Isolation of verotoxigenicEscherichia coli O157 from poultry

Abstract

Results obtained by examination of cloacal swabs from poultry for the presence of verotoxigenic strains of E. coli O157:H7 are presented. Twenty samples (9.2%) of 216 samples examined were positive for E. coli O157. Out of 20 E. coli O157, 19 strains were positive for the production of both verotoxins (VT1 and VT2). However, none of them was positive for the presence of H7 antigen.

Investigation of shiga toxin-producing Escherichia coli in avian species in India

“…In the present investigation, although three (0AE7%) of 426 strains belonged to O157 serogroup, none of these was positive for stx or any other genes studied. This corroborates with the findings of Doyle and Schoeni (1987) Pilipcinec et al (1999) reported that 20 of 216 cloacal swabs taken from poultry in Slovakia, were culture positive for STEC O157 and Wallace et al (1997) isolated STEC O157 from faecal samples of wild birds (mainly gulls) in UK.…”

“…(1999) did not detect any E. coli O157 in 70 samples of fresh droppings of domestic pigeons and sparrows in Czech Republic. However, Pilipcinec et al. (1999) reported that 20 of 216 cloacal swabs taken from poultry in Slovakia, were culture positive for STEC O157 and Wallace et al.…”

“…There are many reports of isolation and characterization of STEC from cattle and sheep which are considered as the main reservoirs (Gioffre et al 2002;Sharma 2002;Moreira et al 2003;Wani et al 2003) but other animals like goats, horses, dogs, deer do also harbour these bacteria (Paton and Paton 1998a;Staats et al 2003). With regard to poultry, a limited number of surveys investigating the prevalence and characteristics of STEC in the live animal and on processed poultry meat have been published across the globe (Doyle and Schoeni 1987;Pilipcinec et al 1999;Morabito et al 2001). In India, there seems no report available on prevalence and molecular characteristics of STEC in avian species.…”

Antibacterial action of chitosan-arginine against Escherichia coli O157 in chicken juice

“…One potentially lethal human pathogen found in chicken is verocytotoxin-producing Escherichia coli (VTEC), which constitutes one of the most severe causes of food-borne infections on a global scale, particularly in Europe and the USA (Mead, 2004). E. coli O157 was found in 1.5% of poultry carcasses from Wisconsin, USA (Doyle & Schoeni, 1987), and in 9.3% of cloacal swabs taken from poultry at a slaughterhouse in Slovakia (Pilipcinec, Tkacikova, Naas, Cabadaj, & Mikula, 1999). In relation to the subsequent risk for human infection, E. coli O157:H7 cells are known to survive well in chicken breasts kept at À20 C for more than 18 months (Conner & Hall, 1994;Watabe et al, 2008).…”

Effects of storage temperatures and essential oils of oregano and nutmeg on the growth and survival of Escherichia coli O157:H7 in barbecued chicken used in Iran

“…Doyle and Schoeni (1987) demonstrated the presence of E. coli O157:H7 on 1.5% of poultry carcasses from Madison, WI, USA. Pilipcinec, Tkacikova, Naas, Cabadaj, and Mikula (1999) reported that 20 out of 216 cloacal swabs taken from poultry at a slaughterhouse in Slovakia were culture positive for Shiga-toxin positive E. coli O157. Beery, Doyle, and Schoeni (1985) suggested that chickens are a possible reservoir of E. coli O157:H7 as the organism can colonize in the ceca of chickens.…”