Isolation of three antibacterial naphthoquinones from <i>Plumbago indica</i> roots and development of a validated quantitative HPLC analytical method

Kaewbumrung, Sermwut; Panichayupakaranant, Pharkphoom

doi:10.1080/14786419.2011.628670

Cited by 21 publications

(10 citation statements)

References 9 publications

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“…Ethanol was the most suitable solvent for P. indica root extraction than ethyl acetate, isopropanol, dichloromethane and diethyl ether. Ethanol has given high yield of the crude extract (11.5% w/w) when compare with other solvents [40].…”

Section: Plumbaginmentioning

confidence: 97%

Propagation, Phytochemistry and Pharmacology of Plumbago indica - A Review

Priyanjani

Senarath

et al. 2021

JPRI

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Aims: Plumbago indica is a medicinal plant widely grown in the tropics and temperate region to use in traditional systems of medicine. Different parts of this plant are used to treat rheumatoid arthritis, dysmenorrhea, cancer, leprosy, syphilis, rheumatism, paralysis, headache, leukoderma, enlarged glands, scabies, ophthalmia, dyspepsia, haemorrhage, piles, flatulence, loss of appetite etc. Various kinds of researches were carried out in different countries on propagation and pharmacology of this medicinal plant. Scientifically proven data were concentrated on this paper to facilitate reliable convenience data source and encourage further studies. Methodology: Scientific data published on full papers, abstracts about morphology, growth, propagation, traditional medicinal uses and pharmacology of P. indica over 70 years (1949-2020) by researchers in different countries were collected and categorized under suitable topics. Results: According to literature P. indica leaves, stems, roots contain phytochemical compound that are responsible for its medicinal properties. Tissue culture of this plant can be uses as effective propagation method to fulfill the increasing demand of raw materials (dried plant parts) for medicinal preparations as well as preserve the plant in their natural habitat. Value: P. indica is used to treat vast range of diseases in traditional medicinal systems in different countries and currently pharmacological experiments are conducting to prove it scientifically. Plumbagin present in P. indica roots was already identified as a potential anti-cancer agent and subjected to more research interest. Therefore this review article helps new researchers to get wide knowledge about the plant and its pharmacology.

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Section: Plumbaginmentioning

confidence: 97%

Propagation, Phytochemistry and Pharmacology of Plumbago indica - A Review

Priyanjani

Senarath

et al. 2021

JPRI

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“…Plumbagin has gained international popularity mainly as a bioactive compound in marketed herbal medicines. It has been shown to have various pharmacological properties such as anticancer , antimicrobial , antiprotozoal , anthelmintic , and antifertility activities. As a consequence there has been an increasing demand for its availability, in particular for experimental purposes and by the pharmaceutical industry.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous heat shock and in situ adsorption enhance plumbagin production in Plumbago indica root cultures

Jaisi

Panichayupakaranant

2016

Engineering in Life Sciences

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Plumbago indica L. is an important source of plumbagin, a commercially valuable bioactive compound. However, the uses of plumbagin are limited due to its low supply as well as low yields and slow growth of the plant sources. This study evaluated the use of a simple, easy, and low-cost approach using heat shock (HS) and ultrasound (US), and an in situ adsorption using a nonpolar copolymer adsorbent styrene-divynilbenzene resin (Diaion R HP-20) to enhance plumbagin production in Plumbago indica root cultures. Treatment with HS (60°C) for 10 min significantly increased the production of plumbagin (5.51 mg/g DW) by up to five-fold, compared to the level in untreated root cultures (1.14 mg/g DW). In contrast, treatments with US alone or with HS treatment produced no satisfactory increase of plumbagin production. However, combined treatment of a 20-day-old root culture with HS (60°C, for 10 min) in the presence of Diaion R HP-20 (10 g/L) markedly increased the production up to 20.28 mg/g DW of plumbagin that was almost 14-fold higher, compared to the level in an untreated root culture. Such an increase would be sufficient for commercial applications of this method to produce plumbagin.

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“…The filtrates were evaporated to dryness under reduced pressure (40 C). The residues were reconstituted in methanol and the volume adjusted to 5 mL (Kaewbumrung & Panichayupakaranant, 2012). Samples were filtered through a 0.45 mm membrane filter and subjected to quantitative HPLC analysis for plumbagin content.…”

Section: Extraction Of Plumbagin From the Root Culturesmentioning

confidence: 99%

“…Quantitative HPLC analysis of plumbagin HPLC analysis was carried out using the method described by Kaewbumrung and Panichayupakaranant (2012). HPLC analysis was carried out using a binary HPLC pump …”

Section: Extraction Of Plumbagin From Liquid Mediamentioning

confidence: 99%

“…It is commercially important as a major source of plumbagin ( Figure 1). Plumbagin is a naphthoquinone compound that possesses a broad range of pharmacological activities such as anticancer (Wang et al, 2008), antimicrobial (Kaewbumrung & Panichayupakaranant, 2012;Park et al, 2006), anti-protozoal (Fournet et al, 1992), anthelmintic (Atjanasuppat et al, 2009) and antifertility (Kini et al, 1997) activities. Nowadays, P. indica roots are being used increasingly as ingredients in marketed phytomedicines.…”

Section: Introductionmentioning

confidence: 99%

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Increased production of plumbagin inPlumbago indicaroot cultures by gamma ray irradiation

Jaisi¹,

Sakunphueak

Panichayupakaranant

2013

Pharmaceutical Biology

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Context: Plumbagin is a major active constituent of Plumbago indica L. (Plumbaginaceae). It possesses various pharmacological activities that have been shown to assist in the treatment of various diseases. Objectives: This work is focused on increasing the production of plumbagin in P. indica root cultures using low doses of gamma ray irradiation as an elicitor. Materials and methods: The effect of low doses of gamma ray irradiation (0, 5, 10, 15, 20, 25 Gy) and ages of the root cultures (0, 5, 10, 15, 20 days) for elicitation of plumbagin production was determined. The stability of the elicited root cultures to produce plumbagin was also determined during three cycles of subculture. Results and discussion: Treatment of the root cultures with a low dose of gamma ray at 20 Gy gave the highest level of plumbagin production (1.04 mg/g DW) when compared to all other treated groups. The appropriate age of the root cultures for maximum production of plumbagin was found to be 10 days. However, treatment of 5-day-old root cultures resulted in a significant increase of dried root biomass that also had a high plumbagin production. Based on the total biomass per culture flask, the amounts of plumbagin produced by the 5-and 10-day-old treated roots were 0.59 and 0.37 mg/250 mL flask, respectively, which were 4.2-and 2.6-fold higher than the level in the control. Subculturing the root cultures until the third generation still showed an increase in plumbagin production without any effects on their growth.

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Isolation of three antibacterial naphthoquinones from Plumbago indica roots and development of a validated quantitative HPLC analytical method

Cited by 21 publications

References 9 publications

Propagation, Phytochemistry and Pharmacology of Plumbago indica - A Review

Propagation, Phytochemistry and Pharmacology of Plumbago indica - A Review

Simultaneous heat shock and in situ adsorption enhance plumbagin production in Plumbago indica root cultures

Increased production of plumbagin inPlumbago indicaroot cultures by gamma ray irradiation

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