment, via the classical process of one variable per time. Furthermore, the inducing effect of mono-and polysaccharides on enzyme production was investigated. The 16S rDNA gene sequence analysis clearly assigned the isolate to the genus Streptomyces, and was deposited at the GenBank under the accession number KU171373. Cellulase and xylanase production was optimal at the following conditions: pH 6 and 8, incubation temperature of 40 and 35 • C, and agitation speed of 100 and 150 rpm, respectively. Under optimum conditions, 0.26 U/mL and 18.54 U/mL activities were attained at 60 and 48 h with specific productivity of 205 and 7417 U/g for cellulase and xylanase, respectively. Xylanase production was induced by the entire mono-and polysaccharides tested, while cellulase production was induced by some. The results from this study signify the resourcefulness of the Streptomyces strain for production of cellulase and xylanase of industrial importance.

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Section: Time Course and Kinetics For Production Of Cellulase And Xylsupporting

confidence: 81%

Classical Optimization of Cellulase and Xylanase Production by a Marine Streptomyces Species

2016

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“…Similar findings with this study for the optimal initial pH 7 for xylanase production have been reported in previous studies on Bacillus species, including B. pumilus ASH, Bacillus altitudinis DHN8, B. amyloliquefaciens XR44A and B. vallismortis RSPP-15 (Sanghi et al, 2008;Adhyaru et al, 2014;Amore et al, 2013;Gaur et al, 2015). Contrary to this result however, optimal initial pH varied for xylanase production by B. megaterium (pH 7.5), B. subtilis (pH 8) and B. pumilus VLK-1 (pH 6), (Tandon and Sharma, 2014;.…”

Section: Effect Of Initial Ph On Holocellulase Productionsupporting

confidence: 91%

Optimization of Process Conditions for the Production of Holocellulase by a <i>Bacillus</i> Species Isolated from Nahoon Beach Sediments

Fatokun¹,

Nwodo²,

Olaniran³

et al. 2017

American Journal of Biochemistry and Biotechnology

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Production of holocellulase by a newly isolated marine Bacillus species via submerged fermentation technique at varying cultivation conditions was investigated. Enzyme production was optimized by altering one variable per time. Moreover effect of lignocellulosic saccharides in inducing cellulase and xylanase production was also investigated. The 16S ribosomal Deoxyribonucleic Acid (16S rDNA) gene sequence analysis exhibited 98% sequence similarity of the isolate with other Bacillus species in the gene bank and was deposited with the accession number KX524510. Optimal xylanase and cellulase production was attained at pH 7, temperature 30°C and agitation speed of 50 and 150 rpm. Furthermore, maximum xylanase and cellulase production were both achieved at 60 h corresponding to the late stationary growth stage, with activity of 16.6 and 0.061 U mL −1 respectively. Xylanase production was maximally induced by beechwood xylan, xylose and arabinose with activities of 13.59, 8.78 and 1.90 (U mL −1 ) respectively; while cellulase production was induced by carboxymethyl cellulose only and no cellulase activity was detected in the culture supernatant of the other carbon sources tested. Optimization increased cellulase and xylanase yields being 0.006-0.061 and 0.23-16.6 (U mL −1 ) from unoptimized to optimized respectively. The results of the study suggest the bacterial strain to be a proficient producer of cellulase and xylanase with potentials in biotechnological application.

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“…The biggest challenge in conversion of biomass with hyperaccumulated contaminants into fermentable sugars is the inhibition of hydrolytic enzymes by absorbed metal contaminants [ 7 ]. Because of the complexity of dry biomass and presence of metal contaminants, it is difficult to hydrolyze a hyperaccumulator efficiently with a single enzyme system [ 8 , 9 ]. Therefore application of a crude enzyme cocktail may be an efficient strategy to hydrolyze metal-contaminated biomass [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

Metal accumulation by sunflower (Helianthus annuus L.) and the efficacy of its biomass in enzymatic saccharification

Dhiman

Zhao

et al. 2017

PLoS ONE

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Accumulation of metal contaminants in soil as a result of various industrial and anthropogenic activities has reduced soil fertility significantly. Phytoextraction of metal contaminants can improve soil fertility and provide inexpensive feedstock for biorefineries. We investigated the hyperaccumulation capacity of sunflower (Helianthus annuus) biomass by cultivating these plants in various concentrations of metal contaminants. Sunflowers were grown in soils contaminated with various levels of heavy metals (10–2,000 mg/kg dry soil). The degree of metal uptake by different parts of the biomass and the residual concentration in the soil were estimated through inductively coupled plasma mass spectrometry. An almost 2.5-fold hyperaccumulation of Zn2+ was observed in the leaf and flower biomass compared with the concentration in the soil. For the subsequent saccharification of biomass with hyperaccumulated contaminants, a fungal lignocellulosic consortium was used. The fungal consortium cocktail retained more than 95% filter paper activity with 100 mM Ni2+ ions even after 36 h. The highest saccharification yield (SY, 87.4%) was observed with Ni2+ as the contaminant (10 mg/kg dry wt), whereas Pb2+ (251.9 mg/kg dry wt) was the strongest inhibitor of biomass hydrolysis, resulting in only a 30% SY. Importantly, the enzyme cocktail produced by the fungal consortium resulted in almost the same SY (%) as that obtained from a combination of commercial cellulase and β-glucosidase. Significant sugar conversion (61.7%) from H. annuus biomass hydrolysate occurred, resulting in the production of 11.4 g/L of bioethanol. This is the first study to assess the suitability of phytoremediated sunflower biomass for bioethanol production.

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Isolation, Production, and Characterization of Thermotolerant Xylanase from Solvent TolerantBacillus vallismortisRSPP-15

Cited by 20 publications

References 30 publications

Classical Optimization of Cellulase and Xylanase Production by a Marine Streptomyces Species

Classical Optimization of Cellulase and Xylanase Production by a Marine Streptomyces Species

Optimization of Process Conditions for the Production of Holocellulase by a <i>Bacillus</i> Species Isolated from Nahoon Beach Sediments

Metal accumulation by sunflower (Helianthus annuus L.) and the efficacy of its biomass in enzymatic saccharification

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