Isolation of yeast from soil and different food samples and its characterization based on fermentation

Thapa, Sandeep; Shrestha, Rajani; Tirewal, Anjali; Sharma, Arjun D.; Yuvraj, K C

doi:10.3126/njb.v3i1.14226

Cited by 6 publications

(3 citation statements)

References 12 publications

(8 reference statements)

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“…On EMB agar E.coli showed dark greenish metallic sheen colonies, on MSA Staphylococcus aureus showed golden yellow colonies and Staphylococcus epidermidis showed pink colonies, on MacConkey agar Klebsiella produced pink lactose fermented colonies, Salmonella sp produced black colonies on salmonellashigella gar, on Bile Aesculin agar Listeria sp produced black colonies, on Nutrient agar Bacillus showed white colonies, Candida sp showed white colonies on yeast extract peptone dextrose agar, Trichoderma sp produced light green conidia cover the entire media and the initial growth of Aspergillus sp shown as white in color. It was also observed by (Faryal et al, 2006;Ohgiwari et al, 1992;Prakasam et al, 2017;Thapa et al, 2015).…”

Section: Isolation Of Microbes From Municipal Solid Wastesupporting

confidence: 57%

Molecular and biochemical characterization of solid waste biodegrading microbes isolated from municipal waste dump site

Afshan,

Ghouri,

Hayat

et al. 2023

Acta Bras.

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Solid waste dump site-based pollution is one of the colossal health concerns globally due to presence of cellulose, starch, protein, and lipids, which is a perfect nutrient waste for the development of biodegrading potential microbes. The current study aimed to determine the productive microorganisms for waste biodegradation. Ten samples were collected from different areas of Karachi waste dump sites. The identification and characterization of isolates were done by morphological and biochemical tests. The isolated microbes were qualitatively screened for the action of making industrially key bio-enzymes. The potential enzymes producing microbes were subjected to waste degradation test for 90 days. Changes of odor, color and weight loss of decomposing garbage were noted. The highest degradation ability in term of weight loss was shown by Aspergillus (SSI14) and Bacillus (SSI6). Four metals (zinc sulphate, cadmium chloride, copper sulphate and iron sulphate) were chosen for the purpose of metals tolerance test and minimum inhibitory concentration (MIC) of the isolated microbial strains. Current study reported that Zn was found to be more lethal in in comparison to Cu and Fe. Molecular identification of the isolated microbes was done by polymerase chain reaction. The microbial strains were characterized by the polymerase chain reaction (PCR) followed by confirmation of product by agarose gel electrophoresis. The findings can be served as baseline data to develop microbial apparatus for biodegradation and management of solid waste.

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Section: Isolation Of Microbes From Municipal Solid Wastesupporting

confidence: 57%

Molecular and biochemical characterization of solid waste biodegrading microbes isolated from municipal waste dump site

Afshan,

Ghouri,

Hayat

et al. 2023

Acta Bras.

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“…Yeasts are found to colonize in diverse habitats, some are beneficial and some are infectious. Some of the yeasts are widely exploited for commercial purposes as they can be easily manipulated genetically [2,3]. Yeasts have wider applications in fields such as food, medicine and biotechnology research but their potential has been exploited lesser in crop improvement practices [4].…”

Section: Introductionmentioning

confidence: 99%

Estimation of Indole Acetic Acid and Phosphate Solubilisation by the Yeasts Isolated from the Leguminous Crops

Archana

Mallesha

2021

CJAST

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Aim: The present study was aimed to estimate the quantity of IAA production and Phosphate solubilisation by the isolated yeasts from the leguminous crops. Place and Duration of Work: The experiments were carried out in the Department of Agricultural Microbiology, University of Agricultural Sciences, GKVK, Bengaluru during 2019-2020. Methodology: Yeasts were isolated from the leguminous crops such as Red gram, Cowpea, Green gram, Black gram and Bengal gram. Thirty-five yeast isolates were obtained using four media and were subjected to IAA production test and Phosphorus solubilisation by Spectrophotometry method. All the yeast isolates were subjected to the temperature tolerance test at 25, 30, 35 and 40°C. Results: Yeast isolate, CP15SI21 has found to produce the highest IAA under in vitro conditions 30.50 μg/ml and the lowest was found to be 17.16μg/ml by yeast isolate BG20SI29. 24 yeast isolates were found to produce above 20 μg/ml. In the case of Phosphate solubilisation highest was observed in GG7SI9 (25.70 mg/l) and lowest in BG6SI8 (1.20 mg/l). Seven isolates showed Phosphate solubilisation above 10 mg/l. At 35°C all the yeast isolates have shown very good growth compared to other lower temperatures. All the yeast isolates were found to grow in the media supplemented with micronutrients such as zinc and potassium. Conclusion: Our study highlights the potential of yeasts isolated from the leguminous crops that can help in plant growth promotion as the yeast isolates are capable of producing higher amounts of IAA. Some of the yeast isolates can solubilise phosphate under in vitro conditions which in turn helps in the utilization of unavailable P from soils thereby improves plant growth and tolerance to a higher temperature can alleviate abiotic stress.

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“…Yeast is widely distributed in nature with a variety of habitats and is commonly found onplant leaves, flowers and fruits, as well as in soil 19 . However, there is few strains which have better fermentative characteristic, therefore there is always a need with better features in bioethanol fermentation especially high ethanol tolerance and production on commercial scale.…”

Section: Introductionmentioning

confidence: 99%

Isolation and Evaluation of Comparative Ethanol Production Capacity of Yeasts in Presence of Different Carbohydrate Sources from Soil

K Dash,

R Swain,

L Sahoo

et al. 2017

J. Adv. Mircobiol.

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Soil, is an enriched source of natural fermenting microorganisms including yeasts, which are capable of producing bioethanol. The present study was carried out to isolate and identify yeast strains from soil and screentheir ethanol tolerance capacity with a viewto select efficient yeast strains for industrial bioethanol production. Twelve yeast strains (YS1-YS12) were isolated from soil. On the basis of morpho-physiological characteristics and biochemical characterization, these yeast strains were identified as six strains of S.cerevisiae (YS1, YS2, YS4, YS5, YS7 and Y10), three strains of Candidaalbicans (YS3, YS8 and YS9) and three strains each of Trycosporoncapitatum (YS6, YS11 and YS12). Screening of ethanol tolerance capacity of these strains revealed that the two strains YS2 andYS3 tolerated ethanol concentration up to 12 % (v/v), while other two isolated strains YS1 and YS5 showed tolerance up to 10 % of ethanol concentration. The remaining eight strains showed tolerance of 9 % (v/v). Further these identified strains were evaluated for their comparative bioethanol production capacity in different carbohydrate substrates (grape juice, mahua flower extract, molasses, sugarcane juice and saccharified sweet potato root flour broth). Taking different strains and substrate conditions into account, strain YS2 showed in overall the highest ethanol production and ethanol tolerance capacity in comparison to the other strains indicating as an efficient strainfor its use in ethanol production purpose.

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Isolation of yeast from soil and different food samples and its characterization based on fermentation

Cited by 6 publications

References 12 publications

Molecular and biochemical characterization of solid waste biodegrading microbes isolated from municipal waste dump site

Molecular and biochemical characterization of solid waste biodegrading microbes isolated from municipal waste dump site

Estimation of Indole Acetic Acid and Phosphate Solubilisation by the Yeasts Isolated from the Leguminous Crops

Isolation and Evaluation of Comparative Ethanol Production Capacity of Yeasts in Presence of Different Carbohydrate Sources from Soil

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