Isolation of Trypanosoma Cruzi from Blood of Acutely and Chronically Infected Mice in Tissue Culture

Martinez-Silva, R; Lopez, Vincent; Colón, Julio I.; Chiriboga, J.

doi:10.4269/ajtmh.1969.18.878

Cited by 7 publications

(3 citation statements)

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“…Its host range is very broad, with more than 200 vertebrate species serving as animal reservoirs (1), and several species of reduviid insects as vectors (1, 2). In vitro, this protozoan can infect many cell types from different origins (3,4).The parasite's cycle in the vertebrate host begins when metacyclic trypomastigotes are released with the feces of the insect, reach a skin wound or a mucosa, and enter cells. The parasites are initially found in phagocytic vacuoles, which they subsequently leave to multiply free in the cytosol.…”

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The exit of Trypanosoma cruzi from the phagosome is inhibited by raising the pH of acidic compartments.

Ley

Robbins

Nussenzweig

et al. 1990

The Journal of Experimental Medicine

163

109

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The protozoan parasite Trypanosoma cruzi can infect many distinct mammalian cell types. The parasites enter cells through the formation of phagocytic vacuoles, but later are found free in the cytosol, where they multiply as amastigotes. Using transmission electron microscopy we found that within 2 h after infection 70% of the parasites, including examples of both mammalian forms (trypomastigotes and amastigotes), were inside partially disrupted vacuoles or free in the cytosol. We demonstrated that the pH of vacuoles containing recently interiorized parasites is acidic, through immunocytochemical localization of the acidotropic compound DAMP (18) in their interior. Increasing the vacuolar pH with chloroquine, ammonium chloride, methylamine, or monensin significantly inhibited the escape of the parasites into the cytosol. These results are compatible with the hypothesis that an acid-active hemolysin of T. cruzi (15) might be involved in the escape mechanism.

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confidence: 99%

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confidence: 99%

The exit of Trypanosoma cruzi from the phagosome is inhibited by raising the pH of acidic compartments.

Ley

Robbins

Nussenzweig

et al. 1990

The Journal of Experimental Medicine

163

109

View full text Add to dashboard Cite

show abstract

“…Erste Versuche der Anreicherung von T. cruzi in Tumorzellinien waren erfolgreich (20). Das Verfahren ist für die Routinediagnostik jedoch nicht geeignet.…”

Section: Parasitologische Diagnostikunclassified