Isolation of transposon TnA from plasmid RP4 carrying two copies of this element

Dobritsa, Anatoly P.; Ksenzenko, V. N.; Fedoseeva, V. B.; Alexandrov, A. A.; Kamynina, T.P.; Khmelnitsky, Michael

doi:10.1016/0378-1119(80)90034-7

Cited by 6 publications

(3 citation statements)

References 22 publications

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“…The Ti plasmid from A. tumefaciens strain Ach5 used in this study differed slightly from the Ti plasmid harbored by strain B6-806 in that under our conditions the doublets SmaI 14ab and 16ab could be resolved in two separated bands. In addition, on the basis of deletion analysis, the map positions of SmaI fragments 12 and 14a were interchanged (29), correcting the published data for B6-806 (7).…”

Section: Resultsmentioning

confidence: 99%

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Characterization of Tn904 insertions in octopine Ti plasmid mutants of Agrobacterium tumefaciens

Ooms¹,

Klapwijk²,

Poulis³

et al. 1980

J Bacteriol

165

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Seven Tn904 insertion mutants of pTi Ach5 affecting Agrobacterium tumefaciens virulence were studied. The mutant character was shown to be plasmid borne. Four of these mutants were avirulent and carried an insertion in restriction endonuclease HpaI fragment 12, a 3.3-megadalton fragment, which therefore appears to be a Ti plasmid region essential for virulence. Two mutants were attenuated in virulence. The inserts mapped close to HpaI fragment 12. One mutant giving rise to small tumors with excessive adventitious root formation on Kalanchoe daigremontiana carried an insertion in the right side of the common sequence in the deoxyribonucleic acid of the Ti plasmid detected in crown gall tumors. The insertion behavior of Tn9O4 was studied by analyzing 11 independently isolated and randomly chosen mutants. The Tn9O4 inserts did not affect oncogenicity, tumor morphology, bacterial transfer functions, octopine catabolism functions, or vital parts of the Ti plasmid, such as the origin of replication. Most

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Section: Resultsmentioning

confidence: 99%

“…tumor formation. The weakly virulent mutant strain LBA4228(pAL245) had one of the Tn9O4 insertions in HpaI fragment 6, close to HpaI fragment 12 (Fig. 2).…”

Section: Fig 3 Tumors On K Daigremontiana Stemsmentioning

confidence: 99%

Characterization of Tn904 insertions in octopine Ti plasmid mutants of Agrobacterium tumefaciens

Ooms¹,

Klapwijk²,

Poulis³

et al. 1980

J Bacteriol

165

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“…To our knowledge this is the first reported isolation of such a plasmid from a natural isolate as distinct from a laboratory construct. The copies of T n l are present as inverted repeats, as are those in plasmids constructed in vitro (Robinson et al, 1978) and in vivo (Dobritsa et al, 1980;Robinson et al, 1978). Plasmid pUB2660 differs from the cryptic plasmid pUB2659 (CPT-A) only with respect to the two copies of T n l and therefore it is reasonable to conclude that pUB2660 is derived from a CPT-A-type plasmid by dual insertion of Tnl.…”

Section: Discussionmentioning

confidence: 99%

Evolution of an R Plasmid from a Cryptic Plasmid by Transposition of Two Copies of Tn1 in Providencia stuartii

Hawkey¹,

Bennett²,

Hawkey³

1985

Microbiology

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Examination of a series of isolates of Providencia stuartii collected over an 18 month period from a chronic-care patient at Bristol Royal Infirmary revealed the emergence of resistance to carbenicillin. Resistance was mediated by a 47 kb plasmid which transferred by conjugation to a plasmid-free strain of P. stuartii but not to Escherichia coli. Carbenicillin-sensitive isolates were either plasmid-free or contained a 36 kb cryptic plasmid. Restriction endonuclease mapping of this plasmid showed it to be closely related to 32 kb and 34 kb cryptic plasmids reported previously in P. stuartii from Bristol. Mapping of the R plasmid showed it to be derived from the 34 kb cryptic plasmid by transposition of two copies of Tn1.

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IS8- and Tn2353-mediated cointegration of the plasmids R15 and RP4:: Tn1

Dobritsa¹

1984

Molec. Gen. Genet.

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The plasmids R15 and RP4::Tn1 form fused structures (85 Md and 92 Md cointegrates). The cointegrates do not resolve practically in recA- Escherichia coli cells and have a mean life-time of more than 50 generations in a recA+ background. The 85 Md cointegrates were generated at a frequency of 4 x 10(-4) per R15 transconjugant during a mating between E. coli [R15; RP4::Tn1] and E. coli [F' ColVBtrp :: Tn1755 ]. These plasmids carry two directly repeated copies of the mobile element IS8 at the junctions between R15 and RP4::Tn1. The transposition of IS8 from RP4::Tn1 to the R15 plasmid and the formation of hybrid molecules promoted by this process appear to be induced by the IS8 element of the Tn1755 structure during or after conjugal transfer of F' ColVBtrp :: Tn1755 into E. coli [R15; RP4::Tn1] cells. The formation of the 92 Md cointegrates occurs at a frequency of 2 x 10(-5). The fused molecules of R15 and RP4::Tn1 carry two direct copies of an 8.65 Md R15 fragment at the junctions between these replicons. The fragment has specific features of a new transposon. This element designated Tn2353 determines resistance to Hg, Sm and Su and contains two sites for each BamHI, Bg/II and Sa/I and three sites for both EcoRI and PstI. The physical map and some other characteristics of Tn2353 are presented.

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Isolation of transposon TnA from plasmid RP4 carrying two copies of this element

Cited by 6 publications

References 22 publications

Characterization of Tn904 insertions in octopine Ti plasmid mutants of Agrobacterium tumefaciens

Characterization of Tn904 insertions in octopine Ti plasmid mutants of Agrobacterium tumefaciens

Evolution of an R Plasmid from a Cryptic Plasmid by Transposition of Two Copies of Tn1 in Providencia stuartii

IS8- and Tn2353-mediated cointegration of the plasmids R15 and RP4:: Tn1

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