Increased production of plumbagin inPlumbago indicaroot cultures by gamma ray irradiation

Abstract

Context: Plumbagin is a major active constituent of Plumbago indica L. (Plumbaginaceae). It possesses various pharmacological activities that have been shown to assist in the treatment of various diseases. Objectives: This work is focused on increasing the production of plumbagin in P. indica root cultures using low doses of gamma ray irradiation as an elicitor. Materials and methods: The effect of low doses of gamma ray irradiation (0, 5, 10, 15, 20, 25 Gy) and ages of the root cultures (0, 5, 10, 15, 20 days) for elicitation of plumbagin production was determined. The stability of the elicited root cultures to produce plumbagin was also determined during three cycles of subculture. Results and discussion: Treatment of the root cultures with a low dose of gamma ray at 20 Gy gave the highest level of plumbagin production (1.04 mg/g DW) when compared to all other treated groups. The appropriate age of the root cultures for maximum production of plumbagin was found to be 10 days. However, treatment of 5-day-old root cultures resulted in a significant increase of dried root biomass that also had a high plumbagin production. Based on the total biomass per culture flask, the amounts of plumbagin produced by the 5-and 10-day-old treated roots were 0.59 and 0.37 mg/250 mL flask, respectively, which were 4.2-and 2.6-fold higher than the level in the control. Subculturing the root cultures until the third generation still showed an increase in plumbagin production without any effects on their growth.

Plumbago indica L. Root Extract Induces Anti-proliferation, Anti-migration and Apoptosis on the Human Lung Cancer Cell Line

“…1 Furthermore, various pharmacological activities of plumbagin have been shown to assist in the treatment of cancer as well. 2 However, the data of PI mechanism on cancer treatment is still less information, therefore, the study of PI on cancer activities are urgently needed to explore.…”

Fungal endophytes of Plumbago zeylanica L. enhances plumbagin content

“…In past various people attempted to enhance the contents of the Plumbagin in Plumbago sp. using various methods e.g., generation of transgenic lines (Martin et al 2011), elicitation and in situ adsorption (Komaraiah et al 2002; Jaisi and Panichayupakaranant 2016), production of mutants using induced mutation (Jaisi et al 2013), developing root suspension culture (Roy and Bharadvaja 2019), triggering the pathways using bacterial treatment to the roots at in vivo level (Andhale and Ade 2013), and treatment of plant roots with fungal endophytes (Pandey et al 2016). Among all the methods, the technique which employed fungal endophytes, seems cost-effective, accepted and efficient method to enhance secondary metabolite contents in the plants.…”